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Description
Human Killer cell lectin-like receptor subfamily G member 1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Killer cell lectin-like receptor subfamily G member 1. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the presence of Killer cell lectin-like receptor subfamily G member 1 in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Killer cell lectin-like receptor subfamily G member 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Killer cell lectin-like receptor subfamily G member 1 is a protein encoded by the KLRG1 gene. KLRG1 is a lymphocyte co-inhibitory or immune checkpoint receptor primarily expressed on late-differentiated effector and effector memory CD8+ T and NK cells. Its ligands, with similar affinities, are E-cadherin and N-cadherin, markers of epithelial and mesenchymal cells, respectively. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.8 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Kids love the newest “Worst” tale-
Format: Hardcover
Kids will love this next book in The Worst series. Awesome, fun, colorful illustrations in this tale with Gilbert the Goblin trying to escape cupids and valentines. Instead of candy for Valentine’s Day give the gift of this book. My students love when Alex Willan has a new book out and are loving this one.
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Reviewed in the United States on January 17, 2026
★★★★★ 4
Plenty of fun for Gilbert fans!
Format: Hardcover
As in previous books in the series, Gilbert the Goblin complains about the problems he sees with Valentine's Day, what he calls 'mushy malarkey'. Young readers who don't care for the holiday will find much to relate to in the book. Although, Gilbert's complaints seem a tad trifling. Coconut chocolates are 'Blech'. He seems to have a special issue with the cupid's 'perfect', 'beautiful', and 'luxurious' hair. When Gilbert faces off with a group of cupids, he just barely manages to escape their love arrows, or so he thinks until he backs into a cactus in his own yard and believes he has been pricked by one such love arrow. He then becomes a goblin full of love. He loves his spells, his potions, even himself, but not coconut chocolates. He even welcomes his friends over to celebrate before bidding his pet sock slugs a fond goodnight. Good thing the love spell wears off. This funny book follows the pattern of the previous five books, but the addition of adorable cupids as well as Gilbert's beloved cactuses and pet sock slugs provides plenty of freshness as Gilbert once again (reluctantly) joins in the festivities. Plenty of fun for young fans of the series.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 10, 2026
★★★★★ 5
LOVE this book! ❤️
Format: Hardcover
I am a kindergarten teacher and my students LOVE this series! Valentines are the Worst is a new favorite! The illustrations are amazing as always and the story is funny and sweet, perfect for Valentine's Day!
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Reviewed in the United States on January 17, 2026
★★★★★ 5
The best
Format: Hardcover
One of the best in this series! All kids will love it❤️
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 17, 2026
★★★★★ 5
Best way to explore Paris
Format: Paperback
This book is awesome! I enjoyed reading it just as much as my kids did! I love the layout and the way that the book assigns the kids to be secret agents, but defers to the case officers (parents) for the final say. We were in Paris for 1 week and although the weather was less than cooperative, we were able to capture our 100 points and award our 4yo and 7yo their secret agent certificates. I love the fact that you can work on this book in any order and do as little or as much as you want at each attraction. The price was awesome and worth every penny. My 7yo particularly enjoyed the challenges at the Musee D'Orsay and the Louvre and it was a great way to keep those visits focused and entertaining for the kids. My 4yo was a little young to stay engaged with every "mission", but the activities were easy enough that she could help (ex. finding a winter scene in the Musee D'Orsay). I loved that this book engaged the kids but didn't detract from any of our visits (we didn't feel like we had to go "out of our way" to find anything). The only disappointment was that they didn't include Versailles and Sainte Chappelle. I have not only recommended this book to all my of friends traveling to Paris (honestly with or without kids), but I have also already purchased the similar books for London, New York, and Washington DC. Can't wait to see what other cities they add!
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Reviewed in the United States on June 28, 2016
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