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Description
Rat REN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Renin (REN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Renin (REN) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Renin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Renin, also known as angiotensinogenase, is secreted by juxtaglomerular cells. These cells sense changes in renal perfusion pressure via stretch receptors in the vascular wall. Juxtaglomerular cells are also stimulated to release renin by signals from the macula. The macula senses changes in sodium delivery to the distal tubules and responds to the decrease in tubular sodium load by stimulating juxtaglomerular cells to release renin. The macula and paraglomerular cells together form the paraglomerular complex. Renin secretion is also stimulated by sympathetic nerves, primarily through activation of β1-adrenergic receptors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
My all time fave sunscreen!
Scent: Fragrance Free, Size: 1.7 Fl Oz (Pack of 1)
This is the best sunscreen! Does NOT burn my eyes like 95% of other sunscreens do. Does NOT break out my sensitive skin, or my sister who has the most sensitive skin ever! It goes on smoothly due to its nice texture, does NOT feel greasy. Wears great under foundation as well. Thank you Coola for such a great formula at an affordable price. I have turned so many 'sunscreen snobs' into fan favorites of this as well. If you haven't tried it, you should!
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Reviewed in the United States on February 8, 2026
★★★★★ 4
good for non-white skin
Scent: Fragrance Free, Size: 1.7 Fl Oz (Pack of 1)
Moisturizing, non-sticky, works for my sensitive skin. It's transparent and doesn't leave a white cast on my light brown skin. Smallish size is good for travel and purse. It's a little shiny; wish it came in matte.
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Reviewed in the United States on March 21, 2026
★★★★★ 5
Very effective
Scent: Fragrance Free, Size: 1.7 Fl Oz (Pack of 1)
I love this sunscreen for your face. It doesn’t feel oily. It doesn’t stay white on your skin. It absorbs and it really protects it nicely. I put it on every morning before I walk my dog in the sun and I’m out for normally at least an hour so it’s a really nice sunscreen and I would definitely give it a try.
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Reviewed in the United States on February 22, 2026
★★★★★ 5
Lightweight, clean, and effective — worth the repurchase
Scent: White Tea, Size: 1.7 Fl Oz (Pack of 1)
This is my go-to daily sunscreen. It’s lightweight, non-greasy, and absorbs quickly without leaving a white cast. I love that it’s made with clean ingredients and doesn’t irritate my sensitive skin. The white tea scent is subtle and fresh. I’ve repurchased this several times now — it’s one of the few sunscreens I actually enjoy using.
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Reviewed in the United States on June 25, 2025
★★★★★ 5
Great replacement for my discontinued Epionce sunblock
Scent: White Tea, Size: 1.7 Fl Oz (Pack of 1)
I really hate it when companies discontinue great products, and even though it cost a fortune, I loved the Epionce mineral sun block for faces. When I realized it was gone, I looked at the Coola line because I like their other products. Found this face sunblock for $32 (this could be 1/3 of the Epionce, certainly 1/2) and have used it for the past few weeks playing golf. So far, so good! I'm not noticing any color, certainly no burn. No irritation, and the scent is quite mild, only noticeable when it's first applied (IMO, and I'm pretty sensitive to scents). I really like that Coola has the Hawaii reef-safe designation, which I think is very important for all sunscreens, even when we're not going swimming in the ocean. As a friend pointed out to me once, even if you don't swim in the ocean, you go home and take a shower and all those chemicals wash down the drain and make their way eventually through the system, so might as well just not use them in the first place! Yes, it's more expensive using products like this, but IMO it's worth it. I also wear sun sleeves these days so I don't have to use as much sunscreen when I'm out on the golf course. Editing this to say that this product has avobenzone, which is actually not so great and may be banned in Hawaii by next year. Hmm. Guess I'll keep searching!
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Reviewed in the United States on July 7, 2023