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Description
Human IL-22 Kit (HICA)Product Specification Stability & Storage Store at 2~8C protected from light for 12 months; after reconstitution, the standard can be aliquoted and stored at 20C, avoiding repeated freeze thaw cycles. Background Principle of the assay: This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) based on the double antibody sandwich principle for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
Product Specification
| Stability & Storage | Store at 2~8°C protected from light for 12 months; after reconstitution, the standard can be aliquoted and stored at -20°C, avoiding repeated freeze-thaw cycles. |
Background
Principle of the assay:
This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) based on the double-antibody sandwich principle for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
The detection system consists of two types of microspheres: acceptor microspheres conjugated with antibody 1 specific to the target protein, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two types of microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated by light excitation can transfer to the acceptor microspheres, triggering chemiluminescence. Conversely, if the target protein is absent, the microspheres remain too far apart, resulting in no signal.
By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method offers advantages such as simplicity, rapid reaction, and high sensitivity.
Components
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Name |
Specification |
Component Specification |
Detection Reagent R1A |
500T |
2mL/vial×1 |
2000T |
8ml/vial×1 |
|
10000T |
40ml/vial×1 |
|
Detection Reagent R1F |
500T |
2mL/vial×1 |
2000T |
8ml/vial×1 |
|
10000T |
40ml/vial×1 |
|
Detection Reagent R2 |
500T |
2mL/vial×1 |
2000T |
8ml/vial×1 |
|
10000T |
40ml/vial×1 |
|
Detection Reagent R3 |
500T |
5mL/vial×1 |
2000T极span> |
20ml/vial×1 |
|
10000T |
100ml/vial×1 |
|
Standard |
500T |
0.5μg lyophilized product×1 |
2000T |
0.5μg lyophilized product×2 |
|
10000T |
0.5μg lyophilized product×5 |
|
Standard Buffer |
500T |
6mL/vial×1 |
2000T |
12ml/vial×1 |
|
10000T极span> |
30ml/vial×1 |
Note: The recommended plate is a microplate (384 or 96 wells, white, shallow well)
```Guidelines
Reagent R3 should be protected from light during use. Sampling and incubation are recommended to be performed under green light (<100 LUX).
Recalibration is required for each test. Each concentration point of the standard should be tested in at least duplicate wells, and the calculation should be performed using a four-parameter (weight 1/Y²) fitting method.
Temperature and time should be carefully controlled during incubation. Microplates should be covered with film, and a microplate reader with ALPHA functionality is recommended.
The dilution matrix of the calibrator should be consistent with the sample matrix. The reconstituted solution should be used within 2 hours.
Components from different reagent kit batches must not be mixed.
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