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Description
CD20 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-R133)Product Specification Host Rabbit Antigen CD20 Synonyms B lymphocyte antigen CD20, Bp35, Leukocyte surface antigen Leu 16, MS4A1 Location Cell membrane Accession P11836 Clone Number S R133 Antibody Type Recombinant mAb Isotype IgG Application ICC, IF Reactivity Hu Purification Protein A Concentration 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 0. 1% BSA, 0. 01% Proclin 300 Stability & Storage 12 months from date
Product Specification
| Host | Rabbit |
| Antigen | CD20 |
| Synonyms | B-lymphocyte antigen CD20, Bp35, Leukocyte surface antigen Leu-16, MS4A1 |
| Location | Cell membrane |
| Accession | P11836 |
| Clone Number | S-R133 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | null |
| IF | 1:200 | null |
Background
The CD20 antigen is a membrane-embedded, non-glycosylated phosphoprotein, 33-37 kDa. CD20 functions as a Ca2+-permeable cation channel, involved in the regulation of B-cell activation, proliferation and differentiation. CD20 appears on the surface of the pre-B lymphocyte between the time of light chain rearrangement and expression of intact surface immunoglobulin and is lost just before terminal B-cell differentiation into plasma cells. Surface expression of CD20 on activated B cells is approximately 4-fold greater than that found on resting B cells. CD20 is virtually specific for normal B-cells. A weak expression has been demonstrated in a subpopulation of T-cells, but not in any other cell type [NordiQC].
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