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Description
Mouse TNFSF14 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a capture antibody against Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Tumor Necrosis Factor Ligand Superfamily, Member 14 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tumor necrosis factor superfamily member 14 (TNFSF14), also known as LIGHT, is a secreted protein of the TNF superfamily. It is recognized by the herpesvirus entry mediator (HVEM) and decoy receptor 3. LIGHT stands for "homologous to lymphotoxin, exhibits inducible expression, and competes with HSV glycoprotein D for binding to the HVEM receptor expressed on T lymphocytes." The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.6-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 4
Great case, bad magnet.
Size: Iphone 17 Pro Max, Color: Dark Blue
The case itself is very good. It fits well on the phone. It’s not bulky at all and it seems pretty sturdy. The only problem is the magnet in the back is very weak. Besides that, it’s a great buy.
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Reviewed in the United States on May 31, 2026
★★★★★ 5
Sleek & Protective iPhone 17 Pro Case – Perfect Everyday Fit
Size: Iphone 17 Pro, Color: Orange, Size: Iphone 17 Pro, Color: Orange
This iPhone 17 Pro case fits perfectly and feels sturdy while still being lightweight. It gives good protection without making the phone bulky, and the design looks clean and premium. The buttons are easy to press and it feels comfortable to hold for everyday use.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 20, 2026
★★★★★ 5
Practical Protection meets everyday elegance
Size: Iphone 15, Color: Black
When it comes to phone cases, functionality often battles design, leaving users to compromise on one or the other. However, the Miracase Magnetic Series Designed for iPhone 15 strikes an impressive balance, making it a standout choice for everyday users. Here’s a deeper dive into this case’s performance, practicality, and aesthetics.
Unpacking Military-Grade Protection
The first feature that grabs your attention is the military-grade protection claim. Unlike many cases that use the term as a buzzword, Miracase delivers. The corners are reinforced with shock-absorbing material, offering a solid defense against accidental drops. I tested this (intentionally dropping the phone from about waist height), and the iPhone 15 emerged unscathed—a testament to the case's reliability. The anti-scratch back panel further ensures your phone looks pristine, even with daily use.
MagSafe Compatibility Done Right
This case integrates seamlessly with MagSafe accessories, from chargers to wallets. The magnets are powerful enough to keep your MagSafe charger locked in place but not so strong as to make removing the accessories a struggle. Charging performance remains efficient, with no noticeable slowdowns compared to using the phone without a case. This makes it ideal for users who rely on wireless charging.
Dual Screen Protectors: A Value-Added Bonus
The inclusion of two screen protectors is a thoughtful touch. These aren’t flimsy add-ons; they are well-crafted with edge-to-edge coverage and excellent clarity. Application is easy with the provided guide frame, minimizing air bubbles—a detail often overlooked by competitors.
Anti-Fingerprint and Smudge Resistance
One of the standout features is the case’s anti-fingerprint coating. Many cases promise this but fail in practice. After a week of continuous use, the Miracase still looked clean and sleek, even with sweaty hands or frequent handling. The matte finish not only resists smudges but also adds a touch of understated elegance to your device.
Slim Profile Meets Grip Security
Despite its rugged features, the case maintains a slim profile, sliding easily into pockets. The textured sides provide a secure grip, reducing the chances of accidental drops. This combination of slimness and grip is rare in protective cases and makes the Miracase ideal for on-the-go lifestyles.
What Sets It Apart?
What truly makes the Miracase Magnetic Series unique is its thoughtful design for real-world use. It doesn’t just protect your phone; it enhances your interaction with it. From its MagSafe efficiency to its smudge-resistant finish, it’s clear that Miracase designed this case with user convenience in mind.
Who Is This For?
This case is perfect for individuals who want a no-nonsense protective case that doesn’t compromise on style or functionality. It’s particularly suited for professionals, students, and anyone who appreciates the integration of thoughtful design and durable performance.
Final Verdict
At its price point, the Miracase Magnetic Series Designed for iPhone 15 is an exceptional value. With its military-grade protection, MagSafe compatibility, and added extras like quality screen protectors, it confidently holds its own in a crowded market. Whether you prioritize protection, style, or everyday convenience, this case delivers on all fronts.
Rating: 9.5/10
A near-perfect blend of style, durability, and user-focused design.
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Reviewed in the United States on January 18, 2025
★★★★★ 5
Great phone case!
Size: iPhone 16e/17e, Color: Black
Perfect protective case for my iPhone 17, without it the phone was too small and easily slipped out of my hand this makes it easier to hold but it’s not too big, it even comes with two screen protectors.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Fit great, seems sturdy
Size: iPhone 16e/17e, Color: Black
I just got it Fit perfect, seems sturdy. The screen cover was easy to install as well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 3, 2026
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