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Description
Human CDHH ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a cadherin, heart (CDHH) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of cadherin, heart (CDHH) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cadherin, Heart ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cardiac cadherin, also known as T-cadherin, and cadherin 13 (CDH13) are unique members of the cadherin superfamily because they lack transmembrane and cytoplasmic domains and are anchored to the cell membrane via a GPI anchor. Classical cadherins are essential for cell-cell contacts, the dynamic regulation of morphogenetic processes in the embryo, and tissue integrity in the adult organism. Cadherins act as membrane receptors to mediate outward-directed signals, activating small GTPases and the β-catenin/Wnt pathway, leading to dynamic cytoskeletal reorganization and phenotypic changes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
I as well as my 6 year old use and like
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
This balm has been amazing for dry and irritated skin. I started using it on rough patches around my nose and cheeks, and it helped calm everything down quickly. The texture is thick but it absorbs better than I expected. A little goes a long way, so the tube lasts quite a while. I also like that it does not have a strong fragrance that irritates sensitive skin. It works well under makeup and overnight as a treatment. I will definitely keep repurchasing this because it has become a staple in my skincare routine.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
52, Post-Menopausal Dry Skin — This Is My Barrier Emergency Product
Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm, Size: 1.35 Fl Oz (Pack of 1), Style: Cicaplast Baume B5 Balm
I’m 52 with post-menopausal, mature dry skin, and this is not something I use casually or every day — it’s what I reach for when my skin barrier is compromised and needs real help. When my skin is irritated, over-exfoliated, reacting to actives, or feeling tight and inflamed, I don’t want glow or anti-aging claims. I want calm, protection, and repair. This balm delivers exactly that.
The texture is rich and occlusive, but not greasy or suffocating. It melts into the skin and immediately takes the edge off irritation — that tight, uncomfortable feeling just stops. Within minutes, redness looks calmer, dryness is softened, and my skin feels protected rather than coated. It creates a breathable barrier that actually stays put overnight, which is especially important on mature skin that loses moisture quickly.
What I appreciate most is that this works with my existing routine instead of competing with it. I use growth factors, peptides, and treatment serums regularly, and when my skin needs a reset, this seals everything in without stinging or interfering. I’ve used it after retinoids, during periods of sensitivity, and when my skin simply feels fragile — and it consistently helps restore balance.
That said, if you have very oily skin or are looking for a lightweight daytime moisturizer or a dewy “glass skin” finish, this probably isn’t the product for you. This is a true treatment balm, designed for barrier repair rather than cosmetic glow.
This is not a glow cream, a makeup primer, or a lightweight moisturizer, and that’s exactly why it’s so effective. It’s a true barrier balm. On my dry, post-menopausal skin, it reduces flaking, minimizes irritation, and helps my skin recover faster so I can get back to my normal routine without setbacks.
With so many skincare products making big promises, this one quietly does its job — and does it extremely well. If you have mature, dry, or reactive skin and need something reliable for barrier repair, this earns its place. It’s not exciting, it’s not trendy — it’s just very, very effective.
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Reviewed in the United States on February 6, 2026
★★★★★ 5
Umbrella
Color: Tan, Size: 10 Foot, Color: Tan, Size: 10 Foot
10ft umbrella was perfect very good quality
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Great umbrella for price
Color: Navy, Size: 10 Foot
Great price, seems durable, fast shipped
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Color was great
Color: Tan, Size: 9 Foot
Perfect patio umbrella, price was fantastic.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 18, 2026
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