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Description
mIHC Dye (TSA520)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.6 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 4
Second-chance, slow-burn sapphic romance
Format: Paperback
"Firecamp" by Jaycie Morrison is a second-chance sapphic romance that includes an emotionally-charged slow burn romance and a time-jump.
MCs Nora and Fallon come from very different circumstances, but are thrown together along with six other women at Firecamp where they all must work to help mitigate wildfires. Both emotionally immature and carrying their own baggage, Nora and Fallon's young romance ends with a bang. Ten years later, with their careers leading the way, they are forced back together. What follows is a slow burn, second-chance romance that is less naive but no less passionate.
This was my first book by Jaycie Morrison, but it definitely won't be my last. I really enjoyed the way she developed Nora & Fallon independently and together as well as the "side action" that we saw with Nora's family, especially her younger brother Tyler. I was initially drawn to the story because it was set in the Colorado wildfire scene & I loved how that was portrayed.
Thank you NetGalley and Bold Strokes Books for the eARC.
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Reviewed in the United States on May 19, 2025
★★★★★ 3
Had high hopes, but didn't hit the mark
Format: Paperback
I wanted to like this one a lot more than I did. A sapphic romance set in the wilderness doing fire mitigation is right up my alley, but Firecamp just didn't hit the mark for me. The pacing was clunky, and ultimately I was left frustrated wondering when it would end. The author went into way too much detail chronicling the itty bitty events, and didn't spend nearly enough time on the MCs actual personal growth or relationship. It often felt like "well they're together because they're supposed to be."
All in all, I feel bad leaving a negative review because we need more sapphic stories BUT I do feel like this could have benefitted from some more story-specific editing, not just grammar/spelling.
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Reviewed in the United States on May 26, 2025
★★★★★ 5
The Colombo Family Duet
Format: Kindle
Another amazing mafia romance duet!
Widowed Brie "Breezy" Colombo teams up with a reluctant Dominika "Nik" Kusek to try and solve her husband's murder. Nik is tasked to be Brie's bodyguard as they dodge hit men, play in mafia politics, and look for any clues that could lead them to a killer.
There was action, mystery, loyalty, betrayal, passion, lots of steam... it was all around awesome! I breezed through both books in one day!
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Reviewed in the United States on August 21, 2025
★★★★★ 4
Great read!
Format: Kindle
This one takes you on a ride from start to finish. Another great sapphic mafia romance by Persephone!
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Reviewed in the United States on April 7, 2025
★★★★★ 5
Great Story.
Format: Kindle
I really enjoyed the none stop action and the thrilling suspense of who killed Terry Colombo as well as who put out hits to kill Brie. Also liked the simmering romance between Brie and Dominika "Nik" Kusek. I was little disappointed that there were no specific detail of there height just vague description. Though it did not change my opinion of this story, or the number of stars given. I found this story very entertaining and exciting to read. I can't wait to read what happens next in book 2. I whole heartily recommend this story to everyone that loves a little danger with their romance.
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Reviewed in the United States on November 9, 2024
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