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Description
Human IL-5 OneStep ELISA KitProduct Specification Antigen IL 5 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 4. 7% Inter assay: 6. 4% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 1. 1 pg ml Range 3. 9 pg ml 250 pg ml Recovery Cell culture supernatant 85% Serum 102% Plasma 97% Assay time 60 minutes Species
Product Specification
| Antigen | IL-5 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.7%； Inter-assay: 6.4% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.1 pg/ml |
| Range | 3.9 pg/ml - 250 pg/ml |
| Recovery | Cell culture supernatant 85% Serum 102% Plasma 97% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | The dose response curve of the International Standard (90/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (90/586) units, use the following equation: NIBSC (90/586) approximate value (IU/mL) = 0.01 x OneStep Human IL-5 value (pg/mL). |
Background
IL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.
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