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Description
Rat ICAM2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Intercellular Adhesion Molecule 2 (ICAM2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Intercellular Adhesion Molecule 2 (ICAM2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Intercellular Adhesion Molecule 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102), encodes a protein that is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins containing two to nine immunoglobulin-like C2-type domains that bind to the leukocyte adhesion protein LFA-1. ICAM-2 regulates sperm adhesion to Sertoli cells on the apical side of the blood-testis barrier (facing the lumen), thereby playing a vital role in spermatogenesis. This protein may also play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates antigen-specific immune responses, NK cell-mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune responses and surveillance. It has been shown to interact with EZR. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.3 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
2 Early Kubrick Films in 1 Thrilling Package
The most important thing for the buyer to know about The Killing Criterion Blu-Ray is that it collects 2 of Stanley Kubrick’s earlier films: The Killing (1956) and Killer’s Kiss (1955). Even though the Killer’s Kiss title is not on the box, it is present as an extra, along with its trailer.
Other than that, you can expect the usual from a Criterion Collection release. For those who don’t know, that means terrific quality and presentation, with loads of extra supplementary material.
As far as the movies themselves go — unlike Fear and Desire, I can actually recommend these two movies as movies, as opposed to morbid curiosities of Kubrick fanatics. They are both pretty intense crime thrillers and just a ton of fun. This is Kubrick before he makes big studio movies with big budgets, but after he’s gained more of the experience and sophistication he would later be known for. The product feels raw, gritty, sometimes intimate, and gives you a look at his NYC roots. Kubrick is still an underdog at this stage in his career. So, would definitely recommend for Kubrick fans, and just crime thriller fans in general
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Reviewed in the United States on December 11, 2022
★★★★★ 5
The Mastery of Stanley Kubrick
We're all children of Kubrick aren't we? Is there anything that you can do that he hasn't done? - Paul Thomas Anderson.
One of the most true statements about a great director from another great director. Stanley Kubrick's The Killing is a harsh piece of filmmaking that is the true definition of a film noir heist movie. The characters are not glamorous and they are not symphatetic. They are merely real and that is all you need in a movie. These characters move through the intricate plot with the masterful direction of Kubrick guiding them through it and speaking the crisp dialogue from pulp novelist Jim Thompson. With that combination The Killing is noir at its finest. A great early masterpiece from Kubrick. This Criterion Collection blu-ray also includes another early Kubrick work, Killer's Kiss, about a boxer who falls in love with a woman who lives next door to him and thus gets caught up in a dangerous game of cat and mouse with her shady boss. A great companion movie for The Killing. But there is one disappointment with this Criterion release. When it was first annouced that they were releasing this film, I had high hopes that they would release the rare Kubrick film Fear and Desire with this, but I was sadly disappointed. Maybe there is a seperate Criterion release for that film in the near future, we can only hope. But all in all this is a great blu-ray, highly recommended for fans of Kubrick or fans of the film noir genre.
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Reviewed in the United States on October 19, 2011
★★★★★ 5
Stanley Kubrick is a talented director.
If you are interested in the director Stanley Kubrick, you will enjoy his process of direction in this film from 1955.
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Reviewed in the United States on January 25, 2026
★★★★★ 5
Best glass skin mask that makes it look like you just had a facial!
Color: Lifting (Jericho Rose), Color: Lifting (Jericho Rose)
I bought this mask yesterday and used it last night for the first time. It instantly became part of my skincare regimen after just one use. I’ve tried a couple of different Korean skincare masks, but this one is already my top pick. First, it comes with the silicone applicator to help apply it evenly. It’s not sticky or tacky and it stays in place. The directions say wait 40 minutes and after 40 minutes it is dry and peels off like a normal face mask! It doesn’t pull at your skin when peeling it off, you don’t need to rinse it with water, and then the results are INSTANT. I was completely captivated in the shine it gave my skin. This will be a repeated purchase which sounds crazy after one use, but you can just tell it’s that good!! I included before and after (less than an hour time difference in these photos!)
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Reviewed in the United States on March 6, 2026
★★★★★ 5
After glow
Color: Lifting (Jericho Rose)
My skin looks glassy and feels very hydrated, best mask ever
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Reviewed in the United States on June 7, 2026
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