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Description
Human Pancreatic Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
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| Description |
Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.8 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 5
Fantastic!!
Color: Dark Brown, Size: 10
This is the sharp, classy look you want for your child in a child’s suit! It is a nice true fit with a classy look. My son loves this and he was happy with the fit. He is a picky little guy and I was very happy with his response to the fit. The pants had a little glitch but that's ok! I’m so impressed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 1, 2025
★★★★★ 5
Perfect formal suit!
Color: Black, Size: 16
Great quality and fits perfectly!! I love the vest !!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Perfect Fit and Great Quality!
Color: Light Royal Blue, Size: 16, Color: Light Royal Blue, Size: 16
I bought this suit set for my grandson, and it exceeded all expectations. The fit was absolutely perfect—true to size and very flattering. The material feels high-quality and well-made, with attention to detail in the stitching and finish. He looked so sharp and felt comfortable wearing it, which made the occasion even more special. Highly recommend this set for any formal event!
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Reviewed in the United States on October 29, 2025
★★★★★ 5
Great value
This suit was the cutest for my son’s graduation. He is slim enough to fit a 5 but tall enough for a 6. I did get size 5, it fit good, except the vest was a bit on the smaller side. Luckily my boy is skinny. The quality is great. Definitely get your moneys worth. The only con I have is that this item was definitely worn before & likely returned to Amazon. The shirt was wrinkled and not packaged like a “new item”. It was in a regular plastic. You can see in the photo the wrinkles on the shirt sleeves.
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Reviewed in the United States on May 31, 2025
★★★★★ 4
Nice suit
This 5 piece set is really nice
Pros:
fits true to sz which allows it to sit nicely
The pants have adjustable waist bands
The color is as described
Material/fabric is not that great but will do (select a neutral color which can prevent it from looking cheap)
Perfect for a growing boy who will need another one soon
Cons:
The tie is little short and is a non adjustable elastic
the handkerchief/pocket square does not fit in the faux pocket.
If you want an elevated look - wear with a different tie, thicker fabric white shirt and nice shoes
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Reviewed in the United States on April 5, 2026
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