SKU: 42507682884

Human FGF Acidic ELISA Kit

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Description

Human FGF Acidic ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human FGF monoclonal antibody is coated on the microplate. The FGF in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human FGF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human FGF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.19 pg/ml

Detection Range: 125 - 8000 pg/ml

Recovery Range: 96-113%

Storage Method: 2-8 ℃

Standard Curve

Background:

Fibroblast growth factor acidic (FGF acidic), also known as FGF-1, endothelial cell growth factor (ECGF) and heparin binding growth factor-1 (HBGF-1) are non glycosylated 17-18 kDa polypeptides secreted by a variety of cell types. This molecule is synthesized as an amino acid without obvious signal peptide sequence(AA) protein. Therefore, this ruled out the possibility that it was secreted through the canonical er/ Golgi pathway. However, it seems to have a unique secretion pathway including heat shock protein, phosphatidylserine, synaptotagmin-1. The result of acidic secretion of FGF is that extracellular disulfide bonds connect homodimers, and its covalent bonds may be destroyed by reducing agents to release bioactive FGF acidity. Unlike FGF-2, acidic FGFs are known to have a 5'alternate start site. The acidic FGF of a 60 AA splice variant has been reported to be a full-length molecule consisting of the first 57 amino acids plus three additional C-terminal amino acids. In experiments, it can bind FGF receptors, but will not activate them. Acidic FGF has a nuclear localization sequence consisting of aa residues 21-27. Acidic FGF exhibits 95% AA properties for mice and rats and 92% AA properties for cattle. Cells known to express acidic FGFs include mammary epithelial cells and neurons (motor and sensory), skeletal muscle and smooth muscle cells, renal proximal tubular cells, endothelial cells, macrophages, keratinocytes, fibroblasts. The FGF family has five receptors (FGF R1-R5). The first four receptors are transmembrane tyrosine kinase receptors, and FGF R5 is a member of IgSF without tyrosine kinase domain. Notably, acidic FGFs have been reported to bind receptors 1 to 4, but not FGF R5, with FGFs. Acidic FGFs can activate tyrosine kinase binding or use the signal pathway of ligand receptor internalization to reach organelles and nuclei in cells. Receptor activation is associated with concurrent FGF heparin interactions. Heparin can bind with acidic FGF to inhibit or activate the receptor. When activated, heparin appears to be a central component connecting the two fgf/fgf r complexes. This is an approximation of two FGF r molecules to initiate receptor dimerization and signal transduction. Acidic FGFs also bind heparin on the cell surface, with internalization but no cell activation. Acidic FGF is famous for its mitotic activity on endothelial cells. Activation and internalization of membrane receptors are necessary for complete mitosis. Other cells that proliferate in response to acidic FGF include fibrocytes, muscle cells, hepatocytes, mammary epithelium and fibroblasts.

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SKU: 42507682884

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Joe Goob
West Palm Beach, US
★★★★★ 5
Very nice
Good price
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Reviewed in the United States on January 26, 2026
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Coffee Customer
Belleville, US
★★★★★ 5
Amazing lotion for the low price.
Size: 20.3 Fl Oz (Pack of 1)
Great everyday lotion and an excellent value for the price. The texture is light and creamy, not greasy, and it absorbs quickly. I like the scent, though scent preference is personal. Shipping was fast. The only downside is the pump—it was difficult to open and required turning it counterclockwise, then clockwise, which was more effort than it should have been. Once it popped up, it works perfectly.
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Reviewed in the United States on January 25, 2026
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mia lia
Draper, US
★★★★★ 5
Best non greasy moisturizing lotion ever!!
Size: 20.3 Fl Oz (Pack of 1), Size: 20.3 Fl Oz (Pack of 1)
This lotion is honestly probably one of the best lotions I’ve ever used. I used it for the first time today immediately after my shower and it’s the perfect consistency not too thick or thin.. not full of water either which is good because my skin absorbs instantly! Most lotions sit on my skin and make me greasy and sweaty afterwards. It also smells like HEAVEN. Chocolaty and a hint of vanilla! I won’t be buying any other lotion this now!
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Reviewed in the United States on April 14, 2026
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Karly P.
Cuba, US
★★★★★ 4
Great moisturizer
Size: 20.3 Fl Oz (Pack of 1), Size: 20.3 Fl Oz (Pack of 1)
This cream is a great alternative to the more expensive name brand creams. I have ordered this a few times and I feel like it does a wonderful job keeping my skin feeling soft, nourished and moisturized until the next time I shower. I do not feel as though my skin is greasy or oily after using this product. The cream does not feel thick or heavy on my skin. I am able to put a pair of pants on comfortably after using cream without feeling sticky. The cocoa butter scent is light and not over powering. There are parts of my body that I have to avoid with the cream because I do have eczema, but this is something I have grown up with and I am used to. I have purchased the cream twice, and unfortunately, the second time I bought it the pump top came a little bit destroyed (shown in photo). The product is still fully functional and will not determine from buying this again in the future!
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Reviewed in the United States on May 14, 2026
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Chillbilly
West Palm Beach, US
★★★★★ 5
Satisfied
Size: 20.3 Fl Oz (Pack of 1)
Great value. Lotion is non greasy - fast absorbing and will last thru handwashing, leaves skin revitalized and soft. The smell is not as pleasant as I would like and the bottle's size makes pumping difficult. But all in all it was a good buy and worth the cons.
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Reviewed in the United States on April 11, 2026

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