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Description
LIF Protein, RatProduct Specification Species Rat Synonyms Leukemia inhibitory factor; Differentiation stimulating factor; lif; D factor Accession P17777 Amino Acid Sequence Ser23 Phe202 Expression System E. coli Molecular Weight 18kDa (Reducing) Purity 95% by SDS PAGE and HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris, 200mM NaCl, 1m DTT, pH8. 0 Reconstitution Reconstitute at 0. 1 1 mg ml
Product Specification
| Species | Rat |
| Synonyms | Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor |
| Accession | P17777 |
| Amino Acid Sequence | Ser23-Phe202 |
| Expression System | E.coli |
| Molecular Weight | 18kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE and HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, 1m DTT, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Ryffel B. et al. (1993) International Review of Experimental Pathology. 34: 69-72. |
Background
The leukemia inhibitory factor (LIF) belongs to the interleukin-6 (IL-6) cytokine family, which is involved in various biological processes including inflammation, immune responses, and embryonic development. Leukemia inhibitory factor is a glycoprotein growth and differentiation factor with pleiotropic activity. LIF has potent effects on the hematopoietic system, including megakaryocyte progenitor cells. In addition, LIF has bone regeneration activity, induces cachexia and acute-phase response in hepatocytes, and inhibits adipogenesis, to mention the more important activities. In vivo LIF treatment in monkeys and rodents was followed by signs of general toxicity, cachexia, acute-phase reaction, and stimulation of hematopoiesis. The safety margin for possible therapeutic effects on hematopoiesis seems to be very narrow.
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