Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CCR4 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Chemokine CC-Motif Receptor 4 (CCR4) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Chemokine CC-Motif Receptor 4 (CCR4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Chemokine C-C-Motif Receptor 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | C-C chemokine receptor 4 (CCR4) is a protein encoded by the CCR4 gene. The protein encoded by this gene belongs to the G protein-coupled receptor family. Chemokines are a group of small, structurally related proteins that regulate cellular trafficking of various white blood cell types. Chemokines also play a fundamental role in the development, homeostasis, and function of the immune system. They influence cells of the central nervous system and endothelial cells involved in angiogenesis, or vascular dilation. CCR4 and CAF-1 form a delipidase complex that collaborates with the release factors eRF3 and PABPC1 to shorten the poly(A) tail of mRNA during translation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 16 reviews
Sort
Product Reviews
★★★★★ 3
Simple, Straightforward and Concise - Ideal for Beginners!
Format: Kindle
Written by Sophie Merle, The Book of Tapping: Emotional Acupressure with EFT is a short, concise and simple but straightforward guide to Tapping.
What I liked best about this book is that - it is very easy to understand, making it possible for anyone and everyone to explore EFT.
Since this is a very short book, I understand that not much is covered, just the basics predominantly, but if you are looking for an introduction to EFT Tapping and uncomplicated explanations and ideas, this is a book you can check out!
**I was offered an eARC by Healing Arts Press through Net Galley in exchange for an honest review.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2017
★★★★★ 5
An excellent basic text for anyone interested in learning the EFT system.
Format: Paperback
Over the years I have read a several books on the healing art of tapping and I must say this 106 page soft cover volume (The book of Tapping: Emotional acupressure with EFT by Sophie Merle) is one of the best I have read lately. The step-by-step lessons are easy to follow and everything is explained in a clear and concise way.
This book is organized into ten sections covering the following material: EFT: an energetic technique for health and material abundance, the EFT protocol, working with EFT, a collection of painful statements, the body’s meridians and their emotional function, the various ways EFT can be applied, EFT and the realization of goals, group sessions, two EFT variations and a conclusion.
If you are seeking a good basic introduction to the EFT system you may want to check out this excellent book.
Rating: 5 Stars. Joseph J. Truncale (Author: Chair/Seated Tai Chi, Qigong and Yoga stretching for seniors and the physically challenged).
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 2, 2019
★★★★★ 2
The Book of Tapping by Sophie Merle is a free ...
Format: Paperback
The Book of Tapping by Sophie Merle is a free NetGalley ebook that I read in early February.
The Book of Tapping discusses the use of EFT on the face, upper torso, and hands to loosen energy blockages along different meridians of the body and to relieve emotional stress in addition to physical ailments. There is mention of case studies, self-assessment techniques, a map of meridians, and glossary of EFT-related institutions and practitioners, but it's loosely held together by little else.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 9, 2017
★★★★★ 5
Delightful stories & antidotes!
Format: Paperback
Jackson Dunes has a delightful way of reminding us "big kids" what is truly important in life. I feel compelled to take time to smell the sea. Also perfect stories for children!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 5, 2015
★★★★★ 5
Fun Little Book
Format: Paperback
I love Pug! His simple observations of daily life lead to a powerful philosophy. Be yourself and take time to enjoy the simple pleasures of life are abundantly important to this simple puppy. I recommend this book for all who need a pick-me-up now and then - which is pretty much everybody!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2011
recommand products
WeatherTech 19-23 Dodge RAM 1500 CC (Incl. 21-23 TRX) Rear Side Window Deflectors - Dark Tint
44.99
Gram Lights 57CR Overseas Limited 18X9.5 +38 5x120 Admiral Blue
337.50
Gram Lights 57DR 18X9.5 +38 5x114.3 RBC Wheel (Special Order/Min Order Qty 20)
324.50
SPL Parts 03-08 Nissan 350Z Solid Differential Mount Bushings
161.99
WeatherTech 23-24 Honda HR-V Front FloorLiner HP - Black
148.99