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Description
Mouse TAGLN2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Urine: 4. Tissue homogenate: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a transgelin-2 (TAGLN2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of transgelin-2 (TAGLN2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Transgelin-2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transgelin-2 is a protein encoded by the TAGLN2 gene. The protein encoded by this gene is a homolog of transgelin, one of the earliest markers of differentiated smooth muscle. Diseases associated with TAGLN2 include Barrett's adenocarcinoma and maxillary sinus cancer. Pathways associated with this gene include responses to elevated platelet cytoplasmic Ca2+. Gene ontology annotations associated with this gene include binding to actin filaments. An important homolog of this gene is TAGLN3. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, urine, tissue homogenates and other biological fluids |
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4.7 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 3
THE PLATES ARE THIN
Size: 9 Inch, Style: Plates
THE PLATES ARE THIN AND MUST BE HELD FIRMLY TO AVOID SPILLAGE, ESPECIALLY IF UISED TO SERVE A WHOLE MEAL.
I AM USED TO STRONGER PAPER PLATES AND WILL NOT BE PURCHASING THIS TYPE AGAIN.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Absolutely Love It – Deep Hydration That Lasts!
Size: 8 Ounce (Pack of 1)
The Dove Body Cream Serum with Hyaluronic + Hydration has quickly become my go-to for tackling extra dry, flaky skin — and I absolutely love it!
💧 Deep, Lasting Hydration: From the first use, my skin felt instantly hydrated — and what’s even better? That hydration actually lasts throughout the day. No more reapplying!
🧴 Lightweight & Non-Sticky: It goes on smoothly, absorbs quickly, and doesn’t leave that greasy or sticky feeling — just soft, nourished skin.
🌿 Skin-Loving Ingredients: With pro-ceramides, niacinamide, and collagen peptides, it feels more like a high-end skincare product than a typical lotion. My skin looks visibly healthier, more even-toned, and has a soft glow.
✨ Bonus: It’s gentle enough for daily use and has a clean, fresh scent that’s not overpowering.
If you’re dealing with extra dry or flaky skin and want something that works and feels luxurious, this is it. Dove nailed it with this one — hydration, comfort, and glow in one bottle.
#DoveBodyCare #HydrationThatLasts #SoftHealthySkin #DrySkinSolution #SkincareMustHave #GlowUpWithDove
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Reviewed in the United States on July 24, 2025
★★★★★ 5
Soft Skin All Day Without That Greasy Feel
Size: 8 Ounce (Pack of 1), Size: 8 Ounce (Pack of 1)
My skin is dry and tends to get ashy, and after showering it can look worse, especially on my hands, elbows, knees, and heels.
I apply this while my skin is still slightly damp. It goes on creamy but absorbs quickly, so I don’t get that greasy or sticky feeling some lotions or body creams leave behind. I can get dressed right away without waiting.
It keeps my skin looking and feeling hydrated all day without needing to reapply.
The scent is light and clean, nothing strong or overpowering.
If your skin is severely dry or cracked, you may need something thicker. For my dryness, this does the job. It’s the one bottle I reach for every day.
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Reviewed in the United States on March 24, 2026
★★★★★ 5
Love this lotion
Size: 8 Ounce (Pack of 1)
Good value for the money, there isn't a strong fragrance (which I like) and Ive noticed the difference in my skin ,it looks and feels soft and hydrated. I will buy again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
★★★★★ 5
Disliked at first, ended up LOVING!
Size: 8 Ounce (Pack of 1)
UPDATE: I actually ended up loving this product once I started treating it as a first layer serum. I use a very small amount, let it soak in for a minute or two, and then layer a body lotion over it. Using it that way gives me the softest skim (even on rough dry areas like elbows) and it totally takes away any sticky/tacky feeling!
I don’t quite know how to feel about this product. It is hands down one of the most hydrating body lotions around and immediately after application skin does feel silky and smooth. It causes no irritation and has almost no scent. However the longer it is on the more tacky and sticky it gets- even dabbing extra off with a towel does not help. It is particularly bad on the hands, I literally could not stand it because it felt like I had partially dried glue all over my hands. Strangely the one place this lotion works on is my feet and it really helps with any dryness (I’m an avid walker and hiker so my feet get dry). I would not purchase again because I can’t use it anywhere else. I wish it stayed feeling like it does immediately after application because I bet it would help skin look and feel smoother with continued use, but unless the formula changes to something that isn’t tacky and sticky it is basically worthless. Unless you want to purchase as a foot cream!
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Reviewed in the United States on July 16, 2025