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Description
Human RBM8A ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an RNA-binding protein 8A (RBM8A) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of RNA-binding protein 8A (RBM8A) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human RNA-binding protein 8A ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RNA-binding motif protein 8 (RBM8), also known as RNA-binding protein 8A (RBM8A), is a protein encoded by the RBM8 gene. This gene encodes a protein with a conserved RNA-binding motif. The protein is primarily present in the nucleus, although it is also found in the cytoplasm. It preferentially associates with spliced mRNAs, including nuclear mRNAs and newly exported cytoplasmic mRNAs. It is believed that the protein remains associated with spliced mRNAs, serving as a marker to indicate the presence of introns, thereby coupling pre- and post-mRNA splicing events. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Todo según lo esperado
Color: Dark Brown
Todo como lo esperado
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Great for blue collar guys
Color: Dark Brown
Perfect size for dumping pockets, phone, knife and keys after work or when switching pants
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Reviewed in the United States on March 3, 2026
★★★★★ 5
Well-built MacBook Stand to Help Eliminate Ergonomic Problems
Color: silver
Like so many people these days, a lot of my life revolves around being on my laptop. Like so many people these days, all that time hunched over a laptop was causing physical discomfort. Once I realized the source of my problems and reading up on solutions to common laptop ergonomics problems, I chose this laptop stand as one of my tools to correct the ergonomics issues facing me, and eight months later I am happy to inform you that this has done its job well.
Fortunately, I was already using my laptop on a standard-height desk, but having the screen at desk height (30" off the floor) was the major source of neck pain for me. At its lowest setting (the knob all the way to the left), it raises my 13-inch MacBook Pro about 5-3/4" off the table (measured to the bottom of the aluminum body that covers the back of the laptop screen). Note that if your MacBook has a different depth (measured from front to back), the amount your screen is raised will be a little different. At the highest setting (knob to the right), the screen is raised about 7-1/2" off the table.
I am just over 6' tall and tend to find that my neck is able to maintain a comfortable neutral position when the knob in the right third of the slide, but of course that varies according to each user, their sitting position, and own comfort zone. All-in-all, this provides a really great range of screen heights that is likely to fit most peoples' needs, especially on 30" height desk.
I think the pictures do a great job of covering aesthetics (that is certainly a big part of this purchase). It is high-quality aluminum, and like all aluminum, it is a fairly soft, scratch-prone metal. Mine has remained in nearly new condition after 8 months, and it maintains a clean look that looks almost looks like an Apple product. If you have one of the newer space gray or colored-aluminum MacBooks, that might obviously be an issue.
Mechanically, the stand is solid. There are no indents or notches in the slide. There is a slight spring to the upper "lever" of the base such that if you have the knob set to the lower positions, the upper part of the base springs upward to the highest position when the laptop is removed. In the highest position, the stand is already at its highest position, and there is no movement. I suspect that the spring is actually to help the upper arm move upward as the knob is slid to higher positions.
One caveat for use of this stand should be used with an external mouse and keyboard. Of course, Apple's Magic line of keyboards, mice, and trackpads work well with this, but the point is that when the laptop is raised, it is no longer comfortable (or ergonomic) to use the laptop's keyboard and trackpad.
Overall, this product delivers in every way I need. The price is on the high side, but I hope it will last through the life of this laptop and at least one replacement or three (and maybe even the days when the laptops become archaic relics of the past). The range of screen lift (about 5-3/4" to 7-1/2") suits me well (and I suspect, will suit a large number of people) and the aesthetics are a great match for MacBook users.
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Reviewed in the United States on October 16, 2017
★★★★★ 5
Very sturdy and sleek looking laptop stand
Color: silver
I was skeptical when I first ordered this. It didn't look very sturdy in the pics, but I was wrong. It is very sleek looking and functional, with the adjustable height. Very modern looking as well and my MacBook Pro fits perfectly on it. Very glad to have purchased this for my home office.
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Reviewed in the United States on March 2, 2024
★★★★★ 4
Sturdy, works great, not quite perfect.
Color: silver
This was the perfect stand to use when my desktop computer crashed and I had to work on my laptop without killing my neck. It's sturdy, well made, and works exactly as advertised. Adjustment is smooth and simple. I was able to work with my monitor at a comfortable height and distance, and it saved my bacon! But, would I want to work on it indefinitely? No. Two things are lacking for me. First, there's a height adjustment, but no angle control. This means that at its max height, the display has a small downward tilt. It also means that on zoom calls, I had to fiddle with the monitor height until my whole head was framed, rather than cut off. Not a dealbreaker, but annoying over the long term, especially with lots of zoom meetings. Second, I'm not sure why I was surprised, but the stand-plus-laptop combo created a kind of aluminum "monolith" on my desktop. I couldn't bend to peer under it, or over it either. It felt as though part of my desk (albeit a narrow portion) became inaccessible. This too is a quibble--I just happen to like a more open and airy feeling. These two nits aside, this is a sturdy and well designed stand that works well and will last a long time.
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Reviewed in the United States on May 27, 2026