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Description
Caspase 6 Activity Assay KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 6 enzyme activity: 1. Take out an appropriate amount of Ac-VEID-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VEID-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VEID-pNA (2mM). Add Ac-VEID-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 6 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 6, it can catalyze the substrate Val Glu Ile Asp p nitroanilide (Ac VEID pNA) to produce yellow pNA (p-nitroanilide), which can be used to detect the activity of Caspase 6 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 6 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader, spectrophotometer |
| Synonym | Mch-2,caspase-6,caspase 6 |
| Description | caspase 6 Activity Detection Kit is a kit that detects caspase 6 enzyme activity or purified caspase 6 enzyme activity in cell or tissue lysates by spectrophotometry. |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Durable Toy for Active Dogs
This ball is strong enough to handle daily play while keeping dogs entertained. It provides stimulation that helps reduce boredom and restlessness. The material feels tough but safe for dogs, and the interactive design makes it more exciting than a regular ball. It’s also lightweight enough for indoor or outdoor use. A smart choice for pet owners looking to keep dogs occupied.
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Reviewed in the United States on November 13, 2025
★★★★★ 5
Bright, easy to clean toy
This ball is a fun little addition for my big dog. The bright blue color really helps, it is easy to spot under the sofa or behind furniture. The outer shell feels durable, not soft rubber but springy enough to handle his chewing without marks. When switched on, it rolls around by itself and makes my dog chase it all over the living room, which is a good way to burn his energy on rainy days. The internal switch is hidden, which I like because my dog cannot open it with his teeth. It is simple to turn on and off, and so far, the movement works as described. The texture also resists hair sticking, and I can rinse it under water, and it looks clean again in seconds. For the price, it serves its purpose and gives my dog a short burst of excitement each day.
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Reviewed in the United States on October 10, 2025
★★★★★ 5
Fun and Tough Interactive Toy for My Big Dog
This interactive dog ball has been a great addition for my large dog. The ball’s material is very durable and holds up well even with heavy chewing. My dog usually destroys toys within days, but this one has lasted weeks and still looks great. The automatic play modes keep him active and entertained, especially when I’m busy. He loves chasing it both indoors and outdoors, and it helps release his energy. The battery life is decent, and the operation is simple. Overall, it’s a fun, sturdy, and smart toy that really keeps my dog busy and happy
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Reviewed in the United States on October 11, 2025
★★★★★ 5
Finally, a Toy That Keeps My Dog Entertained and Active
I bought this interactive dog ball for my German Shepherd, who is very energetic and can be quite aggressive with his chewing. I've tried countless toys that either failed to hold his attention or broke within a week, but this ball is incredibly durable. The durable E-TPU material feels tough and flexible, yet lightweight, making it easy for him to carry around. The bright blue color makes it easily recognizable indoors or out, and I can spot it at a glance.
The ball bounces and rolls in unpredictable patterns, keeping him alert and focused. When he tires of chasing, I switch it to slow mode so he can push the ball around easily. I've noticed he's calmer after playing in the evenings.
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Reviewed in the United States on September 27, 2025
★★★★★ 5
Great way to keep my dog entertained!
My German Shepherd gets bored easily and tends to chew through most toys, but this interactive ball has been a lifesaver. The durable E-TPU material really holds up against his strong jaws. I love that it has 3 different play modes – it keeps him curious and busy for hours, both inside the house and in the backyard. A perfect mix of durability and fun!
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Reviewed in the United States on September 26, 2025