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Description
Rat vHL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 x g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store the supernatant at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000 x g for 20 minutes. Remove the supernatant for testing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and allow it to equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a von Hippel Lindau Tumor Suppressor (vHL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of von Hippel Lindau Tumor Suppressor (vHL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | ELISA Kit for Von Hippel Lindau Tumor Suppressor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 5
Almost a year after purchase and this printer has not let me down!
Color: Black, Style: ET-2800-B, Pattern Name: Printer
I wanted to wait to write a review on this printer to see how it held up over time. Happy to report, after almost a year, it is still working perfectly! I was very nervous about purchasing a printer since it was my first time having to shop for one and almost every printer I looked at had something negative to say about the product. After extensive research, I decided to take a leap with the Epson EcoTank 2800.
I was looking for a printer to use for recreational use. Something with decent print quality and longevity as well as something on a reasonable budget. I normally print full pages of color and have been using it regularly for around a year and I have about half of the ink that came with the printer left. Safe to say, the ink lasts a while. The quality may not be the top of the line quality you can get with a more expensive printer, but for the price and just everyday use, the quality is satisfactory. It was easy to set up and walked you through the instructions to connect to devices and configure it. The screen is a little small but if you can overlook that, it really is a nice printer. I have used regular printer paper, card stock, and sticker paper with this printer and I try to load only a few sheets at a time to avoid a jam but I haven't had any issues with it so far.
Overall, I am extremely happy with my purchase. It can be extremely difficult to find a decent printer and some reviews can be misleading or leave you with questions on if the product is for you. If you are looking for something for printing everyday with decent quality and ink consumption that won't cost you a fortune, this printer is definitely worth taking a chance on!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2026
★★★★★ 4
Printing is great, wifi is a problem (I figured out how to make it work!)
Color: White, Style: ET-2800-W, Pattern Name: Printer
I was on the fence about buying this printer because most of the 1 star reviews all said the same thing... "The Wifi would not work"
So I followed the instructions, Turned on the print, downloaded the IOS app, connected to the printer via Bluetooth, installed the ink, initialized the printheads. While that was going on, the app asked me to setup the wifi. a List of SSID's came up and I selected the one I wanted. I put in the password and it connected and the icon on the printer turned blue. I have a Unifi setup and I could see that the printer connected to access point #3 @ 2.4ghz and got an ip address. I locked the IP address to the printer.
(For my instructions to work for you, you need to make a reservation or lock the ipaddress that your DHCP server provided to the printer. ) If you don't do this, you will get a different address every time you turn the printer on and the computer and app will not be able to find the printer.
When the initialization was complete, the app on my phone said it could not connect to the printer. I decided to try to connect to a computer and come back to the phone app. I downloaded the software package from Epson and ran it. When it went to connect to the printer it could not find it and brings up a list of troubleshooting steps that are not likely to do anything.
There are videos about this problem and they are all useless. I am an IT professional with 26 years of experience. I am not going to unplug the printer and wait five minutes, I am not going to re-boot my router, I have a Unifi network environment with over 150 devices connected... (I have great wifi coverage, I am not moving my printer anywhere) My network works just fine, this printer is the issue!
So at this point, I know that the printer IS connected to my wifi and it got an IP address. This is not a wifi problem as everyone seems to think it is. It is an issue with the Epson software.
I decided to try to install it via the windows printer installer. I selected install via TCP/IP and put the ipaddress that my Unifi system provided to the printer. Windows found the printer and since the Epson app had already installed the drivers... just asked me what drivers I wanted to use. The ET-2800 showed up on the right hand side of the screen. I selected it, it installed and I was able to try a test print. It worked fine. I can print from my computer to the printer via the network.
Next was the phone app. The phone app has the option to find the printer via the IP address. I put the IP address in and it found the printer. The phone app works now too.
So, It took me some time to get this working, it should not have taken any time at all if the software worked correctly. The printer is really nice, it is a shame that the software is so bad when it comes to connecting to the printer.
So if you decide to buy one of these printers, save yourself the headache and just install using the ip address, it is quick and easy that way and it just works. I would have just purchased an HP printer, but I did not want to deal with all the issues of their subscription ink service. I feel like the cost of ink makes this printer worth the hassle of getting it to work.
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Reviewed in the United States on December 2, 2024
★★★★★ 5
Premium Print Quality Meets Economic Design
Color: White, Style: ET-2803, Pattern Name: Printer
I am very impressed with the manufacturer quality of this printer. It feels durable and well-engineered. The print quality is equally excellent; colors are vibrant and text is sharp, easily meeting the standards I need for both documents and photos.
Moving away from cartridges is a huge win. The ink lasts incredibly long, and the refill process is clean and simple.
The only thing I miss is a USB port for printing directly from external storage. My old HP printer had this feature, and it was extremely useful for printing files quickly without a computer. I wish this Epson model included that same convenience.
Final Verdict
A high-quality, reliable printer that produces great results. If you don't mind the lack of a direct USB drive slot, it’s an outstanding choice for home use.
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Reviewed in the United States on April 19, 2026
★★★★★ 5
Handles Photo-Quality Prints, Versatile Connectivity
Color: Black, Style: ET-2800-B, Pattern Name: Printer, Color: Black, Style: ET-2800-B, Pattern Name: Printer
I've had this printer for a whole year now. I was on a severe budget, looking for a photo-quality printer that didn't require Wifi in this digital age- because I quite frankly can't afford my own Wifi connection. My rink-a-dink laptop had to be set up with the software at my library, and an extra cord purchased.
All that said, I mostly print from my phone, because the software app is a bit easier to ensure that I get the highest quality setting. Laptop loves to be a snot about it. The blutooth feature, or "wifi direct", has a way about it, and as soon as you figure out what it wants, it gives minimal problems.
It functions exactly as if a printer had wifi on it, believe it or not.
What sold me on this model was the ability to do all of that, and adjust for different situations, seeing as other brands DEMAND constant wifi connectivity (lookin' at BROTHER, maybe Canon, too), and the long lasting ink tanks- I've printed hundreds of pages, and being a gothic/horror artist, at least a good part of the time, black is the only color even half gone.
What horrified me the most about printer-shoping dropping a lot of money (hundred plus is a lot over here, man) and not getting the prints to look like my work. Well, fear not. The attached pictures are why I made this review, to hopefully ease the anxiety of others. I make digital paintings, and use high DPI for high quality, and also the cutest micro-stickers you've ever seen. Prints come out clear, even if they can sit in the tip of my finger.
Yes, there are probably better printers. Maybe they're faster, and have even better connectivity, and have more bells and whistles. I specifically did not want to pay for those things, so, I'm very happy there's options.
It's not perfect, no, because every now and then, I get what I call a "special edition", where my printer has decided to multi-color barf out the last third of the page. One time it randomly printed in black and white. Well, that's no big deal, my stuff tends to look great in monochrome. Every now and then I get a low quality print; things just look a bit fuzzy. I'm not happy about that, but, you clean the heads, and print something simple and toony, and it has never done it twice in a row. Alignment has been off only one time, and I put the printer away after every session.
It occurs to me that people pay good amounts of money for printers that do that sort of thing anyway, so... Printers gonna printer, no matter where you try to hide.
Overall, I'm happy and relieved. I hope it lasts me a good long time. (Streetratt on Kofi, by the way.🖤 )
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 24, 2026
★★★★★ 5
Great functional printer
Color: Black, Style: ET-2800-B, Pattern Name: Printer
This is a great basic printer for my office. We have a higher model in our main floor office but it is older. The setup is fast and easy and it prints well. I set it up right from my phone. The picture quality is great and it prints quickly. It is so convenient and was the right price for what I need.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
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