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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.3 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Great buy, finally a big soft toy, and so cute
Color: Green
This octopus toy is so adorable, I’m truly so impressed with the size finally a big toy for a big dog. She loves it! It squeaks, soft, made great. Worth it! Love it! Fun, durable n chewability just for play materials soft n durable. It’s a sea foam green color and creme beautiful neutral color
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Reviewed in the United States on January 31, 2026
★★★★★ 5
Large, excellent toy with many squeakers!
Color: Blue, Color: Blue
This toy is excellent! I’m super happy with this purchase. My dog loves squeakers and there are so many in this toy, plus stuffing for her to rip out, and crinkle paper to take the sensory experience up another notch! It’s also more durable than many other plush-type dog toys, and is larger than expected which is a plus in my book.
For size reference, both dogs are around 40lbs.
I will be buying another as soon as this one is demolished!
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Reviewed in the United States on February 18, 2026
★★★★★ 5
Huge and fun. Good for two dogs to play with together.
Color: Yellow
This dog toy is great! And it's HUGE. Each tentacle crinkles, and the dogs love to play tug-of-war with it. It seems to be holding up well, too. It seemed a bit pricy for a dog toy, but it turns out it was worth it.
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Reviewed in the United States on January 11, 2026
★★★★★ 5
My dogs new favorite toy!
Color: Blue
His new favorite toy! My dog absolutely loves it!!! It’s much bigger than I thought it would be so my dog has to be careful when he’s running with it because he will trip on the legs of the octopus. The best part is there is a little squeaker in the bottom of each leg! Bonus!!! If your dog chews up stuffed animals, this will be no different. It is a stuffed animal. My baby just likes to carry it around and squeak it a 1000 times a day. Don’t mind my crazy voice in the video. That’s the voice I use when he’s excited about a toy. He greets me every day with his octopus.🥰
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Reviewed in the United States on February 13, 2026
★★★★★ 4
Fun while they lasted
Color: Green
I ordered two. One for each pup. Both my puppies loved this toy. The first one lasted less than a day with my pup who loves to do the death shake on everything. The second lasted two days with the other as she chews in the same spot. They loved the crinkle noise. However, I'm taking one star away due to not lasting long. Everything else was fine and they enjoyed them while they lasted. Note that most toys do not last long with my pups.
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Reviewed in the United States on May 4, 2026
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