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Description
Mouse Intestinal Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) put the normal intestinal tissue of mice after sampling into the sampling bottle of pre cooled (2 8 c) tissue preservation solution e, and quickly transfer it to the clean laboratory for tissue processing and stem cell separation, take photos and register information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue
Product Specification
| Usage |
1. Primary (1) put the normal intestinal tissue of mice after sampling into the sampling bottle of pre cooled (2-8° c) tissue preservation solution e, and quickly transfer it to the clean laboratory for tissue processing and stem cell separation, take photos and register information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of small intestine tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 1-3mm3 (4) the tissue was digested with the primary tissue digestive solution c of mouse normal intestine, and then it was shaken at 4 ℃ for 10min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add mouse normal intestinal organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organ collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of mouse normal intestinal organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse normal intestinal organoid culture medium A. |
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| Description |
Composition:
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| Storage Temp. | 4 ℃, 3months-20 ℃, 1 year, see the label for details |
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4.9 ★★★★★
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Product Reviews
★★★★★ 1
Dnt buy!!
Flavor Name: Chicken Chips, Size: 1 Pound (Pack of 1)
These Suck! I have 3 Shih Tzus the one that ate it had a hard time chewing it and the other 2 couldn’t even I had to stick my finger down their mouth cuz they were choking and were trying to spit it out these are not chips they’re more like hard jello the texture is terrible even the color was weird the quality of this product sucks
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Reviewed in the United States on December 3, 2025
★★★★★ 5
Little Dog LOVED It
Item Shape: Ring, Size: 6 Count (Pack of 1)
These are very small but perfect for little dogs. My terrier mix is 17 lbs and got frightened by his bully stick hitting the floor several years ago. Since then he has refused to chew on every kind of chew I've bought him, despite using every method of convincing him they're safe.
This turkey tendon ring was the FIRST chew he's been willing to chew! He was trembling through the first few nibbles and then devoured the whole thing within 20 mins. I could not be happier and will buy more when he finishes this bag.
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Your dog will thank you
Item Shape: Ring, Size: 6 Count (Pack of 1), Item Shape: Ring, Size: 6 Count (Pack of 1)
My dog is obsessed!! I don't even think obsessed is the right word, like she will take a turkey ring over going to the dog park! She has a sensitive stomach and I love that these turkey treats don't give her diarrhea or gas. I work from home so when I have an important meeting or something that requires my undivided attention, I give her one of these and it keeps her occupied for about 30 to 40 mins. They are a bit pricey but my little princess is worth it.
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Great treats
Item Shape: Ring, Size: 6 Count (Pack of 1)
My puppy LOVES these! He can be in the other room and when I go to open the bag hes right behind me. He will do anything for these!
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Reviewed in the United States on May 24, 2026
★★★★★ 4
Good for puppies.
Item Shape: Ring, Size: 6 Count (Pack of 1)
Puppies chew on, very good for teething. Older dogs will try to eat them whole.
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Reviewed in the United States on April 24, 2026