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Description
Mouse HDAC2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a histone deacetylase 2 (HDAC2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of histone deacetylase 2 (HDAC2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Histone Deacetylase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Histone deacetylase 2, also known as HDAC2, is an enzyme encoded by the HDAC2 gene. It belongs to the histone deacetylase class of enzymes and is responsible for removing acetyl groups from lysine residues in the N-terminal regions of core histones (H2A, H2B, H3, and H4). As such, it plays a crucial role in gene expression by promoting the formation of transcriptional repression complexes and is therefore often considered an important target for cancer therapy. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
Immediately yes
Fill Material: Down Alternative, Size: King Size
I’m 50 and peri has been kicking my butt lately, so temperature management of my head (as that is the only thing effected by my hot flashes) has been an ever pressing project that I’ve been managing for the last 3-4 years. Hot sleeping has always been one of my curses, so adding perimenopause to the mix has been my own special hell. But Dyson fans, keeping the bedroom window cracked, and lots of all cotton gauze blankets have been imperative for me in the night. Pillows have always been pillowing, they just get warm and flipping them all night long is just finding the least hot version. I even keep 2 pillow choices on the bed while I sleep, and 2 more an arm’s length away on the chest at the bottom of the bed. Silk pillowcases are great for my hair and skin but they don’t keep cool. THESE PILLOWS, complete with the Beckham cooling pillowcases are chef's kiss. They feel like they’ve been in the fridge. Literally. Now do they stay that way, of course not. But they’re cool enough for me to fall asleep. And if I wake, I use the other side. And if I keep another on standby, I am literally finding the cool side of the pillow all night long. I’m not exaggerating either. These pillows are cool WITH the cooling pillowcases. You can’t use a pillow protector though, even if it’s cotton. It will ruin the effect. And you must use the cooling pillowcases. Do both, and you won’t be disappointed.
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Reviewed in the United States on March 9, 2026
★★★★★ 1
Terrible update to a good pillow
Fill Material: Down Alternative, Size: Queen/Standard Size
Purchased this as I was looking to replace tbe previous Beckham hotel pillows I had purchased before. When I saw "newer model available" I ordered it. Wish I had just ordered the same ones as before. First off this was only 1 pillow, not the 2 pack. Since I was only replacing 1 pillow I decided to use it. Huge mistake, my neck has been sore almost everyday. The support of this pillow is terrible, even with trying to added and remove some fill to adjust it. Nothing seems to make a difference. Avoid it and order a different model. Wish I had sent it back for a refund when I got it.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
Best Pillow e
Fill Material: Down Alternative, Size: Queen/Standard Size
I’ve been using this pillow for 3 months now. It is, hands down, the BEST pillow I’ve ever had. It’s soft and supportive at the same time. As I noted, I’ve been using it for 3 months and it has maintained its loft unlike some of the other types of pillow I’ve tried. Well worth the price and I highly recommend it.
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Reviewed in the United States on June 8, 2026
★★★★★ 5
Just perfect. Well made, No Issues Just one perfect sleep zzzzzzzzzzz😴
Color: White, Size: Queen (Pack of 2)
I had down pillows, and foam pillows, and other synthetic pillows etc and some were ok and others were not. I cannot get a great nights sleep if I am constantly having to adjust my pillow during the night. I ordered these pillows as I sleep on my side mostly but sometimes on my back. When they arrived I followed the directions (as they are in a vacuum pack) and only takes a few minutes for them to acclimate. Then put pillow cases on them and off to bed. I had the BEST sleep I have had in YEARS !!!!! The pillows are great. They keep your head comfortable and cool (No Sweating) and super comfortable. The only issue is after a perfect nights sleep, I find I really do not want to get out of bed in the morning..One great thing, is I am retired so I really do not have to get up out of bed until I want to 😴😳. The pillows are well made and feel like expensive down filled pillows but softer. I am VERY satisfied with the pillows. I just wish I would have found these pillows years ago. But now I have them and the perfect sleep that they provide. Dr Dave
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Reviewed in the United States on June 4, 2026
★★★★★ 5
Great sleep
Color: White, Size: Queen (Pack of 2), Color: White, Size: Queen (Pack of 2)
Finally found a pillow that is comfortable. Tested about 5 until I found the right one. This is perfect for back and side sleepers. Firm, but soft. Just enough filling and great quality. Will hold up for many years.
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Reviewed in the United States on May 24, 2026