SKU: 51920326583

UA-Glo® Fluorescent Cell Viability Assay

Sale price$113.40 Regular price$126.00
Save 10%

Pay in installments of $31.50 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 20 - Aug 25

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

UA-Glo® Fluorescent Cell Viability AssayProduct Specification Synonyms Fluorescent Cell Viability Assay Kit Stability & Storage Store at 20 or below. The validity period is 12 months. Background There are various methods for cell viability detection, such as detecting dye exclusion, changes in ATP concentration, and changes in enzyme activity. Cell detection sometimes requires research on the mechanism of cytotoxicity and or the establishment of internal reference controls, which gives rise

Product Specification


Synonyms Fluorescent Cell Viability Assay Kit
Stability & Storage

Store at -20℃ or below. The validity period is 12 months.

Background

There are various methods for cell viability detection, such as detecting dye exclusion, changes in ATP concentration, and changes in enzyme activity. Cell detection sometimes requires research on the mechanism of cytotoxicity and/or the establishment of internal reference controls, which gives rise to the need for multiplex analysis. That is, the same sample is detected using multiple analytical methods with different detection mechanisms that do not interfere with each other. This allows for relevant comparisons to reveal the mechanism of cytotoxicity or exclude system differences. The detection of cell viability by our company's Fluorescent Cell Viability Assay Kit (UA-Glo® Fluorescent Cell Viability Assay) is based on the fact that proteases present only in living cells degrade peptide-fluorophore substrates to produce fluorescent signals. When the integrity of the cell membrane is impaired, these proteases lose their activity and no fluorescent signals are generated. The UA-Glo® Fluorescent Cell Viability Assay has a different detection principle from our company's UA-Glo® Luminescent Cell Viability Assay (Catalog No. UA070103) and can detect milder cell damage at an earlier stage. The two assays are compatible for multiplex detection. The UA-Glo® Fluorescent Cell Viability Assay is also compatible with our company's UA-Glo® Caspases 3/7 Cell Apoptosis Detection Kit (Catalog No. UA079012), enabling multiplex detection to determine the mechanism of cytotoxicity.

Components

Protease substrates and buffer solutions are mixed and filled into 10 ml or 100 ml brown bottles and 2 ml tubes. The specifications are as follows:

specifications

CTF substrate

CTF buffer

Detectable number of wells in 96-well plate

Detectable number of wells in 384-well plates

10 ml

100μl

10ml

100 wells

500 wells

5X10ml

5x100μl

5x10ml

500 wells

2,500 wells

2X50 ml

2x500μl

2x50ml

1,000 wells

5,000 wells


Protocol

1. Cell Preparation
1) Seed the cells to be tested at an appropriate density in a 96-well or 384-well cell culture plate. It is recommended to use a black-framed transparent cell culture plate.
2) Add the test compound at an appropriate concentration to the wells of the cell plate. The concentration of organic solvent in the culture medium should be kept below 1%. Continue culturing for an appropriate time according to the experimental requirements.

2. Cell Viability Assay
1) Take out the fluorescent cell viability assay reagent. After the CTF buffer is completely dissolved, equilibrate it to room temperature. Vortex the CTF substrate to mix it thoroughly, and centrifuge briefly to collect the CTF substrate at the bottom of the tube.
2) Prepare the CTF reaction solution: The CTF substrate is 100x. Prepare a 1x reaction solution with CTF buffer according to the required amount, and mix well.
3) Take out the cell culture plate to be tested, and add an equal volume of CTF reaction solution to each well. For example, add 100 μl of 1x CTF reaction solution to 100 μl of culture medium.
4) Shake the plate at low speed for 20 seconds to mix, and perform fluorescence detection after reacting at 37°C in the dark for 60 minutes. Different cells have different cleavage rates for the CTF substrate, so the optimal plate reading time needs to be optimized according to the cells. Plate reading can be done as early as 30 minutes after adding the reagent. Generally, the signal-to-noise ratio of the results read 60 minutes or longer is higher. It is recommended that the maximum plate reading time does not exceed 3 hours.
5) Read the fluorescence signal on a fluorescence microplate reader with excitation light (Ex) of 380-400 nm and emission light (Em) of 505 nm.
6) If needed, proceed with downstream multiplex analysis, such as Caspases 3/7 cell apoptosis detection.

Guidelines

1. It is recommended to aliquot the substrate and store it at -20°C after initial use, and prepare the reaction solution freshly each time.
2. It is not recommended to arbitrarily change the amount of reaction reagents without strict verification.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 51920326583

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.7 ★★★★★
Based on 10 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
A
Verified Purchase
Amazon Customer
Boise, US
★★★★★ 4
it's ok
Size: 7 Pcs Bathroom Set, Color: Green
Pretty color and size. Took off a star because the plastic looks on the cheap side, but it's ok
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 18, 2025
N
Verified Purchase
Norma Torres
Belleville, US
★★★★★ 5
Very nice
This sets is good, the size is perfect,very well made not that cheap plastic. It looks great in my bathroom and color matches great with my shower curtain and rugs. I wonder if they come in other colors.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 9, 2026
B
Verified Purchase
ben johnson
Lake Worth, US
★★★★★ 3
Cheap plastic
Size: 7 Pcs Bathroom Set, Color: Grey
Was what I ordered,but the handle for the toilet brush broke on second use. Dollar store brushes are better than this one.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 2, 2026
C
Verified Purchase
Chillicothe30
Boise, US
★★★★★ 5
Cost
Size: 7 Pcs Bathroom Set, Color: Green
Very nice
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 12, 2026
N
Verified Purchase
Néstor ramirez
Omaha, US
★★★★★ 5
Recomendado
Muy bueno lo recomiendo le doy 5 estrellas
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 24, 2026

recommand products