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Description
Rat CAPN2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a calpain 2 (CAPN2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of calpain 2 (CAPN2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Calpain 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Calpain 2 (Calpain2) is a protein encoded by the CAPN2 gene. Calpains are calcium-activated neutral proteases and non-lysosomal, intracellular cysteine proteases. This ubiquitous enzyme consists of heterogeneous forms with different large catalytic subunits associated with a common small regulatory subunit. The gene encoding the large subunit of the ubiquitous enzyme, calpain 2, has multiple heterogeneous transcription start sites in its 5' UTR. It has been shown to interact with Bcl-2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
There have to be other places to save on your camping budget
Style: 15' Rhino Sewer Hose
After 5 years of regular use with my original Camco RhinoFLEX sewer hose, I decided to proactively get another one and retire the OG to the inside of the bumper to keep as an emergency use hose.
Did I consider purchasing other brands and hoses to save a few $$$? Yes I did. Then I thought about never having to clean up a stinky black tank mess and promptly bought another one. If it ain't broke - or hasn't broke after five years of use - then don't fix it, and by that I mean don't overthink it. RhinoFLEX FTW.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
★★★★★ 5
Awesome filter! Nasty campground water no longer...
Style: Standard Filter
I have run these for years in my RV and they work killer! Here's some more in-depth thoughts:
Pros:
- These work better than I had expected. Just hook them up to the water source, hook your hose to it, run it to your trailer and viola` clean and good tasting water. Some of the places we have visited have had water that I wouldn't feed to my worst enemy. With this thing attached, it's very good tasting.
- I like the hose attachment that comes with this to aide in adding flexibility to the system and not put too much strain on the system. I have bought so many of these, I now use one attaching the filter to the nozzle, one out of the filter to my hose, and one attaching my hose to the trailer.
Notes:
- I will note that the manufacturer suggesting using these for only 1 camping season, and this makes sense. That's what I do, and it seems to work out great. It's not too much $ to replace every season.
- Another note, when first putting this filter into service (just the first time of the season, subsequent hook-ups wont do this) there will be a bunch of carbon (black in the water) that comes out of the faucet in your trailer. This is perfectly fine, safe, and of no issue. This is the filter breaking in and getting the extra carbon out of the system. There is no health concern with this happening. I just run the faucet until it clears up, and then we're good to go for the rest of the season.
- Last note, when we unhook from our camping spot, I swing the filter in my hand with my arm extended (using centrifugal force, not centrifical, look it up) to get the residual water out of the filter so it doesn't sit in there and spoil. I do this until I get most of the water drops to stop, leave it out on the counter to dry, and boom, it's perfect the next time.
Cons:
- None to report
Overall this filter does a great job taking care of some seriously nasty campground water that we've encountered all over the Southwest US. I cannot recommend it any higher.
I hope I have helped your purchasing decision of this product. I am not affiliated with the manufacturer, seller or any other product related company nor am I under any obligation to write this review. I do this for my own fun and to help others in the Amazon Community! Feel free to comment and let me know if I have helped you, and please note below if this review was able to help. If you should have any questions, please post them below in the Comments. I try to answer all inquires in a timely fashion to the best of my ability.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2017
★★★★★ 5
Clean, Great-Tasting Water Made Easy with Camco Tastepure
Style: Standard Filter
I installed the Camco Tastepure RV inline water filter on our rig, and it’s another example of why I’ve come to trust Camco products. The reinforced strain relief hose is a small but incredibly smart feature—it keeps the connection between the filter, hose, and RV kink-free and easy to manage. Setup was simple, and the whole system feels sturdy and well-designed.
A tip I’ve learned through experience: before putting a new filter into service, run water through the internal charcoal filter for 30 - 45 seconds. This flushes out any loose charcoal bits and ensures the first water you drink is crystal clean and tastes amazing. After that, connecting to the RV is straightforward, and you immediately notice how much better the water tastes.
Whether filling up a glass or cooking, it’s made a real difference in our RV experience. Reliable, easy to use, and excellent tasting water every time - I highly recommend this to any RV owner who cares about water quality on the road.
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Reviewed in the United States on January 10, 2026
★★★★★ 5
Works as designed
Style: Standard Filter
Works as designed. No issues or defects.
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Reviewed in the United States on June 2, 2026
★★★★★ 4
works well for camping
Style: Standard Filter
I’ve used this filter on my camper for years without issues, so I installed one in my outdoor kitchen at home. It was connected to a hose bib and left under constant pressure, feeding a small electric water heater for a sink.
That turned out to be a mistake. Overnight, the top of the filter housing cracked and leaked water throughout the cabinet. No idea how long it was spraying.
This filter works fine for intermittent use (like camping), but it does not appear to be designed for continuous pressure. I would not recommend installing it in a permanent, always-pressurized setup.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 14, 2026
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