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Description
UniOne® TR-FRET Human BRD4/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the Molecular Glue BET Degrader 1 and testcompound mediated interaction between the DDB1 CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh throughput format for detecting small molecules capable of mediating theinteraction between the DDB1 CRBN
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the Molecular Glue BET Degrader-1 and testcompound-mediated interaction between the DDB1/CRBN complex and BRD4. BRD4contains bromodomain 1 and 2. This method enables a simple and rapidhigh-throughput format for detecting small molecules capable of mediating theinteraction between the DDB1/CRBN complex and BRD4.
As shown, the interaction between DDB1/CRBN and BRD4 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the Molecular GlueBET Degrader-1 mediates the interaction between DDB1/CRBN and BRD4, the donorand acceptor antibodies are brought into proximity. Excitation of the donor antibodytriggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665nm. This specificsignal is proportional to the degree of interaction mediated by BET Degrader-1between DDB1/CRBN and BRD4. This homogeneous assay is simple to perform andrequires no washing steps.
Components
Component |
Concentration |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|
Tag1-DDB1/CRBN protein |
25× |
80μL |
400μL |
1600μL |
-80°C |
Tag2-BRD4 protein |
100× |
20μL |
100μL |
400μL |
-80°C |
BET Degrader-1 (100μM) |
10μM |
10μL |
50μL |
200μL |
-80°C |
Anti-Tag1 Eu dye antibody |
50× |
50μL |
250μL |
1000μL |
-80°C |
Anti-Tag2 Ac antibody |
12.5× |
200μL |
1000μL |
4000μL |
-80°C |
Detection buffer |
10× |
2mL |
10mL |
40mL |
-80°C |
*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
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