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Description
Human Galectin 3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Galectin 3 capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Galectin 3 in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Galectin 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Galectin-3 is a protein encoded by the gene LGALS3 located at positions q21-q22 on chromosome 14 and is a member of the lectin family. Galectin-3 is approximately 30 kDa and, like all galectins, contains a carbohydrate recognition domain (CRD) of approximately 130 amino acids that specifically binds to β-galactose. Galectin-3 (Gal-3), also a member of the β-galactoside-binding protein family, plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, and apoptosis. Galectin-3 is expressed in the nucleus, cytoplasm, mitochondria, cell surface, and extracellular space. It has an affinity for β-galactose and exhibits antimicrobial activity against bacteria and fungi. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.0 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 4
LOVED Tucker!!
Format: Kindle
4 stars — I was actually most looking forward to Tucker’s story, and while I loved it, it didn’t end up being my favourite. Weird how that works right?
Now as I fully anticipated, I LOVED Tucker. Like LOVE LOVED him. He was everything I was hoping for and more. I adored how he was this delicious blend of sweet, caring, genuinely good guy mixed with a delightfully dirty mind. I think that was the part that surprised me, though I’m not sure why. But damn, that boy had it going on!! And yet he was still so gentlemanly…god, I love that mix. And he was so freaking patient! Like, I couldn’t even believe it sometimes. He was almost too patient on occasion, b/c he wouldn’t push Sabrina at all, and maybe she needed a little push. But I loved how he could see through Sabrina’s bullcrap to the heart of her. And I loved how he didn’t let the curveballs throw him off his path, he stayed true to himself and wouldn’t make choices that he couldn’t be happy with when it came to his life.
While I wasn’t surprised that I loved Tucker, I will admit that I was surprised I loved Sabrina too. I loved how driven she was, and how she put on that persona of being a witch with a b to keep people away, but underneath she was extremely vulnerable. I also thought that Ms. Kennedy did a great job of showcasing the challenges of poverty through her situation. She desperately wanted a better life, and she thought she knew exactly what that better life would look like. While both Sabrina and Tucker aggravated me with their stubbornness and wrong assumptions (it’s not my favourite trope), she took a bit longer than I wanted to figure stuff out. It’s not that I didn’t get that her family life and childhood damaged her, but she was being an idiot and I was sad that none of her friends woke her up. Another thing that bugged me was that, in my opinion, she was seriously emotionally abused, and I kind of wished that that had been addressed at some point. Her Nana was, quite frankly, awful to her, and her love felt very conditional. And the way Sabrina would excuse Ray’s behavior…well, I just wish that someone had told her that that’s not normal, and gotten her some guidance.
Tucker and Sabrina were interesting together. Obviously they had amazing chemistry, and there were so many sweet moments that I just loved. But their relationship was a bit dysfunctional, and I felt like I needed a bit more near the end when things changed. I just would have appreciated learning a bit more about their motivations, or seeing more frank discussions between the two of them…it just felt like I didn’t understand why this time it was different, you know?
The plot in this one flowed a bit differently for me, especially the second half. It just didn’t feel like the usual narrative structure I’m used to, with the build up to a conflict and climax. As I said, I kept waiting for the turnaround, but it just kind of snuck in there with a lot of little ups and downs.
And can I also say that I hated the way Tucker’s Mom behaved? She was truly awful, and I’m not sure I ever fully understood her motivations, or what she was like, or how Tucker became who he was with that kind of mother.
On the completely other side of it, I loved seeing the group interact again. I really love all those boys and the girls they fell in love with. I also really appreciated that Dean and Sabrina worked things out, but weren’t all buddy buddy. And as for other secondary characters, I loved Sabrina’s friends and seeing a bit more of some of the other hockey boys. I’m so excited to know we’ll be getting a bit more of some of them.
So yeah, so much goodness in this one, just a few little niggles that made it not the hit out of the park I was expecting. But a seriously great end to a fantastic sports romance series.
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Reviewed in the United States on March 9, 2018
★★★★★ 3
John Tucker made this book
Format: Kindle
The Goal (Off Campus #4) by Elle Kennedy
3 stars!!
“I’m not the girl for John Tucker, and I never will be.”
After the bomb was dropped at the end of The Score I was more than eager for John Tucker’s story, he was a character that had always blended into the background and we never really got to know him throughout the other books in this series, but as they say, the quiet ones are often the worst.
However, John Tucker was adorable in every sense of the word. He really surprised me in The Goal. He was one of the most loyal and loving guys out of all of them and had the patience of a saint to back it up and with Sabrina James he certainly needed it. But also, Tucker was as sexy as hell and had a filthy mouth, I never would have guessed it. For some godforsaken reason Tucker loved Sabrina, whereas some guys would have given up and run for the hills, Tucker was glutton for punishment, he took the punches, he took the rejection, but would he get a happy ever after?
“Even if you hadn’t said you loved me back, I’d take whatever scraps you were willing to give me as long as I could be with you. I don’t give a s**t if that makes me pathetic-”
Sabrina James, she was one cool customer who I just couldn’t warm up to. I admired her drive and determination, her focus on bettering herself but her treatment of Tucker just wound me up no end. She was the puppet master and she definitely pulled all the strings and led our Tucker on a merry dance. Her coolness and aloofness throughout just grated on my every last nerve. If Tucker was insincere I could understand it, but she knew deep down that she held Tucker’s heart in her hands and had no qualms about toying with his emotions.
“It doesn’t matter how thin or thick anyone’s wallet is. We all hurt. We all love. We’re the same. And your past, who you live with, where you came from, it doesn’t have to matter. You’re creating your own future, and I want to see where the road forward takes you.”
For me though, my biggest gripe with this book was pacing. This story runs parallel with The Score and so a chunk of the plot line was repetitive. I just felt that as situations were rehashed through someone else’s eyes it lost its impact and for me interrupted my reading mojo. If you are reading this as a standalone and have not read The Score, then this shouldn’t be an issue. The first half of the book was particularly slow for me, however, as everything hots up in the second half it pulled me back in.
“My goal, once upon a time, was to succeed. I didn’t realize that success wasn’t grades or scholarships or achievements, but the people I was lucky enough to have in my life.”
My heart definitely belonged to John Tucker in this book, this guy had a heart of gold, was the most loving and giving, he gave Sabrina everything she wanted and needed and yet she still kept him at arm’s length. He was forever trying to bore little holes into her life and heart to inch that little bit forward but she was an emotional fortress, it all seemed a little one sided. They get there in the end but she was definitely a tough nut to crack.
“I can’t make a single decision. Not until Sabrina makes the most important one of all.”
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Reviewed in the United States on September 27, 2016
★★★★★ 5
Let's go!
Format: Hardcover
This book is a classic! We purchased for our graduating senior and had all of her former teachers and coaches sign it with a special message on the pages. Great idea for a wedding or baby shower too. The quality is great! Love Dr. Seuss!
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Reviewed in the United States on May 2, 2026
★★★★★ 5
A Great Graduation Gift
Format: Hardcover
This was one of my favorite books when I was a child. I've been purchasing this as a graduation gift for years. I gave it to both my daughters when they graduated high school and was happy to read that very copy we gifted our daughter to my granddaughter. The book has also helped me in some difficult times of my own life. Dr. Seuss is timeless and this makes a great gift.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
The Perfect Gift for a New Beginning
Format: Hardcover
Oh, the Places You'll Go! by Dr. Seuss is a timeless and encouraging read filled with humor, wisdom, and inspiration about life’s journey and all of its ups and downs. This is such a meaningful gift for a high school senior, graduate, or anyone stepping into a new chapter of life.
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Reviewed in the United States on May 7, 2026