Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 10 - Aug 15
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TNFAIP8L2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tip: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: serum, plasma, tissue, homogenates, cell culture, supernatants and other biological fluids Preparation before testing: 1. Please take out the test kit from
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tip: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: serum, plasma, tissue, homogenates, cell culture, supernatants and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against TNF alpha-induced protein 8 like 2 (TNFAIP8L2). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of TNF alpha-induced protein 8 like 2 (TNFAIP8L2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TNF alpha induced protein 8 like 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | TNFα-induced protein 8-like 2 (TNFAIP8L2), also known as TIPE2, is a protein encoded by the TNFAIP8L2 gene. It is preferentially expressed in myeloid cell types and acts as an immune checkpoint regulator of inflammation and metabolism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 12 reviews
Sort
Product Reviews
★★★★★ 5
Delicious and Healthy Snack with Unmatched Flavor and Texture
Flavor Name: Traditional, Size: 4 Ounce (Pack of 1)
As someone who is constantly on the lookout for tasty and healthy snacks, I recently came across these Droewors Beef Sticks, and I have to say, I'm truly impressed. The unique air-drying process, combined with the use of vinegar, cloves, and coriander, gives these beef sticks a fantastic flavor that is not only mouth-watering but also worlds apart from other meat sticks I've tried in the past.
Pros:
Unique Flavor - The combination of spices and the air-drying process creates a taste that is both savory and satisfying.
Texture - The coarse ground beef provides a buttery smooth texture that doesn't leave your hands greasy, unlike other meat sticks.
Keto, Paleo, and Whole 30 Friendly - These beef sticks fit well with a variety of diets, making them a versatile snack option.
High Protein Content - With more protein per ounce than other meat sticks, they're a great natural alternative to protein shakes for muscle fuel.
No Sugar - The absence of sugar means no sugar rushes or mood swings, making it a great option for a healthy diet.
Cons:
Price - These beef sticks might be a bit more expensive than other brands due to their high-quality ingredients and production process. However, I believe the unique flavor and health benefits make the price justifiable.
In conclusion, these Droewors Beef Sticks are a delicious and healthy snack option for those following Keto, Paleo, or Whole 30 diets, or simply looking for a tasty protein-packed snack. The unique flavor and texture, coupled with the high protein content and absence of sugar, make these beef sticks stand out from the crowd. While they may be a bit pricier than other meat sticks, the quality and taste make it well worth the investment. Highly recommended!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 22, 2023
★★★★★ 5
Good Food
Flavor Name: Traditional, Size: 4 Ounce (Pack of 1)
I have had South African Billtong before and I loved it, so I thought I would try SA Droewars and see if they were as satisfying. The answer is yes! They are delicious and nothing like your typical mass marketed jerky snacks sold in the US. Droewars are lightly seasoned beef jerky sticks and, although I have not seen the numbers, they seem to be only lightly salted (important for me). The meat is not chewy or grisly, so the beef cuts they use must be prime.
This is the first time I have tried the Ayoba-Yo brand and I am happy with my purchase. I am awaiting delivery of several varieties of Billtong from Ayoba-Yo presently and I will report back.
Not inexpensive, but the value is there for sure.
Jimmy
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 17, 2019
★★★★★ 1
Almost not edible
Flavor Name: Traditional, Size: 2 Ounce (Pack of 3), Flavor Name: Traditional, Size: 2 Ounce (Pack of 3)
I am south african, an afrikaaner. I grew up eating droewors and biltong. I'm not sure what went wrong with this product. I have to believe it was off quality product and not what they are choosing to ship. The flavor was ok. Nothing spectacular. Thr big issue was texture of the droewors and the fact that the meat had shrunk away from the casing leaving the casing inedible. See my pic. This is not what droeworse looks like. Also, being from south africa I've never heard anyone say the word ayoba....
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 28, 2023
★★★★★ 4
Good taste. A bit dry texture.
Flavor Name: Traditional, Size: 4 Ounce (Pack of 1)
Great taste. Unusual flavor but still good. It was a very dry texture. I do want to try another flavor.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 19, 2025
★★★★★ 3
Meh
Flavor Name: Traditional, Size: 2 Ounce (Pack of 3)
It’s just okay. I’ve been looking for something that is as close to possible as dry wors in South Africa. This is not it. Is it dry wors? Yes. But is it good dry wors? No; it is very average. I wouldn’t buy it again. I’ll have to keep on searching for that yummy, moist, soft, flavorful dry wors that seems so elusive on this side of the pond.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026