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Description
Human NOS1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Nitric Oxide Synthase 1, Neuronal (NOS1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Nitric Oxide Synthase 1, Neuronal (NOS1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Nitric Oxide Synthase 1, Neuronal ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Nitric oxide synthase 1 (neuronal), also known as NOS1, is encoded by the NOS1 gene. Nitric oxide synthases (NOSs) are a family of synthases that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide is a chemical messenger with diverse functions throughout the body depending on its enzymatic source and tissue localization. NOS1 is primarily found in the brain and peripheral nervous system. NO exhibits many properties of a neurotransmitter and may participate in long-term potentiation. It has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neuromodulation of smooth muscle (including peristalsis and sphincter relaxation), and penile erection. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 4
no dog would resit these...
Flavor Name: Braided Stick (Chicken)
My dogs loved them but they were a lot smaller than I imagined.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 26, 2026
★★★★★ 5
Turkey tendon, vitamin E, low in fat, and high in protein. A great little midday snack 😊
Flavor Name: Braided Stick (Chicken)
My dog really liked these iPaw Turkey Tendon Braided Sticks. Every time I gave her one she went nuts and destroyed it in less than 5 mins. She's 80 pounds and for most medium-large dogs it will be the same, they won't last long at all. But I imagine for much smaller dogs they'd probably be a good 10-15 min chew. They have a very mild and almost non-existent smell, and they texture is quite hard and firm, so I would be hesitant to let a dog with missing teeth have one. They may be a bit difficult to chew. My dog hasn't had any upset stomach or diarrhea issues after eating these, which is always something I can keep an eye out for when feeding her something new. So far so good! They've got turkey tendon and vitamin E which are both super nutritional for dogs, and they're low in fat and high in protein which is another huge plus. Especially if your dog has weight issues. They're pretty small and they don't last long, but that's ok, as long as my girl loves them and enjoys them that's all that matters to me. They make for a great little midday snack, and for only $14 I would absolutely purchase them again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 22, 2026
★★★★★ 4
Chihuahua and Border Collie Approved
Flavor Name: Braided Stick (Chicken)
We got the iPaw Turkey Tendon Braided Sticks for our two senior pooches, and it’s safe to say they are an absolute hit in our household.
Both of our dogs seem to genuinely love the flavor and the "chew" of these. They stayed engaged with the treat until the very last bit was gone.
While they are hard, they aren't rock-hard. I was easily able to cut them up with scissors for our Chihuahua, who is missing several teeth. He was able to gum them down without any issues, which is a win for a pup with dental challenges.
The only reason I’m giving these 4 stars instead of 5 is the price. They are definitely on the expensive side for the amount you get. Since my dogs go through them so quickly, it’s hard to justify keeping these as a "constant" staple in the pantry, but they make for an excellent occasional high-value treat.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 10, 2026
★★★★★ 5
Dogs loved them, but gone very quickly
Flavor Name: Braided Stick (Chicken)
I picked up these turkey tendon braided sticks as a chew treat for my dogs, and they had zero hesitation. Both dogs were immediately interested and happily worked through them without any convincing. They seem very tasty and easy for dogs to chew, which makes them a nice occasional reward. They seem to be of healthy, quality ingredients, which is always a plus.
Similar to some other natural chews I’ve tried, they just don’t last very long with bigger or enthusiastic chewers. What looks like it might be a longer distraction turns into a fairly quick snack in our house. Still, the dogs enjoyed them and tolerated them well, so they work fine as a treat, just not a long lasting chew for large dogs.
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Reviewed in the United States on February 27, 2026
★★★★★ 3
Expensive for What You Get
Flavor Name: Braided Stick (Chicken), Flavor Name: Braided Stick (Chicken)
My dogs love chews and I like to try different kinds. I thought they would be longer lasting since they are braided and turkey tendon.
These come in a small bag with just a few chews. The chews aren't very big either. They are chewy but not so much that they take a while to eat. I gave one to my 36 lb labradoodle and he finished it in a couple minutes - so it isn't a long-lasting treat at all. At this price point, I would expect more treats or something that lasts a while. My dog loved these but I expect more bang for my buck.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 31, 2025
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