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Description
Human lL-2 OneStep OptiQuant Plus ELISA KitProduct Specification Antigen IL 2 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied. Kit Precision Intra assay: 4. 4% Inter assay: 6. 2% Sample type Serum, Plasma, Cell culture supernatant Assay type Sandwich (quantitative) Sensitivity 1. 990 pg ml Range 3. 13 pg mL 200 pg mL Recovery Cell culture supernatant: 96%
Product Specification
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay:4.4%； Inter-assay: 6.2% |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.990 pg/ml |
| Range | 3.13 pg/mL – 200 pg/mL |
| Recovery | Cell culture supernatant: 96% Serum: 111% Plasma: 92% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
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