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Description
Human PPAR-α ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against peroxisome proliferator-activated receptor alpha (PPAR-α). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of peroxidase (HRP) and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of peroxisome proliferator-activated receptor alpha (PPAR-α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Peroxisome Proliferator Activated Receptor Alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Peroxisome proliferator-activated receptor α (PPAR-α), also known as NR1C1 (nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein encoded by the PPARA gene. Along with peroxisome proliferator-activated receptor δ and peroxisome proliferator-activated receptor γ, it belongs to the peroxisome proliferator-activated receptor subfamily. It was the first member of the PPAR family cloned by Stephen Green in 1990 and was identified as a nuclear receptor for a distinct class of rodent hepatocarcinogens that promotes peroxisome proliferation. It is primarily activated by ligand binding. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.0 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
Handsome Throw
Very pleased with the throw. Appearance is very realistic and beautiful.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
★★★★★ 5
Soft, Stylish Leopard Blanket That’s Lightweight, Cozy, and Big Enough to Share
I have been using this leopard print blanket on both the bed and the couch, and it hits a really nice balance between style, size, and comfort. I went with the larger twin style size instead of a small throw, and that was the right call; it is big enough to fully cover me when I am lying on the couch and works much better for two people sitting side by side than a standard throw that always feels a bit cramped. It also looks great draped across the end of a bed or over the back of a sofa, so it doubles as decor.
The leopard pattern is genuinely attractive in person. Up close you can see that it is a printed, short shag/flannel style rather than true faux fur, but the brown leopard version still reads more realistic and intentional than a lot of loud, pink “leopard” prints. The colors are vibrant and have a nice pop without being tacky, so it fits easily into different rooms and color schemes as a fun accent. From a distance, it gives a soft, faux fur vibe that looks more expensive than it is.
Comfort wise, it is very soft and pleasant to snuggle with. One side feels just slightly softer than the other, but both are smooth and cozy against the skin. It is a single layer blanket with rolled and stitched edges, so it is not a super thick winter comforter, but that actually makes it more versatile. It provides enough warmth to feel comforting without being overly hot, which makes it a nice year round piece: cozy in cooler weather with an extra layer, and just right on its own in milder seasons.
The blanket is also lightweight, which makes it easy to move around the house or pack for travel, while still feeling substantial enough that it does not come across as flimsy. It has held up well so far, and the overall quality feels good for the price, especially given the larger size. If you love animal prints and want something that works as both decor and a genuinely comfy blanket to curl up with, this one is easy to recommend.
Pros:
Large twin style size is big enough to fully cover an adult or share on the couch
Soft, plush flannel style fabric that feels cozy against the skin
Lightweight but still warm enough to be comfortable year round
Brown leopard print looks stylish and more realistic than many novelty patterns
Colors are vibrant and work well with a variety of room styles and palettes
Edges are neatly rolled and stitched, giving it a clean finished look
Cons:
Single layer construction means it is not as heavy or insulating as a thick winter blanket
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Reviewed in the United States on April 22, 2026
★★★★★ 4
Cute
Beautiful but a little too light for me like a little more heaviness
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Super Cozy & Stylish – My New Favorite Throw Blanket
I recently got this brown leopard print throw blanket, and it quickly became one of my favorite cozy essentials at home. The first thing that stood out to me was the trendy animal print design—it adds a bold, stylish touch to my living room and makes my couch look instantly more put together.
The material is incredibly soft and plush. It has that fluffy flannel feel that’s perfect for snuggling, whether I’m watching TV, reading, or just relaxing. It’s warm without being too heavy, which makes it great for using all year round.
I also really like the size (60" x 80")—it’s big enough to fully wrap around me or share when sitting on the couch. It works just as well on the bed as it does as a decorative throw.
Another plus is how lightweight and easy to carry it is. I’ve even taken it along for road trips, and it’s been perfect for staying comfortable on the go.
If I had to point out one small downside, it’s that lighter-colored fabrics can show wear over time if not cared for properly, so I make sure to wash it gently to keep it looking fresh.
Overall, I’m really happy with this blanket. It’s the perfect mix of comfort and style, and it adds a cozy, fashionable vibe to any space. Definitely a great pick if you love soft textures and bold décor!
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Reviewed in the United States on April 30, 2026
★★★★★ 5
Soft, Warm, and Holds Up Well After Washing
We grabbed this primarily for couch lounging and it has been a regular rotation blanket since it arrived. The softness is legitimate, not the kind that feels good out of the packaging and then stiffens up after a wash or two. It has gone through the machine multiple times and both the texture and the leopard print have held up without fading or pilling. The 50 by 60 inch size is a comfortable fit for one person stretched out on the couch.
It is warm without being heavy, which makes it practical across seasons rather than just winter. The print looks sharp and adds some personality to a living room without being over the top. For a throw blanket at this price, the quality is better than expected and it has earned a permanent spot on our couch. If you are looking for something soft, visually interesting, and durable enough to actually use regularly, this one delivers.
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Reviewed in the United States on May 12, 2026
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