SKU: 60274393024

Human IL-9 ELISA Kit

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Description

Human IL-9 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. The specific anti human IL 9 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 9 in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human IL-9 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-9 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-9 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-9 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.06pg/ml

Detection Range: 7.81 -500 pg/ml

Recovery Range: 80-117%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 9 (IL-9)It is a glycosylated protein with a molecular weight of 14kda and contains 10 cysteine residues involved in disulfide bonding. It is a cytokine produced by T cells, especially cd4+ helper cells, and can regulate a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It acts by binding to the IL-9 receptor and can activate different signal transduction and activation (STAT) proteins, thus participating in a variety of biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies of asthma in mouse models have shown that this cytokine is a decisive factor in the pathogenesis of bronchial hyperresponsiveness. The presence of IL-9-mediated autocrine loops implies the presence of some malignancies such as Hodgkin's disease. IL-9 is expressed by Lee Stokes cells, Hodgkin lymphoma cells and some large aplastic lymphoma cells, but not by non Hodgkin lymphoma and peripheral T-cell lymphoma.

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SKU: 60274393024

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4.1 ★★★★★
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Verified Purchase
Lane
Grantham, US
★★★★★ 5
Perfect beginner set
Color: Lightgreen
Love this!!! The toothbrush heads are a little flimsy but overall really liked this for my 3 year old.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 23, 2026
C
Crunchy Life
Natrona Heights, US
★★★★★ 5
Super Cute, Fun, & Effective
Color: Lightgreen
This set of water flosser and electric toothbrush are just adorable! The shapes of them fit in the hands really comfortably, but also add to the cuteness as the shapes of the animals! Yes, this is meant for kids - so the toothbrush heads are small (for little kids), and the water flosser doesn't spray terribly hard. BUT they're still very effective. I especially like the water flosser. It can be taken into the bathtub/shower while bathing, so the spray doesn't end up going places we don't want water to go. It has a small tank of water, and I recommend using room temp water (rather than cold) so that it's comfortable on the gums and teeth. Also to note, the flosser doesn't work when it's sideways or upside-down - it needs to be held upright. This set is so cute, easy to use, easy to re-charge, and very comfortable. It's also very good quality - a great purchase. I love it!
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Reviewed in the United States on March 17, 2026
B
Bookmarked Opinions
Carnegie, US
★★★★★ 4
4 year old approved!
Color: Lightgreen, Color: Lightgreen
Love that this is a complete set, down to the wall block for the charger! My daughter is 4 and absolutely loves this set. I personally feel like the toothbrush head is a tad small, but it does do the job well. Its a soft brush head, with plenty of replacement heads. The toothbrush and the flosser both arrived fully charged, and so far we havent had to recharge it, but we're only a few days in. So, it seems to have a pretty good battery life so far. (I will update my review if anything changes on that aspect) The toothbrush and flosser both stand upright independently on the counter, without tipping over. The water flosser is hard fir my daughter to do by herself. She sprays water everywhere, as theres several buttons on it and she cant seem to remember in time which button turns it off. I help her with flossing and its been going fine. Whether youre on high or low the water pressure is not too hard for a kids mouth. In fact, I think the low setting does nothing but rinse the mouth and basically teach the habit of flossing. We use the high setting and it does and OK job. I am a little old school though, and feel like nothing will top a good ole dental floss flossing, but water flossing is a safer route to avoid cutting gums or getting floss stuck between close teeth. The sound level is minimal, a fraction of the sound level my adult water flosser makes. It doesnt shake/vibrate too badly in the hand and theyre bith comfortable to hold, and size appropriate for my (4yrs) daughters hands. It comes in a really nice box, packaged perfectly, making this a great gift for your little ones. The colors and theme makes brushing teeth exciting and fun again for your littles :)
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Reviewed in the United States on October 25, 2025
C
Cyclegreen
Whiting, US
★★★★★ 5
Great for kids
Color: Lightgreen
The HUACOCARE Kids Water Flosser and Electric Toothbrush Combo is an absolute game-changer for establishing a solid dental hygiene routine with children. The light green giraffe and monkey design is incredibly charming and immediately turned what used to be a bedtime struggle into a fun activity that my child actually looks forward to. Having both the sonic toothbrush and the water flosser in one compact unit is brilliant, as it saves counter space while ensuring that every part of their mouth gets a professional-level clean. The three gentle flossing modes and two sonic brushing modes are perfectly calibrated for younger, more sensitive gums, providing a thorough clean without any discomfort. One of the standout features of this set is how braces-friendly it is; the water flosser reaches those tricky spots around wires and brackets that a standard toothbrush simply can't touch. It is clear that a lot of thought went into the ergonomics and the "fun factor," making it a perfect gift that is both practical and engaging. Since we started using this combo, our dentist has noticed a significant improvement in plaque removal and overall gum health. If you are looking for an all-in-one solution to build healthy brushing habits and make dental care feel like less of a chore, this set is worth every penny.
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Reviewed in the United States on February 21, 2026
C
Verified Purchase
Coloratura Kiki
Dallas, US
★★★★★ 5
They Work!
Size: 60 Count (Pack of 1)
Thanks to my mouthrinse, I think, I have no issues with my teeth, gums, or breath, but cold air does inflame my sinuses, ETs, and ears. And I have to travel to cold places often. I hate that inflammation! As a classical singer, I really hate it! I have to keep my throat, sinuses, ETs and ears healthy. These probiotics have helped me to do just that. Previously, I tried prescription anti-inflammatories, and while they worked, anti-inflammatories can have undesirable side effects. I didn't experience any, but who knows? They could have been brewing. And I don't like chemical drugs. Much better to restore a healthy balance of bacteria in my upper respiratory system. These probiotics are so easy to take. I just let one dissolve in my mouth in the morning and one in the evening. I take them after breakfast, after brushing my teeth, and after brushing my teeth in the evening. It took a few weeks, but then I started seeing results. My sinuses were clearer, my ETs and ears would no longer close against the cold as they did previously. I no longer get the roaring in my right ear that I got when awakening due to sinus drainage. And I'm sure these probiotics are helping my teeth, gums, and breath even though I didn't really have any issues with them previously. If you decide to take these, and I highly recommend you do, be sure to let them dissolve or chew them slowly. To swallow them whole, which can be done easily, defeats their purpose since they are oral probiotics. And remember, these aren't probiotics for gut health. For that, you need a different probiotic mix, with different cultures. I do not refrigerate my probiotics, ever, and they remain potent. The taste is a light mint, and it's very agreeable. Not bad at all. Even if it were, the health benefits would definitely outweigh it. Bottom line: If you're experiencing any issues with your teeth, gums, breath, sinuses, throat, ETs, or ears, then these probiotics are for you! Try them! I think you'll be pleasantly surprised, and greatly pleased, just like I was!
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Reviewed in the United States on January 27, 2020

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