SKU: 63285413927

Human IgG4 Fc ELISA Kit

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Description

Human IgG4 Fc ELISA KitProduct Specification Usage Operation steps 1. Dilution of standard product: This kit provides one original standard product. Users can dilute it in a small test tube according to the following chart. 20ng ml Standard No. 5 150l of primary standard was added to 150l of standard dilution 10ng ml Standard No. 4 150l of standard No. 5 was added to 150l of standard dilution 5ng ml Standard No. 3 150l of standard No. 4 was added to 150l of standard

Product Specification

Usage

Operation steps

1. Dilution of standard product: This kit provides one original standard product.
Users can dilute it in a small test tube according to the following chart.

20ng/ml

Standard No. 5

150μl of primary standard was added to 150μl of standard dilution

10ng/ml

Standard No. 4

150μl of standard No. 5 was added to 150μl of standard dilution

5ng/ml

Standard No.3

150μl of standard No. 4 was added to 150μl of standard dilution

2.5 ng/ml

Standard No.2

150μl of standard No. 3 was added to 150μl of standard dilution

1.25 ng/ml

Standard No. 1

150μl of standard No. 2 was added to 150μl of standard dilution

2. Sample addition: Set up blank wells (the blank control wells do not add samples and enzyme-labeled reagents, and the other steps are the same), standard wells, and sample wells to be tested respectively.
Accurately add 50μl of the standard on the enzyme-labeled coated plate, first add 40μl of sample diluent to the sample well to be tested, and then add 10μl of the sample to be tested (the final dilution of the sample is 5 times).
Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well.

3. Incubation: Incubate at 37 °C for 30 minutes after sealing with a plate sealing film.

4. Liquid preparation: 30 times concentrated washing solution is diluted 30 times with distilled water and used for later use

5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each hole with washing liquid, let it stand for 30 seconds and then discard it.
Repeat this 5 times and pat dry.

6. Enzyme addition: 50μl of enzyme-labeled reagent was added to each well, except for blank wells.

7. Incubation: The procedure is the same as 3.

8. Washing: The operation is the same as 5.

9. Color development: Add 50μl of color developer A50 μl to each well, then add 50μl of color developer B50 μl, gently shake and mix well, and develop color at 37 °C in the dark for 10 minutes.

10. Termination: Add 50μl of stop solution to each well to terminate the reaction (blue immediately turns yellow at this time).

11. Determination: Zero the blank hole, and measure the absorbance (OD value) of each hole sequentially at a wavelength of 450nm.
The determination should be performed within 15 minutes after the addition of the stop solution.

calculate

Taking the concentration of the standard substance as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find out the corresponding concentration from the standard curve according to the OD value of the sample;
Multiply by the dilution factor;
Or use the concentration of the standard substance and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply it by the dilution factor, which is the actual concentration of the sample.

Species Reactivity Human
Theory This kit uses double antibody sandwich method to determine the level of human immunoglobulin G4 Fc fragment (IgG4 Fc) in specimens. Coat the microplate with the purified antigen to make a solid phase antigen, add immunoglobulin G4 Fc fragment (IgG4 Fc) to the coated microwells in sequence, and then combine with HRP-labeled antigen to form an antigen-antigen-enzyme-labeled antigen complex, and then add substrate TMB to develop color after thorough washing. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and the immunoglobulin G4 Fc fragment (IgG4 Fc) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human immunoglobulin G4 Fc fragment (IgG4 Fc) in the sample was calculated by the standard curve.
Synonym Human immunoglobulin G4Fc fragment (IgG4 Fc) ELISA kit
Composition

Name

96 T

30 times concentrated wash

20ml × 1 bottle

Enzyme labeled reagent

6ml × 1 bottle

Enzyme-labeled coated plate

12 holes × 8 strips

Sample dilution

6ml × 1 bottle

Developer A liquid

6ml × 1 bottle

Developer B liquid

6ml × 1/bottle

Stop liquid

6ml × 1 bottle

Standard (40ng/ml)

0.5 ml × 1 vial

Standard dilution

1.5 ml × 1 vial

Sealing film

2 sheets



Specimen requirements

1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at-20 ℃, but repeated freezing and thawing should be avoided

2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

General Notes

1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 15-30 minutes before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag.

2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing.

3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples.

  • Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating.
  • The sealing film is limited to one-time use to avoid cross-contamination.

6. Please keep the substrate away from light.

7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader.

8. All samples, washing solutions and various wastes should be treated as infectious agents.

9. Components of different batch numbers of this reagent shall not be mixed.

Storage Temp. Store at 2-8 ℃, shelf life is 6 months.
Test Range 0.3ng/ml -22ng/ml
Shipping Notes
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Exchange/Return Notes
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SKU: 63285413927

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4.1 ★★★★★
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Madi lohr
Carnegie, US
★★★★★ 5
my new favorite book
Format: Kindle
Ok so I never write reviews but this book was so good I felt the need to write this. Firstly your introduced to Huntyr you see her closed off hard core badass than towards the end you see the most subtle change and growth it’s amazing and the enemies to friends to lovers was just perfect, AND THE TWIST AT THE END GOT ME GOOD! You see one spicy scene the whole book but it doesn’t even MATTER BECAUSE THE BOOK WAS THAT GOOD. I’ve read 85 books in 2023-2024 so far and I’m pround to say this is my all time favorite. I’m so excited to read more of Emily Blackwoods books, this was my first time reading one of hers and I’m glad I did because HOLY!! Well done Emily well done
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Reviewed in the United States on February 23, 2024
R
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Robin
Draper, US
★★★★★ 4
Fast paced romantasy you will not want to put down!
Format: Kindle
4.25 stars! I LOVED this book with similar vibes to Hush Hush, Fourth Wing, and The Serpent and the Wings of Night! It was fast paced with easy world building and will keep you turning the pages late into the night because you will not want to put it down! Huntyr is a fierce bad@ss FMC trained to kill vampyres her entire life. She is sent on a mission to go to the academy and earn her spot into The Golden City. Upon arrival, she is forced to room with the delicious fallen angel, Wolf, who is the only one who knows about her assassin identity. The romance, the plot twists, the secrets revealed, the battles, and the tantalizing training scenes had me hooked! And that ending…. I’m holding my breath in need to know hell! Read if you love: 🪽 Fae, Vampyres, Fallen Angels 🪽 Academy setting with magical trials 🪽 Forced proximity and slow burn 🪽 Rivals to lovers 🪽 Hidden identities and secrets 🪽 Tend your wounds “𝘖𝘧 𝘤𝘰𝘶𝘳𝘴𝘦 𝘐 𝘸𝘢𝘴 𝘸𝘢𝘵𝘤𝘩𝘪𝘯𝘨 𝘺𝘰𝘶. 𝘐 𝘤𝘰𝘶𝘭𝘥𝘯’𝘵 𝘭𝘰𝘰𝘬 𝘢𝘸𝘢𝘺 𝘧𝘰𝘳 𝘢 𝘮𝘰𝘮𝘦𝘯𝘵, 𝘦𝘷𝘦𝘯 𝘪𝘧 𝘐 𝘵𝘳𝘪𝘦𝘥.” “𝘐𝘧 𝘺𝘰𝘶 𝘸𝘢𝘯𝘵 𝘮𝘦 𝘰𝘯 𝘮𝘺 𝘬𝘯𝘦𝘦𝘴, 𝘏𝘶𝘯𝘵𝘳𝘦𝘴𝘴, 𝘢𝘭𝘭 𝘺𝘰𝘶 𝘩𝘢𝘷𝘦 𝘵𝘰 𝘥𝘰 𝘪𝘴 𝘢𝘴𝘬.” “𝘠𝘰𝘶 𝘥𝘰 𝘯𝘰𝘵 𝘬𝘯𝘰𝘸 𝘵𝘩𝘦 𝘷𝘪𝘰𝘭𝘦𝘯𝘤𝘦 𝘵𝘩𝘢𝘵 𝘳𝘶𝘯𝘴 𝘵𝘩𝘳𝘰𝘶𝘨𝘩 𝘮𝘺 𝘷𝘦𝘪𝘯𝘴, 𝘣𝘦𝘨𝘨𝘪𝘯𝘨 𝘮𝘦 𝘵𝘰 𝘰𝘣𝘭𝘪𝘵𝘦𝘳𝘢𝘵𝘦 𝘢𝘯𝘺𝘰𝘯𝘦 𝘸𝘩𝘰 𝘭𝘢𝘺𝘴 𝘢 𝘩𝘢𝘯𝘥 𝘰𝘯 𝘺𝘰𝘶.”
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 12, 2024
B
Verified Purchase
Bernadette Smith
Pawtucket, US
★★★★★ 5
Excellent Rivals to Lovers!!
Format: Kindle
The tension and banter between Huntyr and Wold was delectable. I absolutely love the fallen angel and all of his flaws. Huntyr is amazing too being a badass FMC with some major trauma. The world building was great and I enjoyed the training aspect of the story. The writing was immersive and was in the story the whole time. The ending had quite a twist that I hadn’t anticipated and made my jaw DROP. Excellent job! I also loved the narration. Laura is one of my fave narrators!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 15, 2025
E
Verified Purchase
❈ Elizabeth ❈ | Breakawayreads
Los Angeles, US
★★★★★ 5
Fallen Angels, fae, vampires, oh my!
Format: Kindle
Rating: 4.5 | Spice: 2 (but a good slow-burn) • Main Characters: Huntyr and Wolf • I couldn’t wait to read this book; there was so much hype about it! And there was no doubt why. I fell in love with the characters and the plot itself. This book is mainly plot driven more than friction driven but it’s easy to follow along with. The characters are fun, easily understood. The main setting is at an academy where both the main characters are going through trials and building strength for the final test, The Transcendent. There are fantastic side characters as well. I loved the camaraderie between Huntyr and her friends. But we don’t like Lanson. 😆 We do have some plot twists that come into play throughout the book. Secrets and betrayal to be seen. I did adore Wolf and Huntyr’s relationship. It was a classic slow burn trope. They didn’t hit it off fast, but in time their feelings grew. I loved their banter, so sexy. Wolf is your next book boyfriend; Huntyr is your next vampire assassin independent bad-a*s female. Themes include loyalty, trust, self-discovery, a true slow burn romance. Side note: book ends on a angsty cliffhanger! • Emily, thank you for writing this awesome novel and I cannot wait to devour Book 2, Blood So Brutal! 😍 • Happy reading, my lovelies! xo
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Reviewed in the United States on January 21, 2024
M
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MelsABookworm
Alexandria, US
★★★★★ 4
“My heart bows to you and you only, Huntress.”
Format: Kindle
3.5 🌟 This book popped up in my KU recommended reading suggestions and the synopsis sounded like what I was in the mood for. I'm so glad I took a chance on it. I went into this knowing absolutely nothing about it and ended up really liking it. I love when this happens. The main characters are likeable and I easily found myself rooting for them. There is a mystery element to each of their backstories that I enjoyed watching unfold and can't wait to get more of. Wolf, in particular, has me fixated. Love him. I found this to be an entertaining, addictive read with a plot that moves along at a good pace. It reads so easily I found myself very reluctant to put it down. Lots of twists and turns and the angst is there. A good set up for the next book to come, for sure. My issues with this book....the dialogue feels a bit juvenile at times and there is a repetitive over use of a particular word phrasing that I found myself giving the ole eye-roll to. There are, without a doubt, some pretty cliche moments that gave me a bit of the cringe. I think this could've certainly 100% benefited from more depth regarding the world building. Perhaps the world building was sacrificed to keep the pacing quick? Just a guess. Also, the lack of consistency of character for the FMC was really evident and so she feels quite illogical at times. Overall, this was a fun and enjoyable read that hit the spot well enough for me. That ending certainly has me impatiently pining for book 2!
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Reviewed in the United States on June 18, 2024

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