Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 8 - Jul 13
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse SLC30A6 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Solute Carrier Family 30, Member 6 (SLC30A6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute Carrier Family 30, Member 6 (SLC30A6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Solute Carrier Family 30, Member 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Zinc transporter 6, also known as ZNT6/SLC30A6, is an enzyme encoded by the ZNT6/SLC30A6 gene. This gene encodes a member of the zinc transporter protein family. This protein regulates subcellular zinc levels in the Golgi apparatus and vesicles. Its expression is altered in Alzheimer's disease brain plaques. Diseases associated with SLC30A6 include autosomal dominant spastic paraplegia 4 and zinc-deficiency acrodermatitis enteropathicum. Pathways involved include metal ion SLC transporters and peptide hormone metabolism. An important paralog of this gene is SLC30A5. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 1737 reviews
Sort
Product Reviews
★★★★★ 4
One of the Few Fuel Additives I Keep Buying
Color: Pack of 2
I've been using this product in my 2015 Ford F-150 for more than five years, and it's one of the few fuel additives that has earned a permanent spot in my maintenance routine.
I originally started using it as preventive maintenance, but over time I noticed the engine seemed to run smoother after treatment. It's not the kind of product that suddenly turns your truck into a race car, but it has consistently done what I hoped it would do.
I've purchased and used it multiple times over the years, which probably says more than anything else I could write. If a product doesn't work or isn't worth the money, I usually don't buy it again. This one keeps finding its way back into my shopping cart.
I trust the product, I've had good results with it, and I think it's a reasonable value for the money. Based on my experience, I'll continue using it as part of my regular maintenance routine.
If you believe in taking care of your vehicle before problems start, this has been a worthwhile purchase for me.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026
★★★★★ 5
Mechanic Recommends Sea Foam -- Best Gas Treatment for All Engines
Color: Pack of 1
We have a small-engine mechanic who turned us onto SeaFoam motor treatment -- and against most gasoline preservatives and treatments. He said he'd drained, or seen inside, thousands of gasoline tanks, and the stuff sold as gasoline storage additives was often still there, sitting as gel on the bottom of the tank. Just gunk. He also said most brands of engine treatment weren't worth the money. However, he swore by SeaFoam, which I'd never tried at the time, and my experience since has proved him out. I've had many rough-running engines find their power and performance again, with nothing different, but a shot of SeaFoam in the gas tank (and the crankcase).
We own a number of vehicles and equipment with small engines, from a compact backhoe, to a ride'em Husqvarna mower, weedwacker, chainsaw -- and on the larger size, a Honda SUV and Ford F150 pickup. All of them have benefited greatly from the regular use of SeaFoam. However, when we're running the road vehicles a lot, especially in the summer, we go through gas and don't necessarily add SeaFoam with every fill. Recently, the Ford began to run rough. It stalled as I pulled up to corners and lights. In the past, that would have meant a mechanic and an expensive tune-up. All I did was go for a fill-up, and started by adding a full can of SeaFoam. Problem solved, just like that.
The Terramite (made in the USA) backhoe is not used a lot -- and as with most motors, that is a problem. It's bad for an engine not to run it -- and especially, in most cases, for it to sit with gas in the tank. The backhoe has a Kohler Command 25-horse gasoline engine that powers the hydraulics that run the wheels, steering, front-loader, and backhoe. I change the spark plugs each season, but it, like my Stihl chainsaw, and 6500 watt backup (gasoline) generator, and for that matter, all the small engines, can be hard to start after a hiatus. And even when they get going, they'll run rough. I used to drain the tanks frequently, then run them dry, then fill again before use, and hope for the best. But SeaFoam has changed all that. Throughout the summer, I can leave treated gas in the tank, and have little problems with starts or rough running.
In the smaller equipment, I dose the gasoline with each fill (1 oz per gallon), as SeaFoam cleans both carburetors and full injection systems. It is good for gasoline and diesel engines. Added to your crankcase (1.5 oz per quart of oil), it cleans deposits and quiets noisy lifters.
These days, most gasoline is mixed with ethanol and that's a bad thing for small engines. Ethanol goes hand in hand with water -- and as we all know, water in the gas is anathema to motors. Again, my small engine mechanic told me, 90% of the no-start problems he had to deal with were caused by ethanol-caused water in the fuel system. SeaFoam actually controls moisture and prevents this problem. It also de-ices and is anti-gel.
In my opinion, when it comes to gasoline and diesel engines, large and small, SeaFoam is THE Silver Bullet. My equipment is often running, while my neighbors are running with theirs to the shop. I've told many about SeaFoam, but some people seem to want to do it the hard way.
By the way, I also swear by PRI-G fuel additive for long-term gasoline storage. I keep 100 gallons of gas in tanks for emergency use. We are in a rural, wilderness area. I was introduced to that by survival expert, Steve Harris. He said he's had gasoline that was 10 years old still run equipment, because he'd added PRI-G to it (you can buy here on Amazon). Of course, you have to remember to add more PRI-G each year -- that's the catch.
So our rule of thumb is, PRI-G for long term fuel storage -- and SeaFoam for month to month. Also -- this price is right! Of course, when you compare the cost of equipment breakdown, and having to take anything to a mechanic, this is almost free. However, apples to apples, this price is cheaper than Walmart -- and up to $4 cheaper per can than many auto supply and hardware stores.
Can't say enough good things about this product! Highly recommended.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 19, 2015
★★★★★ 5
I swear by Seafoam, especially for small engines
Color: 3 Pack
I swear by Seafoam. It has really helped out my small engines, especially the 2 strokes and ones that sit around for long periods of time like my leaf blower and roto tiller. After I started adding Seafoam every time I get fuel I have had zero starting issues. They can sit all year and they will still start right up. In my car/trucks with higher miles I've used it to clean out the crankcase with great results. I add a can to the oil right before doing an oil change. I add a can then take a short drive(10 miles) to make sure the oil is up to operating temperature and change the oil once I get home. It does a good job cleaning any sludge out of the pan and the new oil stays looking fresh/clean way longer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
It's Sea Foam (but not green)
Color: 3 Pack
What is there to say? If you're buying Sea Foam, you know what it is and what it does, and you're probably going to continue buying it. It's great stuff.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 21, 2026
★★★★★ 5
Quality Champion Brand plugs
Repeat customer. Quality Champion product worth the extra $
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026