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Description
Rat PROC ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a protein C (PROC) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the protein C (PROC) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Protein C ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Protein C, also known as autothrombin IIA and coagulation factor XIX, is a zymogen whose activated form plays an important role in regulating anticoagulation, inflammation, and cell death, as well as maintaining the permeability of blood vessels in humans and other animals. Activated protein C (APC) primarily accomplishes these actions by proteolytically cleaving the proteins Factor Va and Factor VIIIa. APC is classified as a serine protease because its active site contains a serine residue. The zymogen form of protein C is a vitamin K-dependent glycoprotein that circulates in plasma. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.7 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
Finally… a dog chew that doesn’t become a homemade prison shank
Size: Medium
My dog has a real talent: turning every “tough chew” into a sharp, weaponized shard that could star in an episode of Locked Up: Canine Edition. But THIS chew? Absolute game changer.
For the first time ever, I can relax—no splinters, no pointy edges, no fear he’s secretly crafting a shiv under the couch. Just happy, safe chewing that actually lasts longer than five minutes.
It’s durable, it’s safe, it keeps him busy, and I no longer feel like I’m living with a furry little inmate planning an escape.
Highly recommend if your dog chews like he’s got nothing but time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 29, 2026
★★★★★ 4
Would buy again
Size: Small
Would have given 5 stars, but my dog was able to chew this down pretty quickly, so not the most durable. I do like that this is made with better material than other dog chews, so I would buy again.
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Reviewed in the United States on February 12, 2026
★★★★★ 5
he Durable, Natural Chew That Finally Gives Peace of Mind
Size: Large
Why It's a Top-Tier Chew:
Truly Safe Ingredients (Plant-Powered): This is the biggest selling point. The HonestChew is made from food-grade, plant-based cellulose, vegetable oil, and real antler bone powder—no nylon, no petroleum, and no plastic. This means I can finally relax when my dog is relentlessly gnawing, knowing the ingredients are safe.
Engineered for Safety AND Toughness: The material is specifically designed to flake rather than splinter like hard bones or crack like many synthetic chews. This makes it a much safer choice for dental health and digestive safety, even for the most aggressive chompers. It has the feel of a hard chew but with a safer breakdown.
Irresistible to Dogs: The chew is infused with real antler powder, giving it a natural, gnaw-worthy scent and flavor that keeps my dog engaged for long periods. The curved, ergonomic Infinity shape is excellent for dogs to grip securely with their paws, promoting a comfortable chewing posture.
Dental Support: The nubs and ridges on the surface aren't just for looks; they provide a gentle scraping action that helps scrub away plaque and tartar buildup on the teeth and above the gumline while the dog is happily playing.
Who Needs This?
If you have a dog that quickly destroys "indestructible" toys, swallows rawhide too fast, or causes you stress with the risk of bone splintering, the WOOF HonestChew Infinity is the perfect solution. It stands up to serious chewing and satisfies that innate gnawing instinct with confidence.
A highly recommended, long-lasting, and thoughtfully designed product that puts pet health first.
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Reviewed in the United States on October 22, 2025
★★★★★ 5
Great toy for super chewers!!
Size: Large
My dog is a super chewer and he enjoys these so much. I do too cause I feel like my money is actually getting spent well than on a plush toy he’ll destroy in two seconds. Definitely recommend these all the time for anyone who has that same issue with their pets. Very durable and perfect size for my big pit.
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Reviewed in the United States on June 2, 2026
★★★★★ 4
My dog loves this toy and she hasn’t chewed it up yet.
Size: Medium
This is a good chew toy for my aggressive chewer. I bought the large size for my medium size dog.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 5, 2026
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