Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 7 - Aug 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human SBF2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a SET binding factor 2 (SBF2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of SET binding factor 2 (SBF2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human SET binding factor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | SET binding factor 2 (SBF2) is a protein encoded by the SBF2 gene. Disorders associated with SBF2 include Charcot-Marie-Tooth disease type 4B2 and neuropathy, congenital hypomyelination 1, autosomal recessive. Pathways associated with SBF2 include intracellular trafficking of proteins involved in CMT neuropathy and phosphatidylinositol metabolism. Gene Ontology (GO) annotations associated with this gene include protein homodimer activity and phosphatidylinositol binding. An important homologue of this gene is SBF1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.7 ★★★★★
Based on 16 reviews
Sort
Product Reviews
★★★★★ 5
My Dogs' Absolute Favorite Chew: Tough, Flavorful, and a Lifesaver for Boredom!
What sets the Benebone Wishbone apart isn't just its durability—though that's impressive enough—it's the thoughtful design that caters to how dogs actually play and chew. Crafted from super-tough nylon infused with real bacon flavor (not some artificial nonsense that fades after one gnaw), this wishbone-shaped toy is ergonomically curved for easy gripping with paws or mouth, making it perfect for aggressive chewers who like to toss, carry, and battle it around. The three prongs give them multiple angles to attack, which keeps them engaged for hours without getting bored. For my Goldendoodles and Golden Retriever, who are all large-breed goofballs with endless energy, the large size (about 7.5 inches long) is spot-on—not too big to intimidate, but substantial enough to withstand their enthusiastic chomping. We've had ours for over two months now, and while it's showing some honorable battle scars (tiny grooves from their teeth, which actually make it easier for them to grip), it's nowhere near splintering or breaking. Compare that to the Nylabones we've tried, which either splintered too quickly or lacked any real appeal—these Benebones win hands down every time.Flavor is where this toy really shines, though. That real bacon infusion isn't just a gimmick; it's embedded throughout the nylon, so even as they chew deeper, the savory scent and taste keep coming. The more food-motivated Goldendoodle goes absolutely feral for it—drool flying as he parades it around the living room like a trophy, grumbling possessively if the others get too close. The female Goldendoodle prefers a more playful approach, flinging it across the room before pouncing back in for wrestling matches that leave her curls even more disheveled, while the Golden Retriever settles on the rug for long, focused sessions that help him unwind after fetch marathons. It's hilarious to watch them "fight" over it (in the gentlest, most sibling-rivalry way), but what's even better is how it redirects their energy. No more shredded couch cushions or nipped at ankles during zoomies—these bones have cut down on destructive behavior by at least 80%, giving me more time to relax and less time playing fetch with toy remnants.Safety and quality are top-notch too, which is crucial when you're dealing with heavy chewers. Made in the USA with non-toxic materials, it's free from BPA, phthalates, and all that sketchy stuff you worry about with imported plastics. The company even encourages responsible use: supervise play, especially for puppies or super-aggressive types, and replace when the ends wear down (though ours are still going strong). At this price point for a toy that lasts weeks instead of days, it's an absolute steal—I've already ordered backups in peanut butter flavor to rotate in and keep things fresh.If your dogs are anything like mine—energetic, mouthy, and in constant need of occupation—this Benebone Wishbone will be a game-changer. It's not just a chew toy; it's a boredom buster, a dental helper (those ridges get the tartar going), and a source of pure joy for your furry family members. My dogs love these bones more than their walks some days, and that's saying something! Five stars all the way—highly recommend grabbing one (or three) before your next Amazon order. Your sanity (and your furniture) will thank you.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 20, 2025
★★★★★ 5
Truly Durable - No Joke, These Actually Last
I purchase these for the rescue I support, and they live up to their “durable” claim - no joke, these truly last.
Durability:
These are genuinely built for aggressive chewers. While the edges do eventually get rough with heavy use, the fact that they last a while with determined chewers is impressive. Most toys don’t make it that far.
Design:
The wishbone shape gives dogs multiple angles to chew from, which keeps them engaged longer. The ergonomic design works well for powerful chewers.
Made in USA:
Knowing these are made in the USA gives me confidence in their quality and safety, whether for rescue dogs or personal use.
Flavor:
The real bacon flavor keeps dogs interested and coming back to the toy repeatedly.
Pros:
∙ Truly durable - actually lasts
∙ Made in USA
∙ Real bacon flavor dogs love
∙ Wishbone shape for multiple grip points
∙ Perfect for aggressive chewers
∙ Edges get rough eventually but toy holds up
∙ Rescue regularly uses these
∙ Better longevity than most chew toys
Bottom Line:
When a chew toy genuinely lasts with aggressive chewers, that’s noteworthy. Yes, the edges get rough with extensive use, but the toy itself holds together far longer than most. Excellent for rescues, shelters, or anyone with power-chewing dogs. Highly recommended!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 19, 2025
★★★★★ 5
Great Bone for aggressive chewers but watch the wear & tear
I’m extremely happy I found Benebone Bacon Flavor Large Wishbone for my puppy who is an aggressive chewer. As the packaging reads this is a durable nylon. My puppy chewed the bone down to what you see in the picture within 3 days. It’s easy for him to handle. He loves the bacon flavor. He immediately took to it as soon as I gave it to him & off he went to work. I did check with my Veterinarian first to inquire if this was a safe bone & she assured me it was. She did advise to be aware of the wear & tear on the bone & toss when I felt it was time. I honestly wouldn’t of known when. I’m happy I took the time to read the inside of the packaging this bone came in. It referred me to the benebone website which showed pictures of bones-which I attached- that reflect the wear & tear & when it’s time to toss. There are the 3 categories of “Light Wear,” Moderate Wear“ & “Heavy Wear.” You’ll see the written description next to each. This I found very helpful, therefore I wanted to include it in my review, in hope of helping you. By the looks of my current bone it’s in between moderate & heavy wear. The large is costly, I feel, but it 100% occupies my puppy. It’s his favorite “toy.” I’ve only had small shavings come off since he’s chewed it. Nothing major to clean off the couch where he lays. Stuffed toys are a thing of the past now. I’ll continue to buy once it’s time to toss this bone because he loves it so much. I also love that Benebone is Made in the U.S.A.! I definitely recommend! A heads up! If you click on their store on the main page of this bone you’ll find other shapes of the nylon bone as this one. This Benebone won’t disappoint. Take the time to read the packaging. It’s informative & cool!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 7, 2023
★★★★★ 5
Very durable and long-lasting, great for strong chewers, gets rid of plaque in older dogs
I have a seven-year-old golden retriever who was never a strong chewer, she liked gnawing on toys and dental “bones” and destroyed shoes and a carpet when she was a puppy but it was never her favorite activity. Then I adopted two puppies of indeterminate breed, but likely Plott Hound and Lab, and they are aggressive chewers and want to chew on everything. I tried all kinds of teething things, as teething seemed to bother them more than any of the other four dogs I’ve had, and all were destroyed quickly, like they were treats instead of supposedly “long-lasting” chew bones. I tried these bones after I happened upon them on Amazon, and I could not be happier. My dogs contentedly gnaw on these for extended periods of time, obviously in heaven as they do so, and sometimes it’s the only way I have time to get anything done. When they were teething these definitely helped them with teething pain, and now they still want to chew on them every day. They can hold them easily and sometimes the puppies even share a bone due to the unique shape. My Golden had lots of plaque and nasty buildup on her teeth and was headed for needing dental cleanings under anesthesia, but now her teeth are sparkling white without a speck of plaque thanks to these. The bones have worn down over the course of eight weeks, but very gradually and not ever in pieces. Yes, the ends become prickly and are definitely not fun to step on, but I don’t let them chew on them outside to reduce them collecting dirt and rinse them every few days to get rid of whatever is on there. I have watched carefully for any signs of irritation in my dogs’ mouths or teeth issues, and have never seen any blood on the bones even after my dogs have determinedly chewed on them for an hour. They LOVE them, and because they are the only bones I have found that last longer than five minutes for my aggressive chewers, hold their attention for an extended period of time, and get rid of all the plaque on my older dog’s teeth, I HIGHLY recommend them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 26, 2021
★★★★★ 4
Long lasting
Works great they last a long time my dog love them. We have three of them. Always getting them out to play with. Great product for the price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 13, 2026