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Description
Mouse TCF7 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Transcription factor 7 (TCF7) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the Transcription factor 7 (TCF7) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Transcription factor 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transcription factor 7 (TCF7) is a protein-coding gene. Diseases associated with TCF7 include gastric cardia adenocarcinoma and hepatocellular carcinoma. Pathways involved include the IL-2 pathway and amplification and expansion of oncogenic pathways that are characteristic of metastasis. Gene Ontology (GO) annotations associated with this gene include DNA-bound transcription factor activity and chromatin binding. An important homologue of this gene is TCF7L2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
LOVE this pillow!!!! I have 2!!!!
Size: Queen (Mid Loft), Number of Items: 1
Well…..I have a love affair with these latex pillows. I purchased 2 of them. I have been allergic to feathers since birth and could only use latex pillows growing up. But, they became very hard to find for many years. I tried all the other kinds of pillows on the market and hated them all!!! “Won’t go flat!”….. whatever! They would go flat almost immediately. Or “Good for side sleepers!”….. NOPE!! I had a stiff neck for a week!! DONE with all of them. So,I finally got on Amazon and found this pillow. Ahhhhhh!! Loved it. Slept like a log! All night! Didn’t go flat!! No stiff neck!!! A winner!!!! Frankly,I don’t care about the cost… yeah…I know….we call have to count our change……including me. I saved to buy this pillow…..both times. AND, I would do it AGAIN!!!! And…proud to do it! So, if you have had it with all of those new prefangled so-called pillows…try this one. I would only avoid it like the plague if you are allergic to latex. Otherwise,I would definitely recommend this pillow! Wherever I go….my pillow goes. Camping, on a trip, to visit relatives…..my pillow will travel!!!!
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Reviewed in the United States on October 3, 2025
★★★★★ 5
So comfy
Size: King (Pack of 1), Style: Standard Loft (4" - 5")
I spent time looking up/watching videos on pillows for neck pain and this one comes up a lot. I’m someone that used to wake up every few weeks with a stiff neck so bad it could make me cry at work. I haven’t had that happen once after months of using this pillow. I look forward to laying my head down on it every night. It’s a luxury experience every time. I think what it does so well is maintain its shape. You can really squish it up how you want and it stays there even after hours. Moldable but somehow still firm. It’s expensive but to me the value is there because I no longer get crazy neck pain from sleep. Worth trying it out for sure. Btw I was stuck between this is and Purple brands couple top end options. I never got around to trying them because I tried this one first and was very satisfied!
Feb 2026. Still use it every night and it's still protecting me from neck pain I used to get from sleeping. It's held up amazing. Hope to use it for many more years.
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Reviewed in the United States on January 18, 2025
★★★★★ 5
Saatva Pillow is great
Size: Standard/Queen (Pack of 1), Style: Standard Loft (4" - 5")
So far this is the best pillow I have had in the last 10 years and I have tried two Purple pillows a couple different memory foam and Gel pillows at least 4 more pillows that I can't even remember the names of. My neck and shoulders are almost pain free. I have also been doing more stretching of my arms which is also helping, but this pillow has really made the most difference in the pain I have had, I felt difference from the first time I put my head on it. It's comfortable, the perfect height I got the 4"-5", I also got the queen size and it's great. It supports my head just right. I also ordered the Saatva Classic 14" mattress from the company and I sure hope it's as good or better would be great because I have back issues for the last 50 years.
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Reviewed in the United States on September 16, 2025
★★★★★ 1
Cotton Fluff Around a Mostly Empty Bag
Size: King (Pack of 1), Style: High Loft (6"-7")
Bought the high-loft king, thinking it would be a great (tall) side-sleeper. What I got? A very nice, completely compressible cotton outer shell wrapped around a bag a bit smaller than a standard pillow. In that bag? A tiny amount of small latex chunks, just about a third full. That’s right, some cotton fluff around a basically empty bag. Could you buy some latex yourself and add it? No, the bag is sewn shut. You could probably fill a small pillow with latex you bought elsewhere and put that in. But seriously, what did you pay Saatva for in that case?
Probably as close as I’ve seen a major/high-reputation company get towards a literal example of rip off.
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Reviewed in the United States on May 31, 2026
★★★★★ 3
Wrong item ships/decent pillow/price unjustified
Size: Standard/Queen (Pack of 1), Style: Standard Loft (4" - 5")
Sent me the wrong pillow twice in a row. It said high loft and standard loft on the packing, not sure what the problem is. I ordered standard loft both times, and both times they sent high loft. That name is very deceiving though. If what I have is high loft, the standard loft wouldn’t be much thicker than your standard flat down pillow. If you’d like any sort of neck support I’d suggest starting with the high loft and going down instead of the other way around. I am 5’ 7” 130lbs and this pillow does not lift my neck up further than neutral, which is a good thing. Shredded memory foam pillows are significantly higher or thicker if that helps.
Pillow itself grew on me. You can definitely feel that there is a different material in the center of the pillow. I ordered it as a gift and ended up keeping both pillows. The price tag on this item is absolutely outrageous. I would have never ordered it for myself. Saatva products don’t last in my experience, we got their firmest mattress and within 2 years it was sagging horribly. I am interested to see how long this pillow lasts!
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Reviewed in the United States on January 19, 2026
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