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Description
Human E-Cad ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with E-cadherin (E-Cad) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of E-cadherin (E-Cad) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human E-Cadherin（E-Cad） | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cadherin-1, also known as CAM 120/80, epithelial cadherin, or uvomorulin, is a protein encoded by the CDH1 gene. Mutations in this gene are associated with gastric, breast, colorectal, thyroid, and ovarian cancers. CDH1, also designated CD324, is a tumor suppressor gene. E-cadherin is first expressed during mammalian development at the 2-cell stage and becomes phosphorylated at the 8-cell stage, leading to compaction. In adult tissues, E-cadherin is expressed in epithelial tissues, where it is continuously regenerated on the cell surface with a half-life of 5 hours. Cell-cell interactions mediated by E-cadherin are crucial for embryogenesis in many animals. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Lightweight cargo joggers with a lounge-wear feel, built for comfort and utility.
Size: 38, Color: Light Khaki
Lightweight cargo joggers with a lounge-wear feel, built for comfort, utility, and water-repellent performance.
Initial Wear & Feel Review: TACVASEN Men's Casual Cargo Joggers
This initial assessment is based on my first tactile experience and the manufacturer's details. A conclusive evaluation of durability, water repellency, and fit retention will follow extended wear across various activities.
Primary Specifications & First Impression:
These are tapered cargo joggers made from 100% polyester with a micro-elastic weave. My immediate takeaway is their exceptional comfort—they are so lightweight and soft they feel like loungewear, almost like wearing no pants at all. They feature a fully elastic waist with a drawstring, five utility pockets, and a water-repellent finish.
Notable Design & Comfort Features:
Unmatched Lightweight Comfort: The core achievement is a cargo pant that feels like lounge pants. The fabric's elasticity and minimal weight create a remarkable sense of freedom without bulk, living up to its "eases movement strain" claim from the first wear.
Practical Hybrid Design: They merge utility (multiple reinforced pockets) with a modern, tapered athletic silhouette (jogger style). This makes them viable for light hiking, travel, or casual work where traditional cargo pants might be too bulky or rigid.
Low-Maintenance & Weather-Aware: The water-repellent treatment and wrinkle-resistant, machine-washable polyester align with a practical, active lifestyle, needing minimal care while offering some protection against the elements.
Key Points for Future Performance & Durability Testing:
The most critical aspects I will verify through extended wear will be the fabric's long-term breathability and odor resistance, the durability of seams and pocket reinforcements under load, the effectiveness and longevity of the water-repellent coating, and whether the elasticity and fit remain secure after multiple washes. I will test these pants in casual, hiking, and travel scenarios.
For the final rating, I will return to report on the longevity and performance over time after thorough testing of their comfort durability, utility, and all-occasion versatility.
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Reviewed in the United States on January 16, 2026
★★★★★ 5
Awesome
Stylist
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Reviewed in the United States on December 18, 2025
★★★★★ 1
Why buy a dumb watch when you can buy a smart watch?
Just a watch. Doesn’t do anything else. Get something smart. The dumb watch industry makes no sense to me in 2026.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
The fit is tailored yet comfortable, accommodating a range of body type.
Color: All White, Size: 3X-Large
The **DEMEANOR Men's Dress Shirts** are a fantastic addition to any man's wardrobe, offering a perfect blend of style, comfort, and functionality. These **long sleeve, slim fit button-down shirts** are designed for both formal and casual occasions, making them incredibly versatile for any setting, from the office to a night out.
One of the standout features of these shirts is their **wrinkle-free fabric**, which ensures you look sharp and polished throughout the day without the hassle of constant ironing. Additionally, the **stain-repellent technology** helps keep the shirt looking fresh, as it resists spills from coffee, wine, and other common stains, making it ideal for busy professionals.
The fit is tailored yet comfortable, accommodating a range of body types, including big and tall sizes. This inclusivity ensures that everyone can enjoy the sleek look of a well-fitted dress shirt. The breathable fabric also adds to the comfort, allowing for ease of movement and all-day wear.
Overall, the **DEMEANOR Men's Dress Shirts** are an excellent choice for anyone seeking a reliable, stylish, and practical shirt. With their modern design and thoughtful features, these shirts are sure to elevate your wardrobe and keep you looking your best, no matter the occasion!
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Reviewed in the United States on October 8, 2024
★★★★★ 5
Great Quality
Color: Black, Size: Small
My son loves these shirts. We have them in several colors. They are so soft, light weight and wash great. He is in college and he can throw them in the washer and dryer and not have to dry clean them which is a plus for him. They are slim fit and hold up well. One he has had for 2 years and it still looks great. Great value for the money.
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Reviewed in the United States on August 22, 2025
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