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Description
Alexa Fluor® 647 Rabbit Anti-Human IFN-γ Antibody (S-212-145)Product Specification Host Rabbit Antigen IFN Synonyms Interferon gamma; IFN gamma; Immune interferon; IFNG Immunogen Recombinant Protein Location Secreted Accession P01579 Clone Number S 212 145 Antibody Type Recombinant mAb Isotype IgG Application ICFCM Reactivity Hu Positive Sample NK 92 Purification Protein A Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability &
Product Specification
| Host | Rabbit |
| Antigen | IFN-γ |
| Synonyms | Interferon gamma; IFN-gamma; Immune interferon; IFNG |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P01579 |
| Clone Number | S-212-145 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | NK-92 |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1.25μl per million cells in 100μl volume | Hu |
Background
Interferon gamma (IFN-γ) is a dimerized soluble cytokine and the only member of the type II class of interferons. It is primarily produced by CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The IFN-γ protein is encoded by the IFNG gene in humans and consists of approximately 160 amino acids, forming a noncovalently linked homodimer with a molecular weight of about 50 kDa. It plays a crucial role in regulating both innate and adaptive immunity by activating signaling pathways, such as the JAK-STAT pathway, in cells like macrophages and B cells. IFN-γ has antiviral, antibacterial, and antitumor properties and is involved in promoting inflammation and cell proliferation. However, its pleiotropic nature also means it can induce immunosuppressive effects, which limit its clinical use.
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