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Description
Mouse HDAC2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a histone deacetylase 2 (HDAC2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of histone deacetylase 2 (HDAC2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Histone Deacetylase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Histone deacetylase 2, also known as HDAC2, is an enzyme encoded by the HDAC2 gene. It belongs to the histone deacetylase class of enzymes and is responsible for removing acetyl groups from lysine residues in the N-terminal regions of core histones (H2A, H2B, H3, and H4). As such, it plays a crucial role in gene expression by promoting the formation of transcriptional repression complexes and is therefore often considered an important target for cancer therapy. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
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Product Reviews
★★★★★ 5
Fantastic performance with respect to privacy and room temperature.
Color: Frost
While this standing room divider is tall and wide enough to provide a nice privacy barrier, it also allows light to filter through while maintaining that privacy. Assembly was easy with simple to follow directions and the only place I struggled was in pulling the fabric down to connect it. Similar to putting on a compression sock- it takes time and stretching/pulling. I don't think I ever got it to line up completely at the bottom and velcro together like it should, but it is a fantastic benefit. I have a home office on the ground floor, so when the curtains are open, it's not entirely unexpected to have a passerby look in as they go past. This provides basically a wall for them to look at. And I love it. Aside from the privacy, an unexpected benefit is wind control. On hotter days, my dog likes the window open, but hot to him may be cold to me, so I open it for him and allow this instant wall to shelter me. It is surprisingly effective at keeping the cold breeze from reaching me. I could not be more impressed with this divider. It stands on its own and provides privacy and temperature regulation through it's sturdy frame and thick (light-friendly) fabric.
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Reviewed in the United States on November 14, 2025
★★★★★ 5
Hiding from Coworkers Has Never Looked So Classy
Color: Frost
Let me tell you something: this divider isn’t just a piece of furniture...it’s a sanctuary, a confessional booth for the emotionally exhausted, and a glorious wall between me and other people’s nonsense. I slid behind this beauty like a barfly dodging an ex, and baby, I felt safe.
It's lightweight, but sturdy enough to block out both sunlight and bad vibes. The fabric’s got a fancy little texture, which is great if you're hiding and still wanna feel aesthetic. It moves easy, it stands tall, and it gives me just enough space to eat my sad desk lunch in peace while pretending I’m in a better timeline.
Final Word:
Perfect for offices, bedrooms, dressing rooms, or wherever you need to spiritually disassociate. Whether you’re blocking out sunlight or Barbara’s unsolicited MLM pitch, this thing’s got your back. Literally.
Five stars. Would (and likely will) emotionally withdraw again.
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Reviewed in the United States on October 28, 2025
★★★★★ 5
Great quality, perfect height to block the sun from the skylights.
Color: Frost, Color: Frost
We have skylights in our home that don't have shades yet so when we are in our loft, the sun will either blind us or make it unable to see our monitors clearly. So while we're waiting on installing shades, we thought to get a cover to block out the sun and this is perfect! It's tall enough to block out the sun and easy enough to move around as the sun moves. I don't love the way the cover looks, but it seems like it might be changeable. I think for privacy when we have guests in the loft, we would need a few more of these. The base is very sturdy and the quality overall is perfect. It is pricey, but most of these panels are. So really happy with this divider!
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Reviewed in the United States on October 10, 2025
★★★★★ 4
Works great but lacking value
Color: Nocturne
It's a well packaged item including a simple tool set and a clear instruction. No smell. I put it together just by myself in about 15-20 minutes without any problems. The base is solid pieces of metal, and it stands very sturdy. It's also light enough for me to move it around in my office to serve different purposes. I like the color and the quality of the fabric. It blends in with my office space perfectly. I needed it as a partition between my desk and the door, and the background for my video conference call. Surprisingly, it can also deflect the air coming down from the AC vent behind my desk. I didn't expect it to 100% soundproofing (not its purpose), but I feel it did absorb some humming sound from my mini fridge behind my desk. Sturdy metal for free-standing, yet pretty light weight for easy assemble and to move around. Keeping it beside my desk to separate lounging and work area, and I could move it easily if I want a conference-call backdrop. I can tell some difference in the sound absorption, as my all-in-one space (kitchen, dining, work, living) area can sound loud at times, it does provide some damping effect. But I must say for the price I was expecting more. More solidity and weight. More privacy. It's just not very big for the price paid. A good price for two panels but this was just a single.
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Reviewed in the United States on October 5, 2025
★★★★★ 4
High quality divider, but at a high cost
Color: Nocturne
So if you've ever been into an office setting you've likely encountered some variety of this divider. There's a bunch of good things about this product.
-It's a nice size
-There are great color options, went with Nocturne and no regrets with that
-It's sturdy with a good base
-Offers a bit of sound deadening
The biggest drawback of this product is the cost. It's pricey for ONE divider. I'm sure it would make a great set, although for me it would fiscally irresponsible.
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Reviewed in the United States on October 3, 2025