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Description
PerCP-Cy5.5 Mouse Anti-Rat CD25 Antibody (S-R676)Product Specification Host Mouse Antigen CD25 Synonyms Peripheral myelin protein 22; PMP 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Cd25; Pmp22 Location Cell membrane Accession P25094 Clone Number S R676 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample SD Rat splenocytes treated 72h with 2. 5g ml Cona Purification Protein G Concentration 0. 2 mg ml Conjugation PerCP Cy5. 5
Product Specification
| Host | Mouse |
| Antigen | CD25 |
| Synonyms | Peripheral myelin protein 22; PMP-22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein (SAG); Cd25; Pmp22 |
| Location | Cell membrane |
| Accession | P25094 |
| Clone Number | S-R676 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD Rat splenocytes treated 72h with 2.5μg/ml Cona |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | PerCP-Cy5.5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Rt |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a 55 kDa type I transmembrane glycoprotein essential for immune regulation, forming part of the high-affinity IL-2 receptor complex (with IL-2Rβ and the common γ chain) that mediates IL-2 signaling in activated T cells, B cells, NK cells, and particularly in CD4+ regulatory T cells (Tregs), where its expression is critical for maintaining immune homeostasis and self-tolerance, and is dynamically upregulated upon T cell activation through transcriptional control involving NF-AT factors, while also existing in a soluble form (sCD25) that can modulate IL-2 availability and serve as a clinical biomarker in autoimmune diseases, infections, and cancers.
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Exchange/Return Notes
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