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Description
Mouse CPR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000×g for 20 minutes, and collect the supernatant for analysis. Preparation for the Assay: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Cytochrome P450 Reductase (CPR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Cytochrome P450 Reductase (CPR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Cytochrome P450 Reductase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 5
Good value
Style: 2026 Curved 24" 180Hz
Awesome
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
★★★★★ 5
Incredibly smooth gameplay with a stunning display
Size: 32"
The Samsung 32" Odyssey G65B has been an awesome upgrade to my gaming setup. The QHD resolution looks sharp and detailed, and the 240Hz refresh rate makes everything feel unbelievably smooth. Fast-paced games look fluid with no noticeable blur or tearing.
The curved screen adds to the immersion, especially on a 32-inch display. It pulls you into the game without feeling gimmicky. Colors are vibrant, contrast is excellent, and the screen gets plenty bright for both gaming and everyday use.
Build quality feels premium, and the monitor looks great on the desk. If you’re serious about gaming and want high refresh rates with a large, immersive display, this monitor is absolutely worth it. It delivers exactly what it promises and then some.
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Reviewed in the United States on December 17, 2025
★★★★★ 5
A top-tier gaming monitor that exceeds expectations in every way!
Size: 27"
The SAMSUNG 27" Odyssey G65B is hands down one of the best gaming monitors I’ve ever owned. If there were an option to give more than 5 stars, this product would absolutely earn 7! From its stunning QHD resolution to the ultra-smooth 240Hz refresh rate, this monitor has redefined my gaming experience.
First, let’s talk about the display quality. The QHD resolution combined with HDR 600 creates incredibly vibrant and lifelike visuals. Colors are rich, blacks are deep, and the contrast is outstanding. Whether I’m playing fast-paced shooters or exploring immersive RPG worlds, the level of detail and clarity is second to none. The 1000R curve pulls you into the action, making gameplay more immersive and natural for your field of vision. After hours of gaming, I experienced less eye strain compared to flat monitors, which is a huge plus.
The 240Hz refresh rate and 1ms response time (GTG) make this monitor a dream for competitive gaming. The responsiveness is flawless, and I noticed an immediate improvement in how fluid gameplay feels. There’s no ghosting, screen tearing, or input lag, even in the most intense gaming sessions. It’s perfect for FPS games like Call of Duty or Valorant, where every millisecond counts.
One of the standout features is the built-in Gaming Hub. I was able to stream games directly without even needing my PC or console turned on—a convenience I didn’t realize I needed until now. The user interface is intuitive, and the hub is packed with options, making this monitor as versatile as it is powerful.
Another fantastic addition is the array of connectivity options. With multiple HDMI and DisplayPort inputs, along with USB ports, this monitor can handle all your devices with ease. I have it connected to my PC, PS5, and even a streaming device, and switching between them is seamless.
Build quality is excellent, with a premium feel that matches the performance. The monitor stand is sturdy and highly adjustable, allowing you to tilt, swivel, and height-adjust to find the perfect viewing angle. The sleek design and thin bezels add a modern touch to any gaming setup.
Setting it up was a breeze. The box was well-packaged, and the monitor itself was easy to assemble. Samsung’s attention to detail is evident even in the setup process. Delivery was also smooth and timely, ensuring everything arrived in perfect condition.
In conclusion, the Samsung Odyssey G65B is a masterpiece of gaming technology. It checks every box for gamers—stunning visuals, exceptional performance, and advanced features. It’s worth every penny and then some. If you’re serious about gaming, this monitor will elevate your experience to an entirely new level. I cannot recommend it highly enough!
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Reviewed in the United States on December 5, 2024
★★★★★ 5
Absolutely great curved monitor for his PC. Samsung, top of the line can't go wrong!
Size: 32"
Bought this for my son's birthday / Christmas present because they are so close together. I think it was only about three or $400. But it is the Samsung which is a top rate item. And it has a curved screen for better viewing. That's what he wanted so that's what he got! LOL he said it works greatly and you can adjust so many settings such as the brightness the sound and everything else. Good size too! 32 inches that's like a TV screen LOL
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Reviewed in the United States on April 3, 2026
★★★★★ 1
Worst interface on the market. A sadist designed it.
Size: 32"
Great screen. The absolute worst interface in the business. Who in their right mind would want their monitor to change inputs on a whim? Who would design a 6 step process to change that input back? Samsung did. I wish I had spent my hard earned money elsewhere. I waste more time fixing this stupid monitor then I do enjoying the games I play on it. It's nightmare fuel.
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Reviewed in the United States on May 15, 2026
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