SKU: 78532156706

Rat IFN-γ High Sensitivity ELISA Kit

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Description

Rat IFN-γ High Sensitivity ELISA KitProduct Specification Description Testing Principle This kit uses double antibody sandwich ELISA. Specific anti rat IFN Capture Antibody is precoated on a high affinity conjugate plate. Standard and test samples were added successively into the well of the plate, and the samples were fully shaken and mixed and placed at room temperature for 2 hour incubation. The IFN in the samples bound to the solid phase antibody. Wash thoroughly to remove free and

Product Specification

Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-rat IFN-γ Capture Antibody is precoated on a high affinity conjugate plate. Standard and test samples were added successively into the well of the plate, and the samples were fully shaken and mixed and placed at room temperature for 2-hour incubation. The IFN-γ in the samples bound to the solid phase antibody. Wash thoroughly to remove free and unbound components, then add a biotinylated detection antibody and incubate. After washing again, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added. After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again. After washing again, TMB color substrate was added and incubated in dark at room temperature for color development. The depth of color reaction was positively correlated with the concentration of IFN-γ in the sample. The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.

 

Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Biotin labeled IL-17A detection antibody, Standard Diluent, Detection Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Wash Solution, Stop Solution, SA-HRP, Seal Plate Mask, and Instructions.

Sensitivity::0.26 pg/mL

Detection range:3.13-200 pg/mL

Recovery rate:73-137%

Storage:2-8℃

The standard curve:

Background:

Interferon γ (IFN-γ), a soluble dimer cytokine, is the only member of type II interferon.It is secreted by natural killer cells (NK) and natural killer T cells (NKT) and plays a role in innate immunity.It is secreted by CD4Th1 and CD8 cytotoxic T cells during antigen-specific immunity.IFN-γ or type II interferon plays an important role in innate A immunity and adaptive immunity against viral and certain bacterial and protozoan infections.IFN-γ is an important activator of macrophages and an inducer of type II major histocompatibility complex (MHCII) expression.Abnormal expression of IFN-γ is associated with many autoinflammatory and autoimmune diseases.In addition to direct inhibition of viral replication, IFN-γ is important to the immune system in terms of its immune-stimulating and immune-regulatory functions.
IFN-γ is a marker of disease in many different pathological conditions, including infections, autoimmune diseases, transplant rejection, allergic reactions, and diabetes mellitus.

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SKU: 78532156706

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Kimberly Wiley
Fort Morgan, US
★★★★★ 5
Works well, easy to use
Great little hand grinder. So much easier to use than the last one I purchased. I moved and both of my electric grinders & my hand grinder are still somewhere in storage. This easily portable gadget has been a lifesaver at a decent price. Seems sturdy enough after using it a few weeks.
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Reviewed in the United States on May 27, 2026
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Grady Peterson
Carnegie, US
★★★★★ 5
Better than expected
This thing is great. I wasn't sure what to expect for the price, but it exceeded my expectations in every way. I have no complaints, quality is fine, the grind is fine, it's easy to use. I've used mine every day since I purchased it and it works good as new. This is my first manual burr grinder, so there could very well be better ones out there, but I am very happy with this purchase.
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Reviewed in the United States on May 27, 2026
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Adam Shoulders
Houston, US
★★★★★ 4
A good little grinder for small batches.
It's a good quality grinder, I use mine for my moka pot since the grind needs to be specific. It's very easy to use and its small enough to store away. The only issue is it takes some time and effort to grind for more than one person, so keep that in mind if you're brewing anything more than a cup of espresso or a moka pot. I've dropped mine more than I can count so store on its side (although its very sturdy and was not broken) A solid product.
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Reviewed in the United States on May 11, 2026
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Jarson
Los Angeles, US
★★★★★ 5
Good quality and price
I purchased one in January and it works great, I like to have my coffee beans ground fine and It gives it fine. Just orered 2nd one for my friend.
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Reviewed in the United States on May 30, 2026
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Kayla
Fort Morgan, US
★★★★★ 5
Works very well as a pill grinder!
One of the clients I work with needs his pills crushed and dissolved. We have relied on the spoons method for years but some of his newer medications don’t crush well enough using that method. We tried the basic plastic pill crushers but they tend to get gummed up and don’t actually pulverize the pills. This fit our needs perfectly and the pills get crushed into a fine powder with ease. It is very easy to use and clean and takes very little effort to operate (at least for pills).
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Reviewed in the United States on April 17, 2026

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