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Description
Human USP6NL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against USP6 N-Terminal Like Protein (USP6NL). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of USP6 N-Terminal Like Protein (USP6NL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human USP6 N-Terminal Like Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | USP6 amino-terminal-like protein (USP6NL), also known as UUSP6 N-terminal-like protein, is a protein encoded by the USP6NL gene. It acts as a GTPase activator for RAB5A and RAB43. It is involved in receptor trafficking. In complex with EPS8, it inhibits EGFR internalization. It participates in retrograde transport from the endocytic pathway to the Golgi apparatus. It participates in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network and is required for the structural integrity of the Golgi complex. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
A Breath of Fresh Air for Your Car
Size: 8.3" x 9.3" x 1.3"
This BOSCH 6037C HEPA Cabin Air Filter is a fantastic and simple upgrade for your vehicle. It delivers exactly what it promises: cleaner, fresher air inside your car.
The installation was incredibly straightforward. It was a perfect fit for my Acura RDX, and I was able to swap out the old filter in just a few minutes. You don't need any special tools, making this an easy and quick DIY project for anyone.
The difference in air quality is immediately noticeable. The HEPA filtration does a great job of trapping dust, pollen, and other allergens, leaving the cabin air feeling much cleaner. It also seems to reduce outside odors, which is a huge plus.
Overall, this is a high-quality product that provides excellent value. It's an essential and affordable way to improve the air quality in your car. I highly recommend it for any compatible Honda or Acura model.
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Reviewed in the United States on September 1, 2025
★★★★★ 5
High quality cabin filter
Size: 8.3" x 9.3" x 1.3"
I change out the cabin filters in my cars at every oil change. It help when running the A/C all summer. This is high quality cabin filter that does its job. Seeing what the dealer wants for literally 1 minute worth of work is silly.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
It works as expected
Size: 8.3" x 9.3" x 1.3"
Great. It does block air flow by a bit, but it is ok, if you don't need strong air blowing your face at maximum setting all the time.
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Reviewed in the United States on June 14, 2025
★★★★★ 5
Great cabin filter for 2019 Acura RDX
Size: 8.3" x 9.3" x 1.3"
When I have my car serviced at the Acura dealership, they routinely recommend that the cabin filter be replaced. As we all know, a dealership is going to charge an arm and a leg for a basic service. Therefore, I have been replacing the cabin air filter myself for the past few years. I like BOSCH products and when I found this filter on Amazon, I knew that was the one to purchase for my 2019 Acura RDX. The filter fits perfectly in its location in the glove box. It literally takes less than five minutes to swap out the old filter for the new. I will continue to use this product while I have the RDX.
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Reviewed in the United States on November 30, 2025
★★★★★ 5
Perfect fit, easy installation.
Size: 8.3" x 9.3" x 1.3"
This part fits perfectly. Directional flow is printed on the part however you need to confirm which way air flows in the actual air box. The easiest test is to run the a/c with the filter out, wet a finger, and stick in in the air box to see which side the air blows cold. Then just install this part in the air flow direction as printed on the part. I have not noticed a difference in air quality yet, however I imagine being a HEPA filter, this will do wonders keeping pollen out of the car. Installed this in a 2011 Honda CR-Z. Easy installation. Part looks high quality which is fitting for Bosch.
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Reviewed in the United States on May 26, 2026