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Description
10x Kinase Buffer CProduct Specification Physical Appearance Liquid Stability & Storage For long term storage, keep 10X buffer at 20C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4C for 1 2 weeks. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze thaw cycles. Background Kise Buffer can
Product Specification
| Physical Appearance | Liquid |
| Stability & Storage | For long term storage, keep 10X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable |
Background
Kise Buffer can be used to assay protein kise activity.
Components
250 mM Tris-HCl (pH 7.5), 50 mM beta-glycerophosphate, 20 mM dithiothreitol (DTT), 1 mM 3VO4, 100 mM MgCl2
Protocol
1. Thaw 10X buffer at 24-30°C, mixing end-over-end.
2. Dilute the 10X buffer to the desired concentration using ddH2O.
Guidelines
Kise Buffer must be supplemented with ATP.
Shipping Notes
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Exchange/Return Notes
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