Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse CAMK II ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Calmodulin-dependent protein kinase II (CAMK II). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Calmodulin-dependent protein kinase II (CAMK II) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Calmodulin-dependent protein kinase II ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Calmodulin-dependent protein kinase II (CAMKII) is a serine/threonine-specific protein kinase regulated by the Ca2+/calmodulin complex. It participates in numerous signaling cascades and is considered a key mediator of learning and memory. Its dysregulation has been linked to Alzheimer's disease, Angelman syndrome, and cardiac arrhythmias. It accounts for 1-2% of all proteins in the brain and has 28 different isoforms. These isoforms are derived from alpha, beta, gamma, and delta genes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.7 ★★★★★
Based on 14 reviews
Sort
Product Reviews
★★★★★ 4
Durable But Quirky Interactive Ball
Color: Blue
I am 30 now and my tolerance for toys that break in 2 minutes is basically zero. I picked this up during a weekend DIY project when I was fixing the deck and needed something to keep the dog occupied. The E TPU material is straight up impressiveuch abuse it can take from a large breed. My dog tends to destroy generic store brand balls but this one survived being dropped down the stairs and tossed against the siding. It is comfortable enough to use daily without feeling like it is going to shatter or hurt the dog.
The internal compartment where you charge it is smart and those little pockets for the charging port are actually useful sized for my fat fingers. The smart movement is a game changer for keeping a high energy dog moving when I am busy with home improvement tasks. I was slightly worried about the 8 hour battery life but it holds a charge well enough for a few play sessions. My whole household uses this now to entertain the dog as he will not leave us alone otherwise. It works best on my kitchen tiles but it barely moves on the thick rug in the living room which is frustrating.
The vibration is loud enough to be annoying when I am trying to focus on work. It is a solid 3 star product as it does what it says but has some quirks that make it less than perfect. Spending 19.99 is fair for the durability you get compared to cheaper options. I like that it is USB rechargeable instead of eating through batteries. It is not perfect but it serves a purpose in my chaotic house.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 28, 2026
★★★★★ 5
Engaging and Durable: High-Energy Play with a Caveat for Sensitive Pups
Color: Blue, Color: Blue
Finding an automated toy that can survive the jaws of an aggressive chewer while keeping them physically engaged is a major win for busy pet owners. This smart interactive dog toy offers an excellent, high-tech way to keep high-energy dogs active, though its enthusiastic movement might be a bit intense for more timid pets.
Dynamic and Instinct-Triggering Movement: The core appeal of this toy is its smart interactive movement. This automatic bouncing ball activates immediately upon touch, rolling and bouncing in entirely unpredictable directions. This erratic motion successfully triggers a dog's natural chasing instincts, keeping them thoroughly engaged and entertained for hours, which is incredibly helpful when you are unavailable to play. However, because the movement is so sudden and dynamic, it can occasionally scare more sensitive or easily startled dogs.
Chew-Proof and Safe Construction: The durability and structural safety of the ball are highly impressive:
*Chew-Proof E-TPU: Crafted with a high-density E-TPU shell, the toy is specifically built to withstand aggressive chewing and rough play from medium to large breeds. It holds up remarkably well against scratches and deep bites for long-lasting use.
*Pet-Friendly Materials: The non-toxic, BPA-free material is completely safe and remains gentle on your dog's teeth and gums during fetch or heavy chewing sessions.
*Injure-Free Design: The toy is engineered with smooth, rounded edges to prevent any accidental cuts or injuries during high-speed play.
Supporting an Active Lifestyle: This toy serves as a fantastic tool for supporting an active lifestyle. It encourages vital daily exercise to help maintain your dog's physical health, significantly reduces boredom, and curbs destructive behaviors like furniture chewing. This makes it an absolute lifesaver for high-energy pups that need a constant physical outlet.
Convenient USB Rechargeability: Powering the toy is simple and environmentally friendly. It is equipped with a USB rechargeable battery that delivers up to 8 hours of continuous play on a single charge. It also includes the necessary USB charging cable, ensuring you can quickly plug it in and have it ready for the next play session without constantly buying disposable batteries.
Bottom Line: I highly recommend this smart interactive dog toy for owners of high-energy, medium-to-large breeds that need an indestructible, engaging outlet. While it may frighten more timid or anxious dogs due to its unpredictable bouncing, its premium E-TPU durability, great battery life, and excellent exercise value make it a fantastic investment for a healthier, happier pet.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
Ymswznwe Smart Interactive Dog Toy, Pet Automatic Moving Bouncing Ball, Chew-Proof
Color: Blue, Color: Blue
This toy has been so much fun to watch my dogs interact with. I love the randomness of the movements because it keeps them guessing and keeps the toy interesting instead of becoming predictable after five minutes.
They definitely weren’t sure what to think of it at first, but once they figured it out, it became hilarious entertainment. One of my dogs especially likes to hold it in his mouth and just let it vibrate, which cracks me up every time.
I think toys like this are great not only for physical activity, but also for mental stimulation and curiosity. It gets them engaged in a different way than a regular ball or chew toy.
While I wouldn’t consider my dogs “power chewers,” toys usually do not last long around here, so I was pleasantly surprised by how well this has held up. At the time of this review, it still looks basically brand new, so it is pretty durable.
I also really appreciate that it’s rechargeable. I’m glad I’m not constantly going through batteries with a toy like this.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 11, 2026
★★★★★ 2
Interesting Premise
Color: Blue, Color: Blue
It's an interesting premise for a toy -- it has a vibrating gyrator in the middle which turns on randomly for random durations. This then causes the ball to vibrate and roll around the floor. It really needs to be on a hardwood floor to move, though -- when I put it on their dog bed, it vibrates but then doesn't move anywhere.
I tried a few times to get my four dogs to interact this this ball toy, but they are clearly not interested.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 20, 2026
★★★★★ 5
Great toy for a dog who is not afraid of noises
Color: Crab
My puppy loves this thing! She was confused at first but now she plays with it daily. I just let it jump and sing the first time. She looked at it with the cutest head tilt. It is a bit loud so if you have a dog that's scared of noises this might be the right toy. Now when she picks it up, I turn it on and she gets so excited! The different songs make the toy less annoying for me, lol. Honestly, anything to tire out a 4 month old puppy. She is very entertaining to watch.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
recommand products
KarmaRipple's Ebony Wood Black Rutilated Quartz 925 Sterling Silver Year Of The Snake Balance Bracelet
60.00
BlueEarth L-Arginine 5000mg + L-Citrulline 2000mg Berry Blend - Nitric Oxide Booster, Heart Health & Endurance - 30 Servings
91.90
NatureGenX Just One Daily Methylated Multivitamin – 60 Caps, Iron Free, MTHFR Support
89.90
KarmaRipple's Handcrafted Eight Thread Peace Weave Peach Blossom Weave Fortune Connection Bracelet
33.00
Spartan Bee Bread Chewables – Raw Organic Bee Bread Multivitamin | Energy, Immunity & Recovery
140.00