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Description
Mouse SLC30A5 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Solute Carrier Family 30, Member 5 (SLC30A5) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute Carrier Family 30, Member 5 (SLC30A5) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Solute Carrier Family 30, Member 5 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Solute carrier family 30, member 5 (SLC30A5), also known as ZnT-like transporter 1 (ZNTL1 or HZTL1) or ZNT5, is encoded by the SLC30A5 gene. This gene encodes a member of the SLC30A/ZnT family of zinc transporters. ZnT proteins mediate cellular zinc efflux and zinc sequestration into membrane-bound organelles. The encoded protein functions as a heterodimer with zinc transporter 6 in the early secretory pathway and also regulates zinc sequestration into secretory granules in pancreatic β-cells. Multiple splice variants of the transcript encoding this gene have been observed, and a pseudogene for this gene is located on the long arm of chromosome 19. It may transport zinc into β-cells for insulin crystal formation. It partially regulates cellular zinc homeostasis. It works with ZNT7 to activate zinc-requiring enzymes, alkaline phosphatases (ALPs). It delivers zinc to the lumen of the Golgi apparatus and to the vesicular compartment where ALPs reside, thereby converting apoALPs into holoALPs. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
Perfectly fit
Color: Crystal Clear
I recently purchased a Mac Neo protective case, and it has exceeded my expectations. The case fits my laptop perfectly, with precise cutouts that ensure all ports are easily accessible and well-aligned. I especially appreciate the crystal-clear design, which maintains the original look of the device while providing solid protection. The included keyboard cover is a great bonus—it feels comfortable to type on and doesn’t interfere with responsiveness. Overall, the case combines functionality, protection, and aesthetics very well.
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Reviewed in the United States on April 19, 2026
★★★★★ 5
Love this little thing. Totally worth every penny!
Color: White, Color: White
I love this little powerhouse label maker. Totally worth the value and ease of use and functionality. Also I love that I can easily change text size and font very simply while using. I have used it so many times already and I often make a label for something just because it’s easy and fun and makes things feel very organized. Helped me be very organized in my kids homeschool room, with art supplies, my storage drawer cart and my filing cabinet. Definitely worth buying and it comes with a good amount of labels. I’ve purchased another roll since buying it but I’ve labeled a ton of things. Simple, easy to do on your phone and the labels turn out perfect, clear, concise and even.
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Reviewed in the United States on March 25, 2026
★★★★★ 5
Perfect for Organization and Super Efficient
Color: White
I got this label maker because I am going to be a teacher and needed an easy way to label and organize a lot of materials, and it has been perfect for that.
It is very quick and efficient, and I was able to start using it right away without any issues. The labels come out clear, and the whole process is simple and convenient, especially when you have a lot to get done.
I also really like how compact and easy to use it is, which makes it great for both classroom and home organization.
Pros:
• Very fast and efficient
• Easy to set up and use
• Great for organizing classroom materials
• Compact and convenient
• Great value for the price
Overall:
This is a great tool if you need to label and organize quickly. I would definitely recommend it, especially for teachers or anyone who likes to stay organized.
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Reviewed in the United States on April 5, 2026
★★★★★ 5
Works wonderfully, I'm obsessed with labeling!
Color: Green
This is super easy to set up and use. I love the color and the size. I didn't want a label maker that needed a whole drawer to live in. The print is great, I love the multiple font options. My only complaint is that the label paper cutter on this actually cuts it too short. So when you go to print the next label it believes there is no paper. For the price point, I'd say absolutely worth the purchase
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Reviewed in the United States on April 21, 2026
★★★★★ 4
Good, convenient and portable printer
Color: Green, Color: Green
This printer works great for my needs. The battery life has been good and the printer app functionality has worked without any issues. For me it is very easy to use and understand so overall I think it is a good, portable and convenient printer.
However my one ad only issue is the labels get stuck and causes an error about 80% of the time meaning the label won’t print out unless I open it and realign it perfectly. That is a pain! I am contemplating returning it for a new one to see if it is just a design flaw or a printer issue so I am rating it 4 stars.
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Reviewed in the United States on April 30, 2026
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