PLCγ2 Recombinant Rabbit mAb (S-2749-14)

PLCγ2 (Phospholipase C gamma-2) is a 1,565-residue signaling enzyme that harbors an N-terminal SH2-SH3-SH2 cassette, a split PH domain, tandem EF hands, a catalytic Y-domain TIM barrel, and a C-terminal tail containing two phosphorylatable tyrosines (Y753/Y759); upon recruitment to phosphorylated ITAM-bearing adaptors (e.g., DAP12, FcRγ) or receptor tyrosine kinases, Syk/BTK family kinases trans-phosphorylate these tyrosines, triggering allosteric relief of an auto-inhibited conformation that enables the lipase to hydrolyze PIP2 into IP3 and DAG, thereby mobilizing Ca²⁺ and activating PKC, while simultaneously serving as a scaffold for assembling signalosomes that drive cytoskeletal remodeling, ROS generation and transcriptional programs essential for osteoclastogenesis, B-cell development, platelet aggregation, and innate immune responses; gain-of-function mutations (e.g., S707F, R665W) produce constitutive PIP2 cleavage and cause immune-dysregulatory diseases such as PLAID, APLAID and cold urticaria, whereas loss-of-function variants underlie antibody deficiency and osteopetrosis, making PLCγ2 a druggable node in inflammation, autoimmunity, and hematologic malignancies.