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Description
Mouse TIMP3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Tissue Inhibitors of Metalloproteinase 3 (TIMP3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Tissue Inhibitors of Metalloproteinase 3 (TIMP3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Tissue Inhibitors Of Metalloproteinase 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Matrix metalloproteinase inhibitor 3 is a protein encoded by the TIMP3 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. Proteins encoded by this gene family are inhibitors of matrix metalloproteinases, a group of peptidases involved in extracellular matrix (ECM) degradation. Expression of this gene is induced by mitogenic stimulation, and this netrin domain-containing protein is localized to the ECM. Mutations in this gene are associated with the autosomal dominant disorder Thorsby's fundus atrophy. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Excellent
I will buy again this products!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2025
★★★★★ 4
They are literally not heavy
They are narrow for my face
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Reviewed in the United States on May 6, 2025
★★★★★ 5
Tropical Fringe Fun for My Summer Outfits
Color: Natural, Color: Natural
I am absolutely obsessed with this straw purse because it feels like a vacation in my hand. The first thing I noticed is how the raffia fringe has this playful half moon shape that looks like a little lion mane but in a very high fashion way. My daughter even joked that it looks like a giant fancy taco which makes it such a fun and unique piece to carry around. It is incredibly lightweight which is a huge relief because I do not want to carry a heavy bag when the weather is hot. Even though it looks compact on my mannequin, it is surprisingly roomy inside and fits my phone and all my daily essentials without feeling stuffed. The magnetic closure is quite strong so I do not have to worry about my things falling out while I am walking around a summer party or a night out.
The neutral tan and cream colors in the fringe make it very easy to match with almost any outfit I own. I really appreciate the small metal brand patch on the front because it gives the bag a more polished and expensive look than a typical straw tote you might find at a souvenir shop. One thing to keep in mind is that the raffia is very textured so you should probably be careful with delicate silk fabrics so it does not snag. It feels very sturdy and well made for something that is woven from grass and I love how the fringe adds so much movement when I am moving around. This bag is definitely going to be my go to accessory for the rest of the season because it is both stylish and very practical for my needs.
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Reviewed in the United States on April 2, 2026
★★★★★ 5
👜 Effortless Spring-to-Summer Clutch That Feels One-of-a-Kind
Color: Natural
👜 Effortless Spring-to-Summer Clutch That Feels One-of-a-Kind
I’ve been a longtime fan of Sam Edelman, especially because they actually make stylish pieces that work for real life. So when I saw this clutch, I had a feeling it would be good… but I didn’t expect it to hit *this* level.
This bag is a vibe. The raffia texture and that half-moon fringe give it such a unique, standout look without trying too hard. It feels like the perfect piece to roll right into spring and carry straight through summer. Think beach days, dinners out, travel nights, all of it.
What I love most is that it doesn’t look like everything else out there. It feels original, like you found something special instead of just grabbing another basic clutch. It’s lightweight, easy to carry, and the magnetic closure keeps things simple and secure.
It also pairs effortlessly with different outfits. You can dress it up or keep it casual and it still works.
Now let’s be real, it’s a designer piece, so it’s not cheap. But for the style, quality, and uniqueness, I do think it delivers strong value for money.
Five stars from me. This one’s coming with me everywhere this season ☀️
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Reviewed in the United States on April 12, 2026
★★★★★ 5
Super cute clutch!
Color: Natural
This is a beautiful clutch. I got the “Natural” color, and the tones are definitely subtle. I am already eyeing the Spicy one too, but for me, the natural color is just easier to match with outfits. I did laugh though because my nephew said it looks like a taco, but honestly that just makes me love it even more!
The material is extremely nice, it feels very premium and well made. I was a bit nervous because I have had cheaper bags with that shredded-style material that flakes and falls apart, but that is not the case here. It feels really well made. I cannot speak for long-term durability yet, but so far there is no shedding or anything like that. I also did not notice any loose stitching or threads at all, it just feels like a high-quality bag.
It has small magnets to keep it closed, which work nicely. There is not a ton of space since it is a clutch, but there is still enough room for my phone and a few small items, so it works well.
Overall, it is just a really gorgeous, well made bag, and I really do not have anything to complain about.
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Reviewed in the United States on April 2, 2026