Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 12 - Aug 17
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human MFN2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Mitofusin 2 (MFN2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Mitofusin 2 (MFN2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Mitofusin 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Mitofusin 2 (MFN2) is a protein encoded by the MFN2 gene. Mitofusins are GTPases that are embedded in the outer mitochondrial membrane. In mammals, MFN1 and MFN2 are essential for mitochondrial fusion. It is a mitochondrial membrane protein that plays a central role in regulating mitochondrial fusion and cellular metabolism. More specifically, MFN2 is a dynamin-like GTPase that is embedded in the outer mitochondrial membrane (OMM), thereby influencing mitochondrial dynamics, distribution, quality control, and function. In addition to MFN2, OPA1 regulates inner mitochondrial membrane fusion, MFN1 mediates mitochondrial fusion, and DRP1 is responsible for mitochondrial fission. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 30 reviews
Sort
Product Reviews
★★★★★ 5
Love and Beware
This is a fabulous product but be aware that if you wash you face vigorously and splash ANY oil on your walls it will stain. I love it but hate the spots that I sometimes get. I have tried a little diluted dish soap but it doesn’t really help.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
★★★★★ 4
Whoa- I can’t believe this!
I wanted to try this product for a very specific purpose, I have been looking at facial cleansing wipes to use occasionally at bedtime instead of stripping my face by doing a full wash with cleanser and water. I rarely wear makeup, I spend my energy on finding the best skincare products for my skin and sunscreen. So at bedtime usually, I’m just taking off sunscreen and the day’s grime, or if I didn’t go out, the day’s moisturizing products. I tried several brands of moist facial cleansing wipes, and they left behind a greasy film and my face still feels like it needs a full wash. I would never want to skip properly cleansing my skin, and I usually do a full routine while I shower, but it would be nice to be able to occasionally do something quicker and more convenient without sacrificing on quality. I was hoping this product might cleanse well without needing to be rinsed or breaking me out on occasions where I want something quick and convenient, or maybe am travelling and need a basic routine.
Wow, this product is not only perfect for that, it actually makes my skin soft and clean, you’d never know I skipped my regular cleansing and just used this Bioderma product with a cotton pad before my nighttime moisturizers/serum. It completely takes off the day’s water resistant facial sunscreen and any sweat or grime and leaves my skin very clean, soft, not dry, and it feels like there is no residue whatsoever. I also don’t break out or get clogged pores, it is just a clean, soft texture. One cotton pad with product is all takes for my entire face to look and feel just as clean as if I had used my regular products (a cleansing balm followed by a foaming cleanser and lots of rinsing with water).
I have used this micellar water quite a bit since receiving it, and have not broken out or had clogged pores from “cutting corners” so to speak. And it cleanses very well. I can’t believe how well it cleanses without needing to be rinsed, without irritating my skin, without clogging pores or causing breakouts, and is suitable to use and then put on my serum and nighttime moisturizer. I’ve even used it the next morning to remove the nighttime moisturizers and then put on my SPF.
I worried that I was going to cause my skin not to look as good if I didn’t properly wash and rinse with water but this product is so amazing. I have not yet used it to see how well it removes makeup since I rarely wear any, but just for skincare and cleansing without a full wash with regular water, it is so amazing. Not going to sugarcoat it, this is me wanting a convenient routine when I travel but this is also me being lazy on nights when I’m very tired or mornings when I am in a hurry.
I consider this one of my few holy grail skincare products. I have picked up a smaller size for travel. I will keep this on hand always. I’m not even looking at moist wipes anymore. (This product is more gentle and leaves no residue as compared to a dermatologist recommended brand of face wipes). I’m most surprised that it cleans my face so thoroughly and that not rinsing does not cause any irritation or breakouts. Just wow. Totally worth the price. I was going to try other brands but this product has been so good there is no need to.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
A little goes a long way!
I'm almost 50 years old and have dry and super sensitive skin. I've struggled to find for years a gentle moisturizer, but effective on my fine lines as well. Omg this works! This gives my skin a beautiful non-oily glow, no negative reactions or breakouts, and leaves my skin so incredibly soft with elasticity. Within minutes it dries (or skin soaks it up) wonderfully, it's not sticky. I'm very pleased with this product, I will be purchasing it again, I hope this product remains the same, and stays on the market. I LOVE this product! I also have no need for my expensive moisturizer anymore, that was quite frankly not as nice as this. Keeps my skin moisturized and soft for 24 hours, fully tested this. I do use an eye cream, but only after I've used this over my entire face and neck after washing. Amazing product, no scent, awesome value for the money. Highly recommend!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 20, 2025
★★★★★ 5
Unique Texture, Amazing Results — Now One of My Favorites!
This serum definitely has a texture similar to snail mucin, so it took me a few tries to get used to — but I’m so glad I stuck with it because the results are incredible. Once it absorbs, my skin feels deeply hydrated, smoother, and so much more plump.
The rice and ceramide formula really strengthens the skin barrier, and I’ve noticed my complexion looking brighter and healthier overall. It layers beautifully under moisturizer and doesn’t leave any sticky residue once it settles in.
If you’re looking for a hydrating, brightening serum and don’t mind a unique texture, this one is absolutely worth it. It grew on me fast and is now a staple in my routine!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 4, 2025
★★★★★ 5
Great products
Helps with skin irritation does not leave a crazy residue. It is effective and it's an easy application! so the valuable for your money . They could definitely charge a little less, though. But all around great products by ANUA
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 5, 2026