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Description
MT ELISA KitProduct Specification protein MT Usage Self prepared test equipment required for the experiment: 1 , plate reader ( 450nm 2 , high precision sampler and gun head: 0. 5 10uL 5 50uL 20 200uL 200 1000uL 3 37 Incubator 4 Distilled water or deionized water, Sample handling and requirements: Serum: Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4 Overnight, then 1000g Centrifugation 20 Minutes, take the
Product Specification
| protein | MT | |||||||||||||||||||||||||||||||||
| Usage |
Self-prepared test equipment required for the experiment: 1 , plate reader ( 450nm ） 2 , high-precision sampler and gun head: 0.5-10uL 、 5-50uL 、 20-200uL 、 200-1000uL 3 、 37℃ Incubator 4 Distilled water or deionized water, Sample handling and requirements: Serum: Whole blood samples collected in serum separation tubes were placed at room temperature 2 Hour or 4℃ Overnight, then 1000×g Centrifugation 20 Minutes, take the supernatant, or place the supernatant in -20℃ Or -80℃ Store, but repeated freezing and thawing should be avoided. Plasma: use EDTA Or heparin as an anticoagulant to collect specimens, and collect the specimens after collection 30 Within minutes 2-8℃1000×g Centrifugation 15 Minutes, take the supernatant to detect, or place the supernatant in -20℃ Or -80℃℃ Store, but repeated freezing and thawing should be avoided. Other biological fluids: 1000xg Centrifugation 20 Minutes, take the supernatant to detect. Preparations before testing: 1 , please advance 10 Minutes remove the kit from the refrigerator and equilibrate to room temperature. 2 , Standard gradient working solution preparation : join 1mL Universal Diluent into Lyophilized Standard , let stand 15 Minutes until it is completely dissolved and then gently mix (the concentration is 500pg/mL ） And then according to the following concentrations: 500pg/mL 、 250pg/mL 、 125pg/mL 、 62.5pg/mL 、 31.25pg/mL 、 15.625pg/mL 、 7.8125pg/mL 、 0pg/mL The dilution was performed. Double dilution method: Take 7 branch EP Tubes, each tube is added 500uL Universal diluent ,500pg/mL Pipette from the standard working solution 500uL To the first EP Mix evenly in a tube 250pg/mL Standard Working Solution , according to this step, absorb and mix evenly in turn. The last tube is directly used as a blank hole, There is no need to draw liquid from the penultimate tube, as shown in the figure below. 3 、 Biotin- Preparation of antibody working solution : Before use 15 Minutes will concentrate Biotin- Antibody to 1000×g Centrifugation 1 Minutes, with a universal diluent 100× concentrate Biotin- The antibody is diluted into 1× Working concentration (ex: 10uL Concentrate +990uL Universal Diluent) , used on the same day. 4 、 Preparation of enzyme conjugate working solution : Before use 15 Minutes will 100x Concentrated enzyme conjugate in 1000×g Centrifugation 1 Minutes, with a universal diluent 100× concentrate HRP The enzyme conjugate is diluted into 1× Working concentration (ex: 10uL Concentrate +990uL Universal Diluent), for same day use. 5 、 1× Wash liquid preparation: Take 10mL 20× Wash liquid to 190mL In distilled water (the concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. It can be left at room temperature and prepared after the crystals are completely dissolved). Operation steps: 1 Equilibration from room temperature 10 After minutes, remove the required slats from the aluminum foil bag, and seal the remaining slats with ziplock bag and put them back 4℃ 。 2 , Adding samples: respectively add samples or different concentration standards according to 50ul Each well is added to the corresponding well, and the blank well is added 50uL Universal dilution followed by each well 50uL Biotin- Antibody working fluid. After covering the sealing film 37℃ Incubation 1 Hours. (Recommendation: minimum dilution of sample to be tested with universal diluent 1 Then add enzyme labeled in-plate test, so as to reduce the error of matrix effect on test results , influence, and finally calculate the sample concentration by multiplying it by the corresponding dilution factor. It is recommended to set up double wells for all samples and standards to be tested during testing). 3 Plate washing: discard the liquid and add to each well 300uL 1x Wash liquid, stand 1 Minutes, throw off the washing liquid, pat dry on absorbent paper, and repeat washing the plate 3 Times (the plate can also be washed with a plate washing machine). 4 Adding enzyme conjugate working solution Enzyme conjugate working solution is added to each well 100uL , after covering the sealing film 37℃ Incubation 30 Minutes. 5 、 Washing plate : Discard liquid according to step 3 Washing Method , wash plate 5 Times. 6 Substrate addition: substrate is added per well ( TMB ） 90uL Covered with a sealing film, 37℃ Incubation protected from light 15 Minutes. 7 Add stop solution: take out the enzyme label plate and directly add stop solution to each well 50uL , immediately in 450nm Wavelength measurement of each well OD Value. Calculation of experimental results: Result judgment: 1 , calculate the average of the standard and sample replica well OD Value and subtract the blank hole's OD Values as correction values. Taking concentration as the abscissa, OD Value is ordinate , draw the standard curve of the four-parameter logic function on the double logarithmic coordinate paper. 2 If the sample OD If the value is higher than the upper limit of the standard curve, the test should be retested after appropriate dilution and multiplied by the corresponding dilution factor when calculating the sample concentration.
Kit Performance: 1 Repeatability: the coefficient of variation in the plate is less than 10% , the interplate coefficient of variation is less than 10% 。 2 Recovery rate: adding to selected healthy serum and plasma 3 Of different concentration levels MT , calculate the recovery.
3 , linear dilution: respectively in the selected 4 Healthy serum and plasma are added with high concentration MT , linearity was assessed by dilution within the standard curve kinetic range.
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| Theory | This kit uses competitive enzyme-linked immunosorbent assay (ELISA). The sample, standard, Biotin-labeled antibody, and HRP enzyme conjugate were sequentially added to the microwells pre-coated with the universal species Melatonin (MT) antigen, incubated and washed in the middle, and the substrate TMB was used to develop color. The TMB was converted to blue under the catalysis of peroxidase (HRP), and to the final yellow under the action of acid. The depth of color was positively correlated with the universal species Melatonin (MT) in the samples. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated. | |||||||||||||||||||||||||||||||||
| Source | General purpose | |||||||||||||||||||||||||||||||||
| Synonym | Melatonin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | melatonin, or melatonin or melatonin, is chemically known as N-acetyl-5-methoxytryptamine. In universal species, serotonin (also known as serotonin) is converted to N-acetyl-5-hydroxytryptamine by reaction with acetyl-CoA catalyzed by N-acetyltransferase, which is then methylated to melatonin by S-adenosylmethionine catalyzed by acetyl-serotonin O-methyltransferase. The side effects of melatonin supplements are minimal when given in small doses, short courses. They may include somnolence (drowsiness), headache, nausea, diarrhea, abnormal dreams, irritability, nervousness, restlessness, insomnia, anxiety, migraine, drowsiness, psychomotor hyperactivity, dizziness, high blood pressure, abdominal pain, heartburn, mouth ulcers, dry mouth, hyperbilirubinemia. Dermatitis, night sweats, itching, rash, dry skin, pain in extremities, menopausal symptoms, chest pain, glycosuria (sugar in urine), proteinuria (protein in urine), abnormal liver function tests, weight gain, fatigue, mood swings, aggression and a feeling of hangover. It is not recommended for use during pregnancy or breastfeeding or in generic species with liver disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Carry out incubation in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Store reagents in refrigeration immediately after use. 2. Incorrect plate washing may lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the wells to dry out during incubation. 3. Eliminate the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value. 4. The substrate color development solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used. 5. Avoid cross-contamination of reagents and specimens to avoid wrong results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reaction reagents in the kit. 8. Expired products cannot be used, and components with different item numbers and batch numbers cannot be mixed. 9. Recombinant proteins from sources other than the kit may not match the antibodies in this kit and are not recognized. 10. If the disease may be spread, all samples should be managed well, and the samples and testing devices should be handled according to the prescribed procedures. |
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| Storage Temp. | Unopened kit, stored at 4 °C, shelf life 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.8-500pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma and other biological fluids |
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4.0 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Price / Quality - Office Desk - Perfect
Size: H Desk Unfinished 3/4-in and 1/2-in
Purchased two sets to create a custom L shaped desk for the office. Placed a pine stained top. Perfect to handle a monitor, multiple laptops, normal office equipment. Extra benefit is the cross piece at the bottom as a foot rest. Very sturdy while sitting on carpet. Compared to normal pipe material purchased in large box store -- could not have asked for a better product for the price. Looking at other products for additional projects for around the house for the product and vendor. - Highly Recommend.
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Reviewed in the United States on April 27, 2021
★★★★★ 4
Fantastic style, good stability, customizable finish, and great customer service
Size: M Desk Unfinished 3/4-in and 1/2-in
Put this together in about April 2020 right about the crazy lockdown/quarantine/work-from-home timeframe. I knew that I wanted a simple hardwood desk with no drawers or stuff below the top. I have an ergonomic keyboard tray that I have attached to all my work desks for many years now, and it was too long for my old home desk, which was also made of MDF and was falling apart. Boo.
Enter this kit plus a butcher block top from the big box store. This kit went together easily. I had lost the instructions but a quick email to the JS Supplies company, and they hooked me up. By the time they sent it, I had actually figured out the part that was puzzling, which was what the all-thread pieces were for (the couplers between the elbows and the flanges on the top side).
I degreased all the parts with a simple Simple Green-type solution diluted in a 5 gallon bucket. I then sprayed the pieces with a satin nitrocellulose laqcuer (Minwax brand that I got from the home improvement big box). Worked great. I thought about doing a sealed rust finish using a science project volcano--vinegar and baking soda for a quick layer of rust on the outside--but I decided against it, as I wasn't sure of how stable the final product would be, even after a clear spray finish. The satin nitro finish looks great without even looking like a finish. I also coated the pieces with paste wax after the finish dried, just for good measure.
The one thing that is a bit disappointing is the stability... WITH MY TOP, mind you.I should be clear that the top that I'm using is a 1-3/4" thick monster piece of birch butcher block that is 60" x 30". I'm guessing that it's 40-50 pounds? Maybe more. This is probably WAY more weight than this leg set is made for. I also have the aforementioned keyboard tray that sticks out (torque equals force times lever arm) and causes even more torque. I get some shaking with the top with this setup. I have the desk on carpet; I was concerned that maybe the carpet and pad were not giving enough stability with the feet, so I made some plywood platforms for the feet. I also carefully leveled the feet to make sure it wasn't just uneven legs that was causing the instability. Still shakes no matter what I tried.
I think it's just that I have such a heavy top--more than this thing is spec'd for. That's totally fine, and I don't want to knock off a bunch of stars for that. I leave these comments more as a "don't do what I did" thing. Just be advised that you need to limit the weight of the top to something reasonable.
The design of one upright on each side of the desk with a pipe coupler in between is probably the weak spot with my heavy desktop; I'm thinking of either adding tension on either side (with some threaded rod and turnbuckles) or some extra supports with something like the Steel-Tek pieces from Lowe's.
Overall, I'm very satisfied with the product and the company!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 23, 2020
★★★★★ 5
So nice I bought twice!
Size: H Desk Unfinished 3/4-in and 1/2-in, Size: H Desk Unfinished 3/4-in and 1/2-in
For starters, this is a great buy if it is exactly what you want. For me that was the case and it would have cost me a good $40 or more to replicate it at a big box store. With that said, if you are not planning on using all of the pieces and want a different configuration, this may not be the kit for you. All of the parts inside are just like if you bought it at a local home improvement store. All the pipes need to be cleaned and protected. I personally found that dish soap (dry off immediately after you clean a piece) was the easiest to clean everything and I finished it with some wax. The kit is sturdy and easily supports the weight of my butcher block tops. I inverted the kit as it is designed as I intend on adding a shelf and am planning on using the support brace to aid in holding that shelf up for cable management. After cleaning and protecting, putting the kit together only took 5-10 minutes and that was with kid disruptions. I purchased 2 so that my wife and I could have matching desks in the office and I couldn't be happier.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 23, 2019
★★★★★ 5
Durable and sturdy. I bought two
Size: H Desk Unfinished 3/4-in and 1/2-in
Love the kit. I ordered two to make an L-shaped desk for my home office. Perfect height, looks cool, and sturdy. Slightly tricky to get two of them to the exact same height but you can adjust them buy loosing/tightning parts. Way cheaper than going to the hardware store and buying the same parts. I wiped each piece down as they are a bit dirty/greasy, but no big deal.
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Reviewed in the United States on August 9, 2021
★★★★★ 5
Great legs - sturdy when fully assembled with tabletop.
Size: H Desk Unfinished 3/4-in and 1/2-in
These legs are great. I put it together with a butcher block tabletop and it turned out beautifully. I recommend using a level on the legs once you get them into position and again when you place a top on it. The screws included were so easy to screw into the block but that will vary depending on the top you select. Watch the videos another reviewer posted on cleaning the legs with a mild soapy water and dry them thoroughly. It really helped me. Also after it was all assembled I used a clear acrylic - in a spray can to clear coat and seal the legs. I am really happy with it.
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Reviewed in the United States on July 4, 2020
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