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Description
Rat CASP12 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a caspase 12 (CASP12) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of caspase 12 (CASP12) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Caspase 12 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Caspase 12 (CASP12) is a protein encoded by the CASP12 gene. This protein belongs to a family of enzymes known as caspases, which cleave their substrates at the C-terminal aspartate residue. It is closely related to caspase 1 and other members of the caspase family, known as inflammatory caspases, which process and activate inflammatory cytokines such as interleukin 1 and interleukin 18. This gene is polymorphic, producing either the full-length caspase protein (Csp12L) or an inactive truncated form (Csp12S). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.3 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Great collection idea!
Color: Kansas, Size: 200 pieces
Super quick shipping - arrived as described! big hit
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 29, 2025
★★★★★ 5
Perfect Pocket Sized Puzzle
Color: Fifa Football, Size: 26PCS, Color: Fifa Football, Size: 26PCS
Although this puzzle is much smaller than I initially expected, it is exceptionally well-made and serves as a fantastic collectible for any soccer fan. This FIFA World Cup 2026 Mini Wooden Puzzle is an officially licensed piece of merchandise celebrating the upcoming tournament hosted by the USA, Canada, and Mexico.
Measuring just 4 x 4 inches, this 26-piece puzzle is designed in the shape of a soccer ball and features the iconic World Cup trophy. Despite its small piece count, it offers a fun and engaging challenge for both children and adults. The high-quality wood construction makes it durable and gives it a premium feel compared to standard cardboard puzzles.
Key Highlights:
Its compact size makes it incredibly portable. It fits easily into a bag or backpack, making it perfect for travel, game nights, or World Cup watch parties.
This is a unique and affordable gift for soccer enthusiasts. It’s an ideal choice for birthdays, Father’s Day, or even as a high-quality party favor for fans of all ages.
As official 2026 World Cup gear, it is a great keepsake to commemorate the tournament.
If you are looking for a small but meaningful gift for a football fan, or a quick activity to improve focus and patience, this mini wooden puzzle is a great pick.
As an Amazon Vine member, I personally select products to review. I tend to choose items that align with my interests and needs, which often leads to positive 4- and 5-star ratings. However, if a product does not meet my expectations, or it is not a good value, I will always provide an honest and transparent assessment of its performance. EVERYTHING starts at a 5, and goes from there. I thoroughly read the descriptions and if I don't like the description, I don't order the item. If the product shows up as advertised they get a 5. If there are unadvertised flaws, or if the information or photos are misleading, it is noted and stars removed accordingly.
All opinions expressed in my reviews are my own, and on occasion, I may include feedback from my family or friends to offer a more comprehensive perspective.
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Reviewed in the United States on April 18, 2026
★★★★★ 2
Cheap broken box and no bag, allow pieces to fall out and are now missing….
Color: Fifa Mascot 2, Size: 26PCS, Color: Fifa Mascot 2, Size: 26PCS
Tiny little puzzle with no picture and missing pieces….
What you should receive is a very small box, a little larger than a lighter or the size of a key fob. The box is very light weight and flimsy. Mine arrived crushed on the bottom corner and ripped. This is similar to a candy box. Same type and weight. The pieces are not in a bag at all so when my box arrived in the condition it was in, and nothing sealed, of course pieces are missing. There are supposed to be to be 26 and I don’t have but half. They all fell out of the box like candy falls out of a broken box.
The puzzle itself is done by laser and the pieces I do have fit together well but, the color is off. I believe they use a stain and the ink has spread into the other pieces next to them. The color is deep and intense and most is accurately done but some pieces have multiple colors that are not in the right place. Some pieces I can’t tell which side is the front and which side is the back. The pieces are also so small, with so much information crammed on, I can’t make it out as I work on it or once it’s completed the best I can with only part of the pieces. I am disappointed with the whole thing and can only say the laser cutting was done well. Other than that, I wouldn’t order again, especially for a souvenir.
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Reviewed in the United States on April 18, 2026
★★★★★ 3
Durable Wooden Puzzle, but the Visuals are Disappointing
Color: Fifa Mascot 1, Size: 26PCS, Color: Fifa Mascot 1, Size: 26PCS
I picked this up to get ready for the 2026 World Cup hype. While it’s a cool idea for a collectible, the final result is a bit of a letdown visually.
The Material
The best thing about this puzzle is that it’s made of wooden pieces rather than the typical thin cardboard you find in cheap puzzles. This gives it a nice weight and makes it feel much more durable. The pieces fit together well and it feels like it will last a long time.
The Problem: Distracting Black Outlines
The main reason for the 3-star rating is the printing and cut quality. As you can see in my photos, the black outlines around every single piece are way too noticeable.
Instead of the pieces blending together to create a smooth, clean image of the World Cup graphics, the whole thing looks "choppy" and fragmented.
Up close, the black seams really take away from the design, making the overall picture look less premium than it does in the stock photos.
Final Verdict
If you just want a quick, sturdy wooden activity for a soccer fan, this is fine. But if you’re looking for a high-quality display piece to show off your 2026 excitement, the heavy black lines might bother you. It’s a decent "mid-tier" collectible, but it could have been much better with a more seamless print.
Pros:
Solid wooden construction (much better than cardboard).
Officially branded 2026 World Cup item.
Easy to handle pieces.
Cons:
Heavy black outlines break up the image.
Doesn't look very "clean" once fully assembled.
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Reviewed in the United States on April 22, 2026
★★★★★ 5
The Ultimate Puzzle for Soccer Fans – Beautiful, Challenging & Totally Frame-Worthy ⚽🧩
Color: Wa 26 Ball-200, Size: 200Pcs
As someone who absolutely loves puzzles and is a huge World Cup fan, this one felt like it was made just for me. It combines two of my favorite things—a fun, satisfying puzzle experience and meaningful, display-worthy art—and honestly, it exceeded my expectations.
The design is stunning. The “We Are 26” FIFA World Cup theme gives it that special, collectible feel, especially knowing the 2026 tournament will be hosted across the U.S., Canada, and Mexico. It feels like a piece of history in the making, and that makes completing it even more exciting.
The 200-piece count is perfect for a relaxing but engaging session. It’s not overwhelming, but still gives you that satisfying challenge. I loved how immersive it felt—great for unwinding without staring at a screen, and it really keeps your mind focused and engaged.
What truly sets this puzzle apart is the quality of the wooden pieces. They’re thick (5mm!) and durable, with a really nice weight to them. They fit together beautifully and feel sturdy enough that once completed, the puzzle holds its shape—which is ideal if you plan to display it.
And yes… this is absolutely frame-worthy. The finished 11.8 x 11.8 inch size is perfect for wall décor, and the design looks so clean and polished once complete. I love turning my puzzles into art, and this one looks amazing in a home office or living space—especially for anyone who loves soccer.
Why I love it:
* Official FIFA World Cup 2026 design with real collectible value
* Perfect balance of challenge and relaxation
* Thick, high-quality wooden pieces that feel durable and premium
* Beautiful finished look—ideal for framing or gifting
* Great for puzzle lovers and soccer fans alike
Perfect for gifting: I’ve already decided this will be one of my go-to gifts for friends and family who love soccer. It’s thoughtful, unique, and something they can enjoy and keep.
Final thoughts: If you love puzzles that you can complete, admire, and proudly display, this one is a standout. And if you’re also a World Cup fan? It’s truly the best of both worlds. ⚽✨
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Reviewed in the United States on April 25, 2026