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Description
Human HSL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed immediately or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. 3. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Hormone Sensitive Lipase (HSL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Hormone Sensitive Lipase (HSL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Hormone Sensitive Lipase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hormone-sensitive lipase (HSL), formerly known as cholesterol ester hydrolase (CEH) and sometimes referred to as triacylglycerol lipase, is an enzyme encoded by the LIPE gene. HSL is an intracellular neutral lipase capable of hydrolyzing a variety of esters. The enzyme exists in two forms: a long and a short. The long form is expressed in steroidogenic tissues, such as the testes, where it converts cholesterol esters into free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, where it, among other things, hydrolyzes stored triglycerides into free fatty acids. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.1 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 4
Nice partition for my basement
Color: Beige, Size: B-102''-3 Panel
This 3-panel room divider showed up well-packaged with all the parts and even a little tool for putting it together. The instructions were easy to follow, and while one person can definitely assemble it, having two makes it quicker and less frustrating.
It does exactly what I needed—gives quick, easy coverage in an open area. I'm using it in my basement to hide the furnace and water heater, and it works great for that. It looks nice overall, but I picked the beige fabric (which is really more of a tan), and the black frame makes it stand out more than I’d like. A white or lighter frame would’ve blended better. Not sure that I would use it anywhere besides the basement
It’s lightweight and pretty sturdy. The wheels make it easy to move, but it does get a bit wobbly, and the panel clips need to be readjusted every time you roll it somewhere new. Still, for the price, it’s a solid buy. I’m happy with it and planning to order another one.
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Reviewed in the United States on June 21, 2025
★★★★★ 5
Fantastic
Color: Beige, Size: A-132''W-6 Panel
I am perennially in the process of rearranging, reorganizing, getting ready for yard sales, changing the functions of various rooms, moving everything out of a room to do a deep clean, etc, which means that there is always SOME part of my house, if not the entire thing, that is a disaster zone. Having someone knock on my door triggers a "duck and cover" reflex because it's so embarrassing for anyone to see that I live like this. (I *feel* like I don't, really, because I know that all the various stages are all temporary, but when there's always something temporary going on, it might as well be permanent, you know?)
So THANK HEAVENS for a room divider that is not only large enough to divide my uncomfortably long den in half the long way so I can shove all the messy parts up against the back wall -- that is not only simple and attractive and neutral and (this is important) NOT BLACK because I have multiple pets and anything black in this house will be pet-hair-colored within 20 seconds -- that not only has **SIX** panels for lots of options of how I want to divide the space/s -- but is also on WHEELS.
Someone out there really does love me, it's true!
The panels are stable *enough* - I'm not going to lie and say that nothing is going to knock them over, because I have some balance issues, and I also have cats, and both of those circumstances has resulted in a mess on at least one occasion. But in the cats' defense, they've never dealt with a room partition that didn't hang from the ceiling before; and in my defense, I just grabbed the first thing I could reach on the way down. Not the product's fault in either case.
Assembly was a little dicey as I am currently in **bilateral** (oh yes, that's fun) wrist braces, so I had to give up until I had help. But I think if you're able-bodied, you shouldn't have any difficulty.
The only down side is, now that I've hidden away all the mess so I can break out the holiday decorations, you know what happens next, right?? That's right... the PERMA-MESS ZONE. Oh no.
Oh well.
Full item name: Room Divider 132''W Wall Divider for Room Partition (Heavy Duty Base) 6 Panel Partition Room Dividers, Freestanding Room Divider Screen Separation W/Wheel Folding Privacy Screen Panel Space Separator
(six panels, beige)
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Reviewed in the United States on December 1, 2025
★★★★★ 3
Difficult assembly, doesn’t fold well, looks good fully open
Color: Grey, Size: B-88''W-4 Panel
The instructions for this divider are very inadequate. After having to redo parts of the assembly, I have finally assembled it. The divider itself seems very nice when it is fully open, however it is not possible to fold it well as all the bars with the wheels go in various directions making it impossible to fold it neatly. And it has a large footprint. It is possible that I did not assemble it fully well because of the lack of adequate instructions. I am not even sure if I will keep it because I need to fold out of the way when nothing in use.
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Reviewed in the United States on April 16, 2026
★★★★★ 4
Slightly challenging build
Color: Beige, Size: A-88''W-4 Panel
This was my second screen set I've purchased, first of this style. Not the easiest to assemble. Lots of pulling and tugging; not a highlight in my day.
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Reviewed in the United States on January 15, 2026
★★★★★ 5
Commercial grade divider suitable for many needs
Color: Beige, Size: A-132''W-6 Panel
This item will be used at a future event where we need to separate a single locker room into two large areas, without any construction. This divider is perfect for the job because the partitions can be 1 to 6 panels and it will roll into place. When the event is over, the divider will fold/roll away and the locker room will return to its original design.
The unit is very heavy, which will ensure that it is sturdy and stable. All the components arrived, wrapped in plastic to prevent damage. The screen material is a woven polyester in a cream color that will occlude any visibility but it may be possible to see shadows on the opposite side. The height is about 6ft, which would require a very tall person to "peep over" the divider.
I needed a quality unit for this project and the frame, screens, and mobility will meet the needs. The divider is not the same as the small decorative dividers -- it is more of a commercial grade product, yet tasteful. If you want/need a small divider as an accent piece, this is not the best choice. This item could be used in a medical office, treatment/massage room, to divide an office room into two, or block a hallway in a building.
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Reviewed in the United States on December 2, 2025